‫بسم ا الرحمن الرحيم‬
GENUS:
CORYNEBACTERIUM
Prof. Khalifa Sifaw Ghenghesh
•
•
•
•
•

Diptheroides = Coryneforms
Gram +ve,
Nonmotile Rods.
Arranged in V forms or Palisades
Catalase +ve
1. Corynebacterium diphtheriae
• Diphtheria.

Morphology
• Pleomorphic Bacilli
• Cells Contain Metachromatic Granules.
Corynebacterium diphtheriae
Club-shaped, Gram-positive rods
Cultural Characteristics
• i. Loeffler's Serum Slope.
• ii. Tellurite BA.
- Temp. 37oC,
- Aerobic and Facultative AnO2.
Corynebacterium diphtheriae forms black
colonies on tellurit agar (left), on blood agar
colonies appear white (right)
PATHOGENICITY
• Diphtheria is a Toxaemia.
– Exotoxin: M. wt 62000
– 2 Fragaments >> A & B
– Inhibits Protein Synthesis.
– ...
LABORATORY DIAGNOSIS
• Important >> Specific Treatment Should Be
Started

Immediately on Suspicion of Diphtheria.

• Speci...
PATHOGENICITY TESTS
• In-vivo Tests:
i. Subcutaneous Test:
ii. Intracutaneuos Test:

• In-vitro Test:
– Elek Test ( Gel Pr...
TREATMENT
• i. Antitoxin: > Moderate Cases:
> Severe Cases:
• ii. Antibiotic:

PREVENTION
• i. Active Immunization >> PDT....
SCHICK TEST
• > Injection of 0.2 ml of Diphtheria Toxin
Intradermally.
• > Positive Reaction >>
Erythema +
Swelling
SCHICK TEST
______________________________________________________________________
Result
Test Arm
Control Arm
Interpretat...
‫بسم ا الرحمن الرحيم‬

Listeria monocytogenes
• Listerosis.
•
•
•
•
•

G+ve Coccobacilli > in Short Chains.
Motile at 25oC But Not at 37oC.
Aerobic to Microaerophilic.
...
• Incidence and Transmission >

DISEASE
• i. In Adults:
• ii. Neonatal Infections:
a. Early Type:
b. Late Type:
LABORATORY DIAGNOSIS
• i. Specimen: Depend on Syndrome: Blood,

CSF, Genital Tract Secretions.

• ii. Media: Selective Med...
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Lecture 7 Corynebacterium and listeria

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Lecture 7 Corynebacterium and listeria

  1. 1. ‫بسم ا الرحمن الرحيم‬ GENUS: CORYNEBACTERIUM Prof. Khalifa Sifaw Ghenghesh
  2. 2. • • • • • Diptheroides = Coryneforms Gram +ve, Nonmotile Rods. Arranged in V forms or Palisades Catalase +ve
  3. 3. 1. Corynebacterium diphtheriae • Diphtheria. Morphology • Pleomorphic Bacilli • Cells Contain Metachromatic Granules.
  4. 4. Corynebacterium diphtheriae Club-shaped, Gram-positive rods
  5. 5. Cultural Characteristics • i. Loeffler's Serum Slope. • ii. Tellurite BA. - Temp. 37oC, - Aerobic and Facultative AnO2.
  6. 6. Corynebacterium diphtheriae forms black colonies on tellurit agar (left), on blood agar colonies appear white (right)
  7. 7. PATHOGENICITY • Diphtheria is a Toxaemia. – Exotoxin: M. wt 62000 – 2 Fragaments >> A & B – Inhibits Protein Synthesis. – Has Special Affinity for Certain Tissues "Mycocardium, Adrenals, Nerve Endings". • Toxin >> Toxoid. • Site of Infection >> Faucial. – Toxin >> Pseudomemebrane.
  8. 8. LABORATORY DIAGNOSIS • Important >> Specific Treatment Should Be Started Immediately on Suspicion of Diphtheria. • Specimen: Two Swabs from the Lesion. • Culture: i. Loeffler's Serum Slope >> Albert Stain. ii. Tellurite BA. iii. BA >> Streptococcal or Staphylococcal Pharyngitis.
  9. 9. PATHOGENICITY TESTS • In-vivo Tests: i. Subcutaneous Test: ii. Intracutaneuos Test: • In-vitro Test: – Elek Test ( Gel Precipitaion Test). • Tissue Culture:
  10. 10. TREATMENT • i. Antitoxin: > Moderate Cases: > Severe Cases: • ii. Antibiotic: PREVENTION • i. Active Immunization >> PDT. • ii. Passive Immunization: • iii. Combined:
  11. 11. SCHICK TEST • > Injection of 0.2 ml of Diphtheria Toxin Intradermally. • > Positive Reaction >> Erythema + Swelling
  12. 12. SCHICK TEST ______________________________________________________________________ Result Test Arm Control Arm Interpretation Diphthri (Toxin) Inactivated Toxin Immunization 36h 120h 36h 120h ______________________________________________________________________ Negative ----Immune, Not Not Hypersensitive Required --------------------------------------------------------------------------------------Positive + + -- -- Not Immune, Not Required Hypersensitive --------------------------------------------------------------------------------------------------------------------Negative + -+ -Immune Not Hypersensitive Required --------------------------------------------------------------------------------------------------------------------Positive & + + + -Not Immune ContraPseudo Hypersensitive indicated ______________________________________________________________________
  13. 13. ‫بسم ا الرحمن الرحيم‬ Listeria monocytogenes
  14. 14. • Listerosis. • • • • • G+ve Coccobacilli > in Short Chains. Motile at 25oC But Not at 37oC. Aerobic to Microaerophilic. On BA > Narrow Zone of β-Haemolysis. Listerolysin O >
  15. 15. • Incidence and Transmission > DISEASE • i. In Adults: • ii. Neonatal Infections: a. Early Type: b. Late Type:
  16. 16. LABORATORY DIAGNOSIS • i. Specimen: Depend on Syndrome: Blood, CSF, Genital Tract Secretions. • ii. Media: Selective Media. TREATMENT • Penicillin, Ampicillin.

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