Successfully reported this slideshow.
We use your LinkedIn profile and activity data to personalize ads and to show you more relevant ads. You can change your ad preferences anytime.
Why detection of micro
organisms in food is important?
“2.2 million people die annually due
to food and water borne illnes...
Why should We Use Biosensors as an
Alternative?
Less time consuming
Easy to use for on site
detection
No need of extens...
What is a Biosensor???
• A biosensor is an analytical
device, used for the
detection of an analyte,
that combines a biolog...
How does a Biosensor Work?
Analyte
of
Interest
Bioreceptor Transducer Signal
Biosensor
Bacteriophages
• A bacteriophage is a virus that
infects and replicates within a
bacterium cell.
• Specific
• Attacks only...
Bioluminescence
• Production of light by a
chemical reaction within a
living organism.
• Need Luciferase as the
enzyme
• A...
Reaction of Bioluminescence
Simply…
Applications in Food Industry
• A511::lux AB recombinant phage
is used to identify Listeria
bacterium in Spikked ricotta
c...
Limitations
• Presence of specific phage
inhibition antiviral bacterial
immunity system.
• Selecting specific phages.
Future..
• You can check your
food at home..
Bacteriophage based Biosensors
Bacteriophage based Biosensors
Upcoming SlideShare
Loading in …5
×

Bacteriophage based Biosensors

792 views

Published on

Bacteriophage based biosensor is technology which is used for detection of micro organisms. In this presentation it discusses about the potential uses of it in Food Science.

Published in: Science
  • Be the first to comment

Bacteriophage based Biosensors

  1. 1. Why detection of micro organisms in food is important? “2.2 million people die annually due to food and water borne illnesses, and 1.9 millions among them are children” -WHO
  2. 2. Why should We Use Biosensors as an Alternative? Less time consuming Easy to use for on site detection No need of extensive training
  3. 3. What is a Biosensor??? • A biosensor is an analytical device, used for the detection of an analyte, that combines a biological component with a physicochemical detector.
  4. 4. How does a Biosensor Work? Analyte of Interest Bioreceptor Transducer Signal Biosensor
  5. 5. Bacteriophages • A bacteriophage is a virus that infects and replicates within a bacterium cell. • Specific • Attacks only to live cells • Called as “Reporter-phage”
  6. 6. Bioluminescence • Production of light by a chemical reaction within a living organism. • Need Luciferase as the enzyme • And Luferin, photoprotein or a long chain aldehyde as substrate.
  7. 7. Reaction of Bioluminescence
  8. 8. Simply…
  9. 9. Applications in Food Industry • A511::lux AB recombinant phage is used to identify Listeria bacterium in Spikked ricotta cheese, chocolate pudding etc. • A511::lux AB is used to check the quality of minced meat and soft cheese. • P22::lux AB is used to identify Salmonella Spp. • ( ex:- in eggs )
  10. 10. Limitations • Presence of specific phage inhibition antiviral bacterial immunity system. • Selecting specific phages.
  11. 11. Future.. • You can check your food at home..

×