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Biotechnology and Genetic Improvement


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Presentation during IITA R4D week 2015 (23 - 27 Nov. 2015). By: Robert Asiedu.

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Biotechnology and Genetic Improvement

  1. 1. A member of CGIAR consortium www.iita.orgwww.iita.orgA member of CGIAR consortium Biotechnology and Genetic Improvement Robert Asiedu 25th November 2015 (R4D Week 2015)
  2. 2. A member of CGIAR consortium Biotechnology and Genetic Improvement R4D Week 2015
  3. 3. A member of CGIAR consortium Strategic Objectives: 1. Enhancing the management, characterization, and use of plant genetic resources 2. Enhancing the efficiency and effectiveness of plant breeding 3. Efficient diagnosis and monitoring of biological systems Biotechnology Strategy Cryo Bank
  4. 4. A member of CGIAR consortium Genetic Improvement Strategy Strategic Objectives 1.Increased yields of staple crops 2.Increased resilience of staple crops to biotic and abiotic stresses 3.Increased nutrient density and suitability of staple crops to new utilization pathways 4.Raised efficiency of breeding staple crops 5.Enhanced germplasm of staple crops and increased potential of selected underutilized crops
  5. 5. A member of CGIAR consortium • Management and utilization of genetic resources • Application of tools and methods for genotyping and phenotyping • Transgenic approaches • Data capture and management systems • Strengthening partnerships and capacity building • Deployment of knowledge and products Enabling resources and technologies
  6. 6. A member of CGIAR consortium Cassavabase: A global open access resource All data available with Open Access with agreement to observe the Toronto Protocol regarding use of unpublished data Databases
  7. 7. A member of CGIAR consortium Regional Cassava Breeders’ Meeting – West Africa and DRC
  8. 8. A member of CGIAR consortium 1. Integrated Genotyping Service and Support (IGSS) (BecA- ILRI Hub) 2. Shared Industrial-Scale High-Throughput & Low cost Genotyping Facility (ICRISAT ) 3. Integrated Breeding Platform (including the Breeding Management System) 4. Improving Breeding Program Effectiveness with the Breeding Program assessment Tool (University of Queensland ) 5. Genetic Gain Platform? Other Initiatives
  9. 9. A member of CGIAR consortium Crop Country No. of Varieties Released with Partners 2013 2014 2015 Cassava Liberia - 3 - Nigeria - 3 - DRC 6 - - Sierra Leone - 2 - Tanzania - - 4 Uganda - - 2 Cowpea Nigeria - - 2 Sierra Leone - - 2 Burkina Faso 2 - - Swaziland - - 6 Tanzania 2 - 2 Maize Benin - - 6 Ghana - - 5 Mali 2 6 - Nigeria 5 4 4 Soybean Nigeria - 2 - Sierra Leone - - 4 Zambia - - 2
  10. 10. A member of CGIAR consortium • Post-release – Seed companies [maize] – Community-based seed production [cowpea, soybean, maize] – Outgrower schemes/ development agencies – Bilateral projects [cassava (5CP, Hplus, seed syst. Nigeria), yam, cowpea, maize]
  11. 11. A member of CGIAR consortium Sensitive & Robust Virus Diagnostic Tools DevelopedType Purpose Stage of development Multiplex RT-PCR Simultaneous detection of 3 RNA viruses Completed Direct Binding PCR Episomal yam badnavirus detection Validation Antibodies for YMV, YMMV and Badnavirus ELISA, IC-PCR and LFD based diagnostics Validation RT-LAMP and RPA On-site tool kit Validation & standardization for on-site use Single tube multiplex PCR RT-LAMP
  12. 12. A member of CGIAR consortium 1. Sampling yam young vines 2. In vitro introduction Meristem excision, culture and plantlet re-growth (2 to 6 months) Bulk propagation of meristem cultures by nodal cuttings (2 - 3 months) Tuber planting in field or screenhouse. Virus indexing and selection for in vitro induction (1 to 3 months) 3. In vitro nodal cultures Virus indexing Virus indexing by multiplex RT- PCR on in vitro samples (1 week) Re-indexing for reconfirmation (2 to 6 months) 4. Acclimatization for re-indexing Bulk propagation of virus-free plants by in vitro nodal cuttings or vine propagation (2 to 4 months) 5. Selection of virus-free plants & bulking Establishing clean seed stocks
  13. 13. A member of CGIAR consortium Temporary Immersion Bioreactor Aeroponic System •Circumvents dormancy period •Links to vine propagation •Perennial growth habit •Pre-basic and basic seed production Rapid Propagation
  14. 14. A member of CGIAR consortium • Crop breeding programs • Phenotyping – Stresses; GIS; Morphology; Nutritional & end-use quality • Genotyping, Data Management & Mechanization • Cross-cutting topics – Private sector breeding; Delivery of products; Communication • Satellite Meetings – QTL, MAS, BMS – Epigenetics and Gene Expression – Breeding Values Workshop on Genetic Improvement – Sept.
  15. 15. A member of CGIAR consortium Genebank data and information management system Marimagne Tchamba Recent advances in phenotyping advanced drought tolerant and striga resistant maize genotypes for tolerance to combined drought and heat stress Silvestro Meseka Regeneration and genetic transformation of yam Rajesh Manoharan Towards molecular breeding in banana: challenges and opportunities working with perennial polyploid crops Allan Brown Enhancing proVitamin A in cassava breeding using new tools Elizabeth Parkes Presentations
  16. 16. A member of CGIAR consortium Thank you