metagenomics

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metagenomics

  1. 1. MetagenomicsMICROBIOLOGY LABORATORY SUBMITTED TO DR. WALEED ALBASHA PREPARED BY: GHAYDA’A SHAQOUR 2012-2013
  2. 2. Introduction Microbes play an enormous role in processes that relate to nearly every aspect of our planet Microbes Maintain the atmosphere Microbes Keep Us Healthy Microbes Clean Up Fuel Leaks
  3. 3. Metagenomics It is both a set of research techniques Meta means “transcendent” “Metagenomics” refers to the collection of genomes of complex microbial communities, and these communities include many different types of microbes
  4. 4. Metagenomics Millions of organisms in one sample Millions of random fragments of DNA It is like jigsaw puzzles
  5. 5. Procedures Environmental Shotgun Sequencing (ESS):A. Sampling from habitatB. Filtering particles, typically by sizeC. Lysis and DNA extractionD. Cloning and library constructionE. Sequencing the clonesF. Sequence assembly into contigs and scaffolds
  6. 6. Procedures High-throughput sequencing:1. Illumina Genome Analyzer II2. Applied Biosystems SOLiD system
  7. 7. Illumina Genome Analyzer II
  8. 8. MetaHIT Human intestinal microbial metagenome Understand the role of microbes in health and in GI disorders such as IBS, obesity and metabolic syndrome.
  9. 9. Potential contributions Earth Sciences Life Sciences Biomedical Sciences Bioenergy Biotechnology Biodefense and Microbial Forensics
  10. 10. Read more The New Science of Metagenomics: Revealing the Secrets of Our Microbial Planetfree PDF downloade http://www.nap.edu/catalog/11902.html Metagenomics of the Human Intestinal Tracthttp://www.metahit.eu
  11. 11. References http://www.cloud- sequencing.com/service/service_76.aspx?typeid=4 http://www.ucc.ie/research/apc/content/research/bacte rial_genomics.html http://mgrc.com.my/new/services-metagenomics.html http://www.interacademies.net/cms/7665.aspx http://www.horizonpress.com/metagenomics http://en.wikipedia.org/wiki/Metagenomics http://www.metahit.eu/index.php?id=410 http://www.mayomedicallaboratories.com/articles/com munique/2010/05.html
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