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Lecture 21 Vibrio cholerae

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Lecture 21 Vibrio cholerae

  1. 1. ‫بسم ا الرحمن الرحيم‬ FAMILY: VIBRIONACEAE Prof. Khalifa Sifaw Ghenghesh
  2. 2. 3 Important Genera: • Vibrio • Aeromonas, • Plesiomonas • Oxidase +ve Habitat:
  3. 3. ‫بسم ا الرحمن الرحيم‬ Vibrio cholerae I. V. cholera O1: • Agglutinate in Antisera Directed Against O1 Antigen. • Produce Enterotoxin. 2. Non-Toxigenic V. cholera O1: 3. Non O1 V. cholera (NCV, NAV): II. V. cholerae O139
  4. 4. Vibrio cholerae Gram-negative, curved rods
  5. 5. Vibrio cholerae Leifson flagella stain
  6. 6. Vibrio cholerae curved rods with polar flagellum (EM)
  7. 7. V. cholerae O1 --------------------------------------------------Biotye Haemolysis (SRBC) VP El-tor + Classic -- + -- Resistance to PB + ----------------------------------------------------
  8. 8. VIRULENCE FACTORS: • Enterotoxin: CT • Adherence: Ability to Attach to the Microvilli at the Brush Borders of the Epithelial Cells. EPIDEMIOLOGY: • Causes Widespread Disease. • Human Carriers: • Modern International Travel:
  9. 9. PATHOGENESIS & CLINICAL INFECTION: • • • • • Infectious Dose: Incubation period: Abrupt Onset of Diarrhoea & Vomiting Stool > Watery (Rice Water), Odorless Fatality Rate:
  10. 10. LAB. DIAGNOSIS: • Isolation & Identification of the Organism. • Media: TCBS • Transport Media: TREATMENT: • Prompt Replacement of Fluid & Elecrolytes • Tetracycline and others PREVENTION & CONTROL: • Adequate Sewage Treatment and Water Purification Systems. • Vaccination: ??
  11. 11. Typical Vibrio cholera contaminated water supply
  12. 12. Vibrio parahaemolyticus • Halophilic vibrio • Associated with seafood • Gastroenteritis: – Outbreaks of Food Poisoning • Extraintestinal infections > wounds • Virulence Factors: – Haemolysin > – heat-stable cytotoxin – Heat-labile enterotoxin – Adherence to human intestinal cells
  13. 13. Aeromonas species A. hydrophila, A. sobria & A. caviae • Enterotoxins, Cytotoxins, Haemolysins, Etc… • Gastroenteritis, Septicaemia, and Skin & Wound Infections TREATMENT • TMP-SMX, Tetracycline, Gentamicin • Resistant to β-Lactam Antibiotics.

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