Effortless Emulsion Evaluation for Everyone

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Dr. Jeff Bodycomb from HORIBA Scientific presents information about measuring the size and zeta potential of emulsions.

Emulsion behavior is often determined by droplet size. Evaluation of droplet size by laser scattering is a time-tested method for checking product quality or performance. In addition, surface charge is often a factor in predicting emulsion stability. And that can be measured by scattering as well. Whether you are trying to create emulsions for food, pharmaceutical, or other purposes or trying to break emulsions for water treatment, this webinar is for you.

Jeff will cover the following topics in this webinar:

* Track particle size of emulsions
* Choose which measurement technology is best for your emulsion system
* Use zeta potential to more effectively break emulsions

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Effortless Emulsion Evaluation for Everyone

  1. 1. © 2013HORIBA, Ltd. All rights reserved.Effortless Emulsion Evaluation forEveryoneJeffrey Bodycomb, Ph.D.HORIBA Scientificwww.horiba.com/us/particle
  2. 2. © 2013 HORIBA, Ltd. All rights reserved.OutlineWhat is an emulsion?Measuring size->laser diffractionMeasuring size->dynamic light scatteringMeasuring charge->zeta potential
  3. 3. © 2013 HORIBA, Ltd. All rights reserved.What is an emulsion“Mixture” of two immiscible liquids suchas oil in water.Also need an emulsifier!
  4. 4. © 2013 HORIBA, Ltd. All rights reserved.Droplets are not stable due to largesurface area.Use an emulsifier to reduce surfaceenergy (surface tension)Emulsifier?H+H+H+H+----H+H+H+H+
  5. 5. © 2013 HORIBA, Ltd. All rights reserved.Examples of Emulsions Food Salad dressings Mayonnaise (oil in water,high particle conc.) Butter (water in oil) Flavoring Pharma/Personal Care Lotions/Creams Drug delivery
  6. 6. © 2013 HORIBA, Ltd. All rights reserved.Industrial Emulsions Drilling Fluid MI SWACO Schlumberger has a reversibleoil-water emulsion! Cutting Fluid Water for heat removal/environmentalfriendliness Oil for lubrication Waste Waste that includes oil droplets that areemulsified cannot be treated by simpleflotation. The emulsion should be broken.https://www.slb.com/~/media/Files/resources/oilfield_review/ors04/aut04/06_reversible_drilling.pdf
  7. 7. © 2013 HORIBA, Ltd. All rights reserved.Emulsion polymersYou often hear of emulsion polymers.When prepared, they are suspensionsof polymer particles in a liquid.One step in their preparation is thepreparation of an oil (monomer) in wateremulsion.
  8. 8. © 2013 HORIBA, Ltd. All rights reserved.What do we care about?Droplet SizeAffects mouth feel and flavor for foodAffects kinetics of chemistry (e.g., flavor ordrug release)Affects suspension viscosityAffects suspension appearanceAffects suspension stabilitySurface ChargeAffects suspension stability
  9. 9. © 2013 HORIBA, Ltd. All rights reserved.OutlineWhat is an emulsion?Measuring size->laser diffractionMeasuring size->dynamic light scatteringMeasuring charge->zeta potential
  10. 10. © 2013 HORIBA, Ltd. All rights reserved.The Measurement: LA-950 Optics
  11. 11. © 2013 HORIBA, Ltd. All rights reserved.What is Measured?Laser diffraction gives the diameter of asphere that scatters the same way asyour sample.D DD
  12. 12. © 2013 HORIBA, Ltd. All rights reserved.Mie: Effect of SizeAs diameter increases, intensity (per particle) increasesand location of first peak shifts to smaller angle.
  13. 13. © 2013 HORIBA, Ltd. All rights reserved.Water in oil emulsionOil industry
  14. 14. © 2013 HORIBA, Ltd. All rights reserved.Pharma EmulsionBimodal distribution, another water in oilemulsion.Used fraction cell for small samplevolume.
  15. 15. © 2013 HORIBA, Ltd. All rights reserved.Lotion (oil in water)
  16. 16. © 2013 HORIBA, Ltd. All rights reserved.Lotion (oil in water)-overlay
  17. 17. © 2013 HORIBA, Ltd. All rights reserved.Lotion (oil in water)-3rd repeat
  18. 18. © 2013 HORIBA, Ltd. All rights reserved.A variety of formulas
  19. 19. © 2013 HORIBA, Ltd. All rights reserved.Pop QuizLaser Diffraction can be used to measure:
