Components Of Blood (For Transfusion) • Each unit of blood is tested for evidence of hepatitis-b, hepatitis-c, Human Immune deficiency Virus I & II. • The blood is then processed into sub-components. • Whole blood • Packed cell volume • Fresh frozen plasma • Platelets • Cryoprecipitate

1. Components Of
Blood (For Transfusion)
Mr. Harshad Khade
MSc. Medical Technology (OTA)
Symbiosis International university, Pune.

3. Components Of Blood (For Transfusion)
• Each unit of blood is tested for evidence of hepatitis-b,
hepatitis-c, Human Immune deficiency Virus I & II.
• The blood is then processed into sub-components.
• Whole blood
• Packed cell volume
• Fresh frozen plasma
• Platelets
• Cryoprecipitate

5. Whole Blood
• Whole blood is unseparated blood containing an
anticoagulant – preservative solution.
• One unit of whole blood contains-
• 450 ml of donor blood.
• 50 ml of anticoagulant-preservative solution.
• Haemoglobin approx.12g/ml & haematocrit 35%- 45%.
• No functional platelets.

6. • Stored between +2 and +6 degrees centigrade in a blood
bank refrigerator.
• Transfusion should be started within 30 minutes of removal
from the refrigerator and completed within 4 hours of
commencement because changes in the composition may
occur due to red cell metabolism.

8. Packed Red Cells
• Packed red cells are cells that are spun down and
concentrated.
• One unit of packed red cells is approx. 330 ml and has a
haematocrit of 50-70%.
• They are stored in a SAG-M (saline-adenine- glucose-
mannitol) solution to increase their shelf life to 5weeks at +2
to +6degrees centigrade.
• 1 unit of PRBCs = raises haematocrit by 2-3%

10. Fresh Frozen Plasma
• Plasma is the liquid component of blood; it has proteins called clotting
factors
• Fresh frozen plasma is rich in coagulation factors.
• Expands blood volume and provides clotting factors.
• It is separated from whole blood and stored at-40 to -50 degrees
centigrade with a 2year shelf-life.
• Contains no RBCs .
• 1 unit of FFP = increases level of any clotting factor by 2-3%.
• It is the first line therapy in the treatment of coagulopathic
haemorrhage.

12. Platelets
• Also known as thrombocytes
• Tiny cell structures necessary in blood clotting process
• Replaces platelets in clients with bleeding disorders, or
platelet deficiency
• 1 unit = increases the average adult client’s platelet count by
about 5,000 platelets/microliter

13. • Autologous Red Blood Cells
• Used for blood replacement following planned elective
surgery
• Must be donated 4-5 weeks prior to surgery.
• Albumin and Plasma Protein Fraction
• Blood volume expander
• Provides plasma protein

15. Cryoprecipitate
• Cryoprecipitate is a supernatant precipitate of fresh frozen
plasma and is rich in factor VIII and fibrinogen.
• It is stored at -30 degrees centigrade with a 2 years shelf life.
• Indicated in low fibrinogen states (<1g/l) or in cases of factor VIII
deficiency (haemophilia-a), von will brand's disease and as a
source of fibrinogen in disseminated intravascular coagulation.
• Pooled units containing 3-6 gm fibrinogen in 200-500 ml raises
the fibrinogen level by approx. 1g/L.
• Must be infused within 6 hours.

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Thank You