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Fostering Food Safety Monitoring in SSA


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Problems in containing aflatoxins,Various alfatoxin quantification systems,ELISA: Ideal test for Sub-Saharan Africa,ELISA and data analysis,Quantification of aflatoxins in different commodities

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Fostering Food Safety Monitoring in SSA

  1. 1. Fostering Food Safety Monitoring in SSA P Lava Kumar and R Bandyopadhyay Reduced ability to Malnutrition cope with diseases, Quality reduction especially HIV/AIDS Aflatoxin Liver cirrhosis, immuno-suppression, contamination blocks nutrient absorption, growth Trade in food and feed abnormalities, etc. Restrictions Synergistic interaction Liver cancer with Hepatitis-B & C
  2. 2. Problems in containing aflatoxins• The most effective measures to control aflatoxins depends on a program that “monitors” their presence in food and feeds. But, • Monitoring programs in SSA relies on importation of diagnostic tools, which are expensive, some have short shelf-life and prohibitive for majority of the stakeholders.• “Sustainability” depends on the -Sustainable access to basic reagents at affordable costs. -Suitability for application in a minimally equipped labs.
  3. 3. Various alfatoxin quantification systems Analytical Methods TLC HPLC LC-MSSerological Methods Immuno-columns ELISA Lateral Flow Strips
  4. 4. ELISA: Ideal test for SSA• ELISA have been proven to be easy for adoption in developingcountries. Afla-ELISATM is a simple and low-cost test for quantitative estimation of aflatoxins developed at IITA.• IITA has developed cheap and high-through put screening systems which dramatically reduces the phenotyping cost.
  5. 5. Afla-ELISA kitComponents-AFB1-BSA standards -Coating buffer concentrate Coast:-AFB1 standards -Dilution buffer concentrate US$ 2 per sample-AFB1 polyclonal antibodies -Substrate buffer concentrate-Sample preparation & ELISA protocol -Wash buffer concentrate
  6. 6. Three simple steps1. Sample Extraction2. ELISA3. Data input into software
  7. 7. Sample extraction 3. Mix the suspension (30 min) 4. Filter the extract1. Ground 20 g sample (maize) 2. Resuspend in 100 ml of 0.5% KCL in 70% Methanol 5. Use it in ELISA
  8. 8. ELISA and data analysis Final Reaction Measure in an ELISA plate reader set with 405 nm3.000 y = -2.1638x + 2.28692.000 2 R = 0.99531.000 Input data into XL program sheet0.000 0.000 0.500 1.000 1.500 2.000 to estimate AFB1 concentration in-1.000 unknown samples-2.000
  9. 9. Requirements for aflatoxin lab Orbital Shaker ELISA Plate readerBlender Pipette pH Meter ELISA Plates General labware and a computer
  10. 10. Quantification of aflatoxins in different commodities millet 1.800 sorghum 1.600 ground nut 1.400 cassava 1.200 riceA405nm 1.000 maize 0.800 Buffer 0.600 0.400 0.200 0.000 1 2 3 4 5 6 7 8 9 Log AfB1(ng/ml)
  11. 11. Specificity of Afla-ELISA 120.0 100.0 80.0 B1% binding B2 G1 60.0 G2 FB1 40.0 OTA 20.0 0.0 1 2 3 4 5 LogAfB1(ng/ml)
  12. 12. Trade Mark Registration TM Afla-ELISA Next step: Product registration
  13. 13. Next Steps and Exit Strategy 1. Research  2. Product Development [OUTPUT]  ExitIITABy By 3rd party 3. Product Deployment [OUTPUT] Fostering utilization by NARS, researchers, monitoring agencies etc) -Product registration - Accreditation - policy -Training and facilitating infrastructure development Definite Exit 3. Utilization of Afla-ELISA for food safety monitoring [OUTCOME]
  14. 14. Thank youA&H Program R4D Week 2009