  20. 20. © 2013 HORIBA, Ltd. All rights reserved.Pop QuizLaser Diffraction can be used to measure:Any emulsion, but check chemical compatibility.Instruments with chemically resistant parts areeasy, but somewhat more expensive to build.Check this point when using (or purchasing) aninstrument.
  21. 21. © 2013 HORIBA, Ltd. All rights reserved.OutlineWhat is an emulsion?Measuring size->laser diffractionMeasuring size->dynamic light scatteringMeasuring charge->zeta potential
  22. 22. © 2013 HORIBA, Ltd. All rights reserved.ParticlesZeta potentialBackscatter (173°)(High conc.)90° for size and MW, A2LaserModulatorPDFor T%532nm, 10mWAttenuatorAttenuatorParticles movingdue to BrownianmotionDLS Optics
  23. 23. © 2013 HORIBA, Ltd. All rights reserved.DLS signal Random motion of particles leads to randomfluctuations in signal (due to changingconstructive/destructive interference of scatteredlight.
  24. 24. © 2013 HORIBA, Ltd. All rights reserved.Correlation Function Random fluctuations are interpreted in termsof the autocorrelation function (ACF).)()()()()( 0tItIdttItICT )2exp(1)(  C
  25. 25. © 2013 HORIBA, Ltd. All rights reserved.What is Measured?DLS gives the diameter of a sphere thatmoves (diffuses) the same way as yoursample.Dh DhDh
  26. 26. © 2013 HORIBA, Ltd. All rights reserved.Making the Measurement1. Fill sample cell.2. Insert cell into instrument.3. Click start.4. Wait ~2 minutes.5. Review results.
  27. 27. © 2013 HORIBA, Ltd. All rights reserved.Repeats of flavor emulsion
  28. 28. © 2013 HORIBA, Ltd. All rights reserved.Compare 3 flavor emulsionsby DLS
  29. 29. © 2013 HORIBA, Ltd. All rights reserved.DLS vs Diffraction Flavor Emulsions, comparing two techniques Measuring slightly different things so results differ. Which to use? What other sizes do you want to measure? Stick with Z-average for DLS results Choose one technique and stick with it.
  30. 30. © 2013 HORIBA, Ltd. All rights reserved.OutlineWhat is an emulsion?Measuring size->laser diffractionMeasuring size->dynamic light scatteringMeasuring charge->zeta potential
  31. 31. © 2013 HORIBA, Ltd. All rights reserved.Why Zeta Potential? Good way of evaluating electrostatic stabilizationof suspensions Can use to predict interactions
  32. 32. © 2013 HORIBA, Ltd. All rights reserved.How to determine zetapotential Apply an electric field and probe response ofparticles to applied field. You need to see Doppler shift in scattered lightdue to particle motion with respect to fixedelectrodes.- +00+dV
  33. 33. © 2013 HORIBA, Ltd. All rights reserved.ParticlesFor “Zeta“LaserModulatorPMTPDFor T%532nm, 10mWAttenuatorAttenuatorUse optical mixing to extract motion ofparticles relative to electrodes.Optical System
  34. 34. © 2013 HORIBA, Ltd. All rights reserved.Zeta Potential of FlavorGood repeatability!
  35. 35. © 2013 HORIBA, Ltd. All rights reserved.ResultsIso-electric point pH 4.0●Iso Electric Point of Coffee MateEffect of Liquid-60-50-40-30-20-1001020304050602.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0pHZetapotential/mV
  36. 36. © 2013 HORIBA, Ltd. All rights reserved.Use polyions to reduce effect ofemulsifier and break emulsion oraccelerate flocculation.CoagulentH+H+H+H+----H+H+H+ H+Al+++Al+++Al+++Al+++Al+++SO4--SO4--SO4--
  37. 37. © 2013 HORIBA, Ltd. All rights reserved.PreTestWhen adding coagulant to flocculatewaste: …
  38. 38. © 2013 HORIBA, Ltd. All rights reserved.Wastewater Add proprietary coagulent to break emulsion
  39. 39. © 2013 HORIBA, Ltd. All rights reserved.DilutionSometimes samples need to be diluted.To check if the concentration is correct,do a concentration study.
  40. 40. © 2013 HORIBA, Ltd. All rights reserved.Q&AAsk a question at labinfo@horiba.comKeep reading the monthly HORIBA Particlee-mail newsletter!Visit the Download Center to find the video and slidesfrom this webinar.Jeff Bodycomb, Ph.D.P: 866-562-4698E: jeff.bodycomb@horiba.com
  41. 41. © 2013 HORIBA, Ltd. All rights reserved.Thank you
  42. 42. © 2013 HORIBA, Ltd. All rights reserved.DankeGraciasБольшое спасибоGrazie‫ر‬ْ‫ك‬ُ‫ش‬‫ا‬ Σας ευχαριστούμε감사합니다ObrigadoTacka谢谢ขอบคุณครับありがとうございましたधन्यवादநன்றCảm ơnDziękuję

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