Expression of biodegradable
plastics in plants
J.GODWIN
BTB-11-010
Introduction
Plants can be used as living factories to produce
biodegradable plastics from renewable resources.
Poly(3-hyd...
Biosynthesis of PHB
Generating transgenic plants for PHB
synthesis
Transferring the complete phb operon, consisting of the
phbC,phbA and phbB ...
Problems
There is an obvious need to improve transgenic
PHB production by producing higher
accumulation without side-effec...
Inducible trans-activation in plastids
chloroplast transit peptide sequence + ethanol
inducible promoter
Expression cassette for nuclear
genome and plastid transformation
Achievements
Targeting of the polyhydroxybutyrate biosynthetic pathway to the
plastids of Arabidopsis thaliana and Tobacco...
APPLICATION AREAS
• Coated paper
• Agriculture mulch film
• Shopping bags
• Food waste films and bags
• Consumer packaging...
Reference
Andreas Lössl, Karen Bohmert ,Hans Harloff , Christian Eibl
, Stefan Mühlbauer and Hans-Ulrich Koop (2005).Induc...
Expression of Biodegradable plastics in Plants
Expression of Biodegradable plastics in Plants
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Expression of Biodegradable plastics in Plants

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  •  Polyhydroxyalkanoates (PHAs) like the poly-3-hydroxybutyrate (PHB), polyhydroxyvalerate (PHV) and polyhydroxyhexanoate (PHH);Poly(3-hydroxybutyrate) (PHB) has been studied most extensively and has triggered commercial interest in this class of polymers.
  • InRalstoniaeutropha, the genes for these enzymes are organized in a single operon.
  • TPSS, DNA sequence for the transit peptide of the small subunitof RuBisCoThe aadA cassette used for spectinomycin selection wasexpressed constitutively by the 16S rRNA promoter with the terminationregion from a ChlamydomonasrbcL gene (TrbcLC)
  • Although most work on PHB biosynthesis has been done in the model plant A. thaliana. high accumulation of PHB in commercial crop plants.
  • Expression of Biodegradable plastics in Plants

    1. 1. Expression of biodegradable plastics in plants J.GODWIN BTB-11-010
    2. 2. Introduction Plants can be used as living factories to produce biodegradable plastics from renewable resources. Poly(3-hydroxyalkanoates) are a group of biodegradable structurally simple macromolecules that are synthesized by several microorganisms. The bacterium Ralstonia eutropha can synthesize PHB from glucose and store it as discrete granules to levels as high as 85% of the cell dry weight.
    3. 3. Biosynthesis of PHB
    4. 4. Generating transgenic plants for PHB synthesis Transferring the complete phb operon, consisting of the phbC,phbA and phbB genes from Ralstonia eutropha. The polyester PHB is derived from acetyl-CoA by a sequence of three enzymatic reactions  First condensation of two molecules of acetyl-CoA is catalyzed by β-ketothiolase to form acetoacetyl-CoA.  Acetyl-CoA reductase reduces acetoacetyl-CoA to β- hydroxybutyryl-CoA.  It is polymerized by PHB synthase to PHB.
    5. 5. Problems There is an obvious need to improve transgenic PHB production by producing higher accumulation without side-effects such as  Chlorosis  Male sterility  Growth retardation
    6. 6. Inducible trans-activation in plastids chloroplast transit peptide sequence + ethanol inducible promoter
    7. 7. Expression cassette for nuclear genome and plastid transformation
    8. 8. Achievements Targeting of the polyhydroxybutyrate biosynthetic pathway to the plastids of Arabidopsis thaliana and Tobacco results in high levels of polymer accumulation. Accumulation of PHB up to a concentration of 5% of dry weight has also been produced in the plastids of the hairy roots of sugar beet (Beta vulgaris L.) by nuclear transformation. By targeting PHB synthesis to the plastids of maize and rape seed(Brassica napus). Synthesis of polyhydroxybutyrate in fiber cells of Cotton with enhanced insulating properties.
    9. 9. APPLICATION AREAS • Coated paper • Agriculture mulch film • Shopping bags • Food waste films and bags • Consumer packaging materials etc
    10. 10. Reference Andreas Lössl, Karen Bohmert ,Hans Harloff , Christian Eibl , Stefan Mühlbauer and Hans-Ulrich Koop (2005).Inducible Trans-activation of Plastid Transgenes: Expression of the R.eutropha phb Operon in Transplastomic Tobacco. Plant Cell Physiology. 46(9): 1462–1471. Christiane Nawrath, Yves Poirier, And Chris Somerville(1994).Targeting of the polyhydroxybutyrate biosynthetic pathway to the plastids of Arabidopsis thaliana results in high levels of polymer accumulation. Applied Biological Sciences Vol. 91, pp.12760-12764.

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