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Noreen moin


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Noreen moin

  1. 1. Evaluation of resistance in wheat againstRhopalosiphum padi (L.) (Homoptera: Aphididae)Noreen Moin05-arid-345PMAS-Arid AgricultureUniversity Rawalpindi
  2. 2. CONTENTSIntroductionMaterials and methodsSeedling bulk testAntibiosis testTolerance testResultsSeedling bulk testAntibiosis testTolerance testConclusionRecommendation
  3. 3. INTRODUCTIONAphids are consider as serious pestsof crops (Wheat, Barely, Maize etc)Transmit virus (MMSV, BYDV)Persist through out the wheat seasonReproduceSexuallyAsexually
  4. 4. INTRODUCTION continue…Some important species of aphids inPakistanBird cherry oat aphid (Rhopalosiphum padi (L))Wheat grain aphid (Sitobion avenae)Grain aphid (Sitobion miscanthi)Corn leaf aphid (Rhopalosiphum maidis)Control strategiesInsect pest managementBenefits of IPMEnvironmentally sound methodEconomic
  5. 5. OBJECTIVESEvaluation of resistant varieties ofNational Uniform Wheat Yeild Trials(NUWYT) Rain Fed (RF) against Birdcherry oat aphid by applying followingtestsSeedling bulk testAntibiosis testTolerance test
  6. 6. MATERIALS AND METHODSHost was wheat11 varieties of (NUWYT) Rain Fed (R.F) of year 2004-05.Experiments were conducted under controlledenvironment28+2 oC, 40-70% RH and photoperiod of 16:8hMass rearing was done in NARC by establishing theculture of Rhopalosiphum padi (L)Resistance was evaluated bySeedling bulk testAntibiosis testTolerance test
  7. 7. SEEDLING BULK TESTMetal traysmeasuring(51x35x9 cm)Eight rows of one cmdepth25 seeds per genotypewas sownTen aphids werereleased at seedlingheight of 5-6 cmDamage rating wasrecorded after 10-15days
  8. 8. ANTIBIOSIS TESTTwo seeds were sown in 7 cmdiameter potAt height of 5-6 cm, eachvariety was thinned to oneOne adult aphid was releasedCovered with plastic cagehaving two side ventilationholes, 6 cm diameter and 30cm heightOne nymph was allowed togrow until it becomes sexuallymatureNymphs reproduced daily wereremoved and counted for eachseedling
  9. 9. TOELRANCE TESTSeeds weresown asdescribed inantibiosis testSeedlings wereinfested byaphids at theheight of 5-6cmTen adultsweremaintained
  10. 10. RESULTSSeedling bulk testVarieties NUWYT (RF) No. of seedlings Damagerating (DR)Nature ofresistancePR-87 21 4 MRNR-241 17 4 MRSN-128 20 5 MRV-00BT004 16 5 MRV-00055 18 6 MRV-5 20 3 RNRL-2017 22 7 SNR-234 15 5 MR
  11. 11. RESULTSSeedling bulk test CONTI….Varieties NUWYT(RF)No. of seedlings Damage rating (DR) Nature of resistanceV-002467 15 4 MRDN-44 18 4 MRPR-83 25 5 MRChakwal-97 14 7 SR = Resistant varieties with damage rating = 3MR = Moderately Resistant varieties with damage rating = 4-6S = Susceptible varieties with damage rating =7-9
  12. 12. RESULTSANTIBIOSIS TESTVarieties NUWYT (RF) Average no. of nymphs RemarksPR-87 1.3 MFNR-241 1.3 MFSN-128 1.5 HFV-00BT004 1.3 MFV-00055 1.2 MFV-5 1.0 MFNRL-2017 0.9 MFNR-234 1.1 MF
  13. 13. Varieties NUWYT (RF) Average no. of nymphs RemarksV-002467 0.5 LFDN-44 1.2 MFPR-83 0.7 LFChakwal-97 1.6 HFWafaq-2001 1.3 MFRESULTSANTIBIOSIS TEST CONTI..LF = Least FecundMF = Moderately FecundHF = Highly Fecund
  14. 14. RESULTSTOLERANCE TESTVarieties NUWYT (RF) Average DR RemarksPR-87 3.8 MTNR-241 3.6 HTSN-128 3.6 HTV-00BT004 6.2 LTV-00055 4.4 MTV-5 3.6 HTNRL-2017 4.4 MTNR-234 5.4 MT
  15. 15. RESULTSTOLERANCE TEST CONTI..Varieties NUWYT (RF) Average DR RemarksV-002467 5.2 MTDN-44 4.6 MTPR-83 3.6 HTChakwal-97 4.2 MTWafaq-2001 4.6 MTHT = Highly TolerantMT = Moderately TolerantLT = Least Tolerant
  16. 16. 3.83.6 varieties NUWYT (RF) 2004-05 alongwith Wafaq 2001AvgDamageRatingPR-87NR-241SN-128V-00BT004V-00055V-5NRL-2017NR-234V-002467DN-44PR-83Chakwal-97Wafaq-2001
  17. 17. RECOMANDATIONSThe use of resistant varieties will remainthe most logical and economical way ofreducing damage of pests in wheatgermplasmCONCLUSIONSIn seedling bulk test V-5 was highly resistantIn antibiosis test least fecund line was PR-83In tolerance test four lines NR-241, SN-128, V-5and PR-83 was found to be tolerant to aphid R.padi