Presentation yukiko naruoka

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  • Presentation yukiko naruoka

    1. 1. Identification and evaluation of QTL for adult plant resistance to stripe rust in the US PNW winter wheat Yukiko Naruoka Washington State University 4/29/2014
    2. 2. SWW HRW HRW HRS SRW Wheat production area and stripe rust epidemics http://www.ers.usda.gov • Pacific Northwest is a major winter wheat production area • Major stripe epidemic regions are the Pacific and Central and Southern plains
    3. 3. Top 5 PST races in WA Top 5 PSTv races in PNW Prevalence rank 2003 2004 2005 2006 2007 2008 2009 2010 2011 2012 1 100 100 115 114 114 114 139 11 11 37 2 98 102 100 100 116 116 140 41 14 48 3 80 92 102 35 115 100 114 14 4 11 4 97 101 114 117 111 127 116 37 43 53 5 25 114 116 115 98 54 127 17 17 4 # new races 6 2 5 1 6 1 1 (16) 6 2 Race frequencies and transitions in PNW http://striperust.wsu.edu/index.html • Rust virulence is changing fast and new races arise every year
    4. 4. Durable rust resistance in PNW • Incorporating only single race-specific seedling resistance is not reliable • Adult plant resistance (APR) may be inadequate under cool wet summers (effective typically >25C°) • Accumulation of effective resistance for both seedling and APR in a cultivar seems to be the best strategy for durable rust resistance in PNW
    5. 5. PNW wheat and their possible source of APR • Historically APR have been incorporated in PNW winter wheat breeding programs from Brevor and multiple other sources Chen 2013 1947 1960 1970 1980 1990 2000 Brevor Nugaines Luke 1949 1960 1965 1970 1977 Stephens Hyslop1971 1976 Raeder Daws Lewjain1982 McDermid Hill 811983 Dusty1985 Sprague1972 1984 Gaines John BatumMalcolm 1988 Madsen 1986 Oveson Eltan1990 Kmor Macvicar Bonneville1991 1992 Rod 1993 Rohde 1994 Lambert 1998 HillerCodaWeatherford 1997 Boundary 2001 Bruehl Basin FinchChukar Cappelle Desprez GaryBrundage 96 2002 Tubbs 2000 Hubbard 2004 Masami Edwin 2005 MDM Bauermeister 2007 Xerpha Nord Desprez 2006 Darwin Alpowa 1979 Walladay Wawawai Express Frontana Louise 1987 WakanzSpillman Otis Year release Wheat cultivars and their possible source of HTAP resistance BrevorBrevor NugainesNugaines Luke 1949 1960 1965 1970 1977 StephensStephens HyslopHyslop1971 1976 RaederRaeder DawsDaws LewjainLewjain1982 McDermidMcDermid Hill 81Hill 811983 DustyDusty1985 SpragueSprague1972 1984 GainesGaines JohnJohn BatumBatumMalcolmMalcolm 1988 MadsenMadsen 1986 OvesonOveson EltanEltan1990 KmorKmor MacvicarMacvicar BonnevilleBonneville1991 1992 RodRod 1993 RohdeRohde 1994 LambertLambert 1998 HillerHillerCodaCodaWeatherfordWeatherford 1997 Boundary 2001 BruehlBruehl BasinBasin FinchFinchChukarChukar Cappelle DesprezCappelle Desprez GaryGaryBrundage 96Brundage 96 2002 TubbsTubbs 2000 HubbardHubbard 2004 MasamiMasami EdwinEdwin 2005 MDMMDM BauermeisterBauermeister 2007 XerphaXerpha Nord Desprez Nord Desprez 2006 DarwinDarwin AlpowaAlpowa 1979 WalladayWalladay WawawaiWawawai ExpressExpress FrontanaFrontana LouiseLouise 1987 WakanzWakanzSpillmanSpillman OtisOtis Year release Wheat cultivars and their possible source of HTAP resistance Dr. Orville Vogel Photo courtesy: WSU CAHNRS
    6. 6. Objective • Identifying QTL and molecular markers linked to those stripe rust resistances by genome wide association mapping (GWAS) • Evaluate the effect of APR pyramiding in PNW winter wheat germplasm
