Dilnaz presentation

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Dilnaz presentation

  1. 1. Effects of Kisspeptin on plasmaEstradiol concentrations in UromastyxhardwickiiHasan Shafique and DilnazM. Phil. A/H Physiology Laboratory,Quaid-i-Azam University, Islamabad.
  2. 2. Introduction• Kisspeptins (Metastin)– KiSS-1 mRNA expression in the basal ganglia,hypothalamus, amygdala, subtantia nigra, hippocampus.– Expression in the dorsomedial hypothalamic nucleus,ventromedial nucleus and arcuate within thehypothalamus, nucleus of the solitary tract and thecaudal ventrolateral medulla.– Also in telencephalon, diencephalon, mesencephalon,metencephalon and myelencephalon.
  3. 3. Introduction• Kisspeptins (Metastin)– Product of KiSS-1 gene.– Kisspeptin-54, Kisspeptin-14, Kisspeptin-13, Kisspeptin-10– Level of activity– Binds with AXOR12/ hOT7T175/ GPR54
  4. 4. IntroductionStructure of human prepro-kisspeptin
  5. 5. IntroductionKisspeptin-induced intracellular pathway
  6. 6. Introduction• Kisspeptins (Metastin)– Effects on breast cancer, papillary thyroid carcinoma,pancreatic cancer cells, and ovarian carcinoma cells– Regulate the levels of GnRH from the hypothalamicGnRH neurons– Key regulator of hypothalamic-pituitary-gonadal axis– As a master switch for puberty
  7. 7. Introduction• Kisspeptins (Metastin)– Glucose-induced insulin secretion– Expression in coronary artery and umbilical vein– Vasoconstrictor– As an anticoagulant of blood
  8. 8. Introduction• Similar role in fish and amphibians has also been indicated• The neuroendocrine and neuromodulatory roles ofkisspeptin are presently unknown in reptiles.
  9. 9. Aims and Objectives• Acute intraperitoneal administration in investigating therole of kisspeptin-10 in modulating serum estradiol levels innon-breeding season in male Uromastyx harwickii.
  10. 10. Study Design• Group-1 Normal Control• Group-2 1 g kisspeptin• Group-3 10 g kisspeptin• Group-4 50 g kisspeptin• Group-5 100 g kisspeptin• Group-6 70 IU hCG• Group-7 100 IU hCG• Group-8 50 g kp Antagonist + 50 g kp• Group-9 50 g kp + Saline
  11. 11. Materials and Methods
  12. 12. Analysis of Results• Serum estradiol concentrations were estimated throughEnzyme Immunoassay (EIA/ELISA).• Paired ANOVA and single factor one way ANOVA
  13. 13. Results• Significant increase in serum estradiol levels uponadministration of all doses of kisspeptin (p<0.05).• Increase in serum estradiol levels upon administration ofhCG treatment groups (p<0.05).• Kisspeptin antagonist along with kisspeptin treatmentgroup.• Kisspeptin antagonist treatment group.
  14. 14. Fig 3.1 Effect of kisspeptin-10 administration at the dose of 1 μg on serumestradiol concentration in male Uromastyx hardwickii. Note significantincrease at 1 hr and 2 hrs post injection. Mean ±SEM, P< 0.001
  15. 15. Fig 3.2 Effect of kisspeptin-10 administration at the dose of 10 μg on serumestradiol concentration in male Uromastyx hardwickii. Note significantincrease at 1 hr and 4 hrs post injection. Mean ±SEM, P< 0.001
  16. 16. Fig 3.3 Effect of kisspeptin-10 administration at the dose of 50 μg on serumestradiol concentration in male Uromastyx hardwickii. Note significantincrease at 1 hr post injection. Mean ±SEM, P< 0.001.
  17. 17. Fig 3.4 Effect of hCG administration at the dose of 100 IU on serumestradiol concentration in male Uromastyx hardwickii. Note significantincrease at 30 min, 1 hr, 2 hrs and 4 hrs post injection. Mean ±SEM, P<0.001.
  18. 18. Fig 3.5 Effect of kisspeptin antagonist administration at dose of 50 μg onserum estradiol concentration in male Uromastyx hardwickii. No significantdifference was observed from control.
  19. 19. Discussion• Adult mice, sheep, cows, primates• 85% homology in rat receptor80% homolgy in mice, 40 % innon-mammalian vertebrates, such as bullfrogs.• Kisspeptin-10 profoundly affect serum concentration ofestradiol in male Uromastyx hardwickii, which clearly provesthat kisspeptin is fully active in reptiles and has roles inreptilian reproduction similar to those described inmammals, but still has to be confirmed by further scientificresearch.
  20. 20. Thank You

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