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Presented by: Mike McKenna
Setting Up Your Cannabis Lab
for Potency Testing
• Cannabichromene (CBC)
• Cannabidiol (CBD)
• delta-8-THC
• delta-9-THC
• Cannabigerol (CBG)
• Cannabinol (CBN)
Analysis o...
Three Stages of Cannabis Analysis:
1. Extraction
2. Analysis
3. Reporting
Discussion Points:
GenTechScientific.com Booth #...
Sample must be put into liquid phase.
• Step 1: Representative Sampling of Plant
• Step 2: Sieving Plant Material
• Step 3...
• Cuttings:
▫ Taken from all over the plant
▫ Composited together
Extraction Step 1: Representative Sampling of Plant
Sour...
• Sieving:
▫ Cuttings are placed in a sieve
▫ Shaken until a sized
proportion is collected.
Extraction Step 2: Sieving Pla...
• Extraction
▫ Weigh plant material into a vial
or beaker
▫ Add solvent
▫ CO2 extraction
Extraction Step 3: Extraction of ...
• Gas Chromatography Flame Ionization Detector (GC/FID)
• Gas Chromatography Mass Spectrometry (GC/MS)
• High Performance ...
Gas Chromatographs are used for the
Cannabis testing applications, such as for
Residual Solvents, Cannabinoid
Characteriza...
Same procedure as Gas Chromatography
Flame Ionization but ….
• molecules are detected by mass and
mass fragmentation
• Mos...
Along with a GC, an HPLC can assist in
quantifying the potency of a Cannabis
sample. It's possible to accurately determine...
When testing for residual solvents such
as pesticides, water, butane, carbon
dioxide, ethanol and other solvents used