    7. 7. QTL identification • Germplasm: – 402 accessions consisted of club and common winter wheat adapted to PNW selected from 17 breeding programs • Phenotyping: – Field evaluation: 3 sites for two years in WA – Greenhouse evaluation: Pstv-11 and Pstv-37 (predominant), and Pstv-51 (new) • Genotyping: – 9K Illumina SNP array (5,777SNP used) and 12 SSRs and STS linked to known genes and QTL • Association analysis: – MLM with Q (PC) and K (kinship) matrices performed by TASSEL v.3.0 – Qvalue (FDR=0.1) was used for significant test – QTL were determined based on cM and LD between significant markers and other information from marker-trait association
    8. 8. Significant QTL for strip rust reactions through GWAS 1B 10.7 22.6 cM 26.3 11.6 18.1 1D 15.5 cM 2D 0 cM 154.8 IT DS 2B 15.1 37.9 cM IT DS 4A cM 65.7 IT DS 4B cM IT DS 68.3 105.6 110.6 6BcM IT DS 112.3 150.0 :Pul1-2012 :Pul2-2012 :CF-2012 :Pul1-2013 :CF-2013 :MV-2013 :Pstv-37 :Pstv-51 :Pstv-11 QYrst.orr-2B.2 QYrco.wpg- 1B.1 Case et al. 2014 Vazquez et al. 2012 2A 9.6 11.1 IT DS gwm359 29.2 243.8 cM Yr17Ventriup/LN2 QYrst.orr-2AS
    9. 9. Selection of genotypes carrying only APR • 366 Genotypes carrying at least one seedling gene or QTL; Yr17, the 1BS and 1DS QTL • The rest of the 36 genotypes were evaluated for the effect of APR accumulation by linear regression analysis • IT and DS averaged over all environment were used for the analysis
    10. 10. The effect of APR pyramiding 0 2 4 6 8 10 0 1 2 3 4 5 6 7 8 9 10 11 12 13 y= -0.40 X + 6.94 R2 = 0.37 0 20 40 60 80 100 0 1 2 3 4 5 6 7 8 9 10 11 12 13 y= -6.05 X + 67.63 R2 = 0.49 # of Resistant allele # of Resistant allele IT DS • Significant linear regression (p≤0.0001) was found for both IT and DS
    11. 11. 0 2 4 6 8 10 0 1 2 3 4 5 6 7 8 9 10 11 12 13 IT # of Resistant allele AP700CL Stephens ORCF-103 The effect of APR pyramiding to yield • Dr. Xianming Chen (USDA ARS) has conducted an experiment to estimate yield loss due to stripe rust by spraying fungicide since 2002 • Yield loss (%) =(yield w/ fungicide - yield w/o fungicide)/ yield w/ fungicide x 100
    12. 12. The effect of APR pyramiding to yield Resistant combination Cultivar # of APR % of yield loss by stripe rust 2011 2012 2013 APRonly AP700CL 12 9.0* 3.3 -4.7 APRonly Stephens 6 12.6 14.0* 5.3 APRonly ORCF-103 5 32.5* 21.3* 24.0* - APR+Seedling PS279 (0) 89.8* 57.5* 34.2* Madsen 7+1 -1.2 2.2 3.2 Days > 25C° May-July 21 32 45 http://striperust.wsu.edu/index.html • Increased number of APR correlated with decreased yield loss by stripe rust. • Under the high inoculum and cool, wet summer (2011), APR alone did not seem to give enough resistance (9% yield loss = $85million) *: p<0.05 for yield difference between control (w/o fungicide) and treatment (w/ fungicide)
    13. 13. Summary • Multiple QTL associated with stripe rust response were identified through GWAS • Pyramiding APR alleles showed significant effect on stripe rust response and decrease of yield loss by stripe rust • Under cool, wet environments, a cultivar carrying both seedling resistance and APR showed no yield loss • Pyramiding effective seedling and APR using molecular markers would facilitate durable rust- resistant winter wheat cultivar development
    14. 14. Acknowledgement Arron Carter Kim Campbell Mike Pumphrey Xianming Chen Deven See All staff in winter wheat and spring wheat genetics lab and USDA wheat genetics, quality physiology and disease research group Funding: Washington Grain Commission Project #5238 National Research Initiative Competitive Grants CAP project 2011-68002-30029 from the USDA National Institute of Food and Agriculture
    15. 15. Thank you for your attention!

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