Sana presentation11

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Sana presentation11

  1. 1. ANTISPERM ANTIBODYASSOCIATED WITHINFERTILITY IN MEN
  2. 2.  Infertility is defined as inability of couples to achievepregnancy following one year of unprotected intercourse. Primary infertility Secondary infertility
  3. 3.  Male infertility may be due to:1. Decrease in the number of sperms2. Sperm being blocked from being released3. Sperm that do not work properly Other causes of male infertility…..?
  4. 4.  Semen analysis…………………….male pathology. Techniques for andrology for monitoring semenparameters, like sperm concentration, motility andmorphology. Computer assisted semen analysis (CASA)
  5. 5.  CASA has categorized motility(micrometer/sec), of sperm into three classesas:1. average path velocity (VAP)2. straight line velocity (VSL)3. curvilinear velocity (VCL)
  6. 6.  Testicular pathology is divided in to subgroups as:1. Teratozoospermia2. Oligozoospermia3. Asthenozoospermia4. Azoospermia5. Polyzoospermia
  7. 7.  Antisperm antibodies are antibodies directed against thesperm. Research began in 1899 Found systematically and in local secretion. Impair the fertilizing ability of spermatozoa.
  8. 8.  To compare the data of semen parameters from fertileand infertile subject categories. To see the role of antisperm antibodies on spermmotility analyzed through CASA in fertile and fertilemale subjects. To determine the impact of ASA in our population andhow it varies in different categories of infertile malesubjects. To determine whether seminal plasma ASA can be usedas diagnostic tool in evaluating male fertility potential.
  9. 9. (n=10) fertile and (n=72) infertile malesubjects.The infertile patients were subdivided into different categories :(ATZS), (AZS), (OZS), (OAZS), (SA).Semen volume, sperm count, CASA,motility parameters were measuredaccording to WHO criteria.Antisperm antibody enzyme linkedimmunosorbent assay (ELISA) kit.
  10. 10.  There was non-significant (P>0.05) difference in mean ± SEMsemen volume among fertile and different categories ofinfertile male subjects (ATZS, OZS, OAZS, SA). However azoospermics (AZS) patients showed significant(P<0.01) decreased in semen volume as compared to fertilemen.
  11. 11. a**0123456fertile ATZS AZS OAZS OZS SASemenvolume(ml)Subjects categoriesfertileATZSAZSOAZSOZSSAa**Figure 1: Mean ± SEM semen volume (ml) of fertile (n=10) andinfertile (n=72) astheno-teratozoospermics (ATZS),azoospermics (AZS), oligoasthenozoospermics (OAZS)oligozoospermics (OZS) and sperm agglutination (SA) malesubjects.
  12. 12.  There was highly significant (P<0.001) decrease in spermcount OZS, OAZS as compared to fertile male subjects. While patients with sperm agglutination showed highlysignificant (P<0.001) increase in sperm count whencompared with fertile. There were non significant difference (P>0.05) in mean ±SEM sperm count (mill/ml) among fertile and ATZS infertilemale subjects.
  13. 13. Figure :Mean ± SEM sperm count (mill/ml) of fertile (n=10) andinfertile (n=72) astheno-teratozoospermics (ATZS),azoospermics (AZS), oligoasthenozoospermics (OAZS),oligozoospermics (OZS) and sperm agglutination (SA) malesubjects.050100150200250Fertile ATZS AZS OAZS OZS S.ASpermcount(mill/ml)Subjects categoriesFertileATZSAZSOAZSOZSS.A
  14. 14.  There was significant (P<0.05) difference in mean ± SEMsperm motility (%) among fertile and different categories ofinfertile male subjects.
  15. 15. Mean ± SEM sperm motility (%) in different classes of sperm motility (rapidprogressive motile (%), slow progressive motile (%), and non motile (%) of fertile(n=10) and infertile (n=72) astheno-teratozoospermics (ATZS), azoospermics (AZS),oligoasthenozoospermics (OAZS), oligozoospermics (OZS) and spermagglutination (SA) male subjects.0102030405060Fertile ATZS OAZS OZS AZS SASpermmotility(%)Subjects categories
  16. 16.  Curvilinear velocity (VSL µm/sec)There was non-significant (P>0.05) difference in mean ± SEMcurvilinear velocity (VSL µm/sec among fertile and differentcategories of infertile male subjects. Straight line velocity (VSL µm/sec)There was significant (P<0.05) difference in mean ± SEMstraight line velocity (VSL µm/sec) among fertile anddifferent categories of infertile male subjects.
  17. 17.  Average path velocity (VAP µm/sec)There was non significant (P>0.05) difference in mean ± SEMAverage path velocity µm/sec (VAP µm/sec) among fertileand different categories of infertile male subjects. Wobble (WOB µm/sec)There was significant (P<0.05) difference in mean ± SEMaverage path WOB among fertile and different categories ofinfertile male subjects.
  18. 18.  Amplitude of lateral head (ALH µm/sec)       There was significant (P<0.05) difference in mean ± SEM amplitude of lateral head µm/sec (ALH) among fertile and different categories of infertile male subjects.
  19. 19.  ASAs had showed highly significant (P<0.001) increased in concentration in  sperm agglutination (SA) infertile male subjects and asthenoteratozoospermics (ATZS) patients showed significant (P<0.05) increased in ASAs as compared to oligozoospermics (OZS), oligoasthenozoospermics (OAZS) and azoospermics (AZS) infertile male subjects.
  20. 20. Mean ± SEM antisperm antibodies (U/ml) of fertile (n=10) and infertile(n=72) astheno-teratozoospermics (ATZS), azoospermics (AZS),oligoasthenozoospermics (OAZS), oligozoospermics (OZS) and spermagglutination (SA) male subjects.020406080100120Fertile ATZS OAZS OZS AZS SAASA(U/ml)Subjects categories
  21. 21.  Standard semen parameters have prognostic ability to evaluate the male fertility. Seminal plasma antisperm antibodies (ASAs) showed significant increased in concentration in different categories of infertile male’s subjects. Seminal plasma antisperm antibodies (ASAs) can be used as diagnostic tool for evaluation of male infertility especially in those infertile patients having sperm agglutination (SA) and asthenoteratozoospermics infertile patients (ATZS). 

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