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1. Place the Paraffin infiltrated tissue cassettes   in Tissue Embedding Station (TES) ’s Paraffin   Bath at 65° C for 15 ...
4. Place the empty mold on top of hot plate of TES5. Pour a little Hot Paraffin from the reservoir of TES   into the mold ...
8. Shift the mold from the hot plate to the cold  plate (-15°C) of Tissue Cooling Station9. When the wax is hard, take out...
Tissue Embedding Procedure
Tissue Embedding Procedure
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Routine histological techniques
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Tissue Embedding Procedure

List of steps to be followed for Paraffin Embedding of Tissues in Histology Labs

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Tissue Embedding Procedure

  1. 1. 1. Place the Paraffin infiltrated tissue cassettes in Tissue Embedding Station (TES) ’s Paraffin Bath at 65° C for 15 minutes.2. Warm the metal block molds in the warm storage area of TES3. Remove the paraffin infiltrated tissue from the tissue cassette and using a blade dissect out the infiltrated tissue.
  2. 2. 4. Place the empty mold on top of hot plate of TES5. Pour a little Hot Paraffin from the reservoir of TES into the mold and place the tissue specimen on top of it6. Using heated forceps orientate the tissue specimen as desired and pour hot paraffin7. Place the labeled empty tissue cassette on top of mold and pour some more hot paraffin so as to cover plastic cassette
  3. 3. 8. Shift the mold from the hot plate to the cold plate (-15°C) of Tissue Cooling Station9. When the wax is hard, take out the paraffin block from the mold10. The paraffin block is now ready for sectioning
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List of steps to be followed for Paraffin Embedding of Tissues in Histology Labs

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