MetagenomicsMICROBIOLOGY LABORATORY SUBMITTED TO DR. WALEED ALBASHA PREPARED BY: GHAYDA’A SHAQOUR 2012-2013
Introduction Microbes play an enormous role in processes that relate to nearly every aspect of our planet Microbes Maintain the atmosphere Microbes Keep Us Healthy Microbes Clean Up Fuel Leaks
Metagenomics It is both a set of research techniques Meta means “transcendent” “Metagenomics” refers to the collection of genomes of complex microbial communities, and these communities include many different types of microbes
Metagenomics Millions of organisms in one sample Millions of random fragments of DNA It is like jigsaw puzzles
Procedures Environmental Shotgun Sequencing (ESS):A. Sampling from habitatB. Filtering particles, typically by sizeC. Lysis and DNA extractionD. Cloning and library constructionE. Sequencing the clonesF. Sequence assembly into contigs and scaffolds
MetaHIT Human intestinal microbial metagenome Understand the role of microbes in health and in GI disorders such as IBS, obesity and metabolic syndrome.
Potential contributions Earth Sciences Life Sciences Biomedical Sciences Bioenergy Biotechnology Biodefense and Microbial Forensics
Read more The New Science of Metagenomics: Revealing the Secrets of Our Microbial Planetfree PDF downloade http://www.nap.edu/catalog/11902.html Metagenomics of the Human Intestinal Tracthttp://www.metahit.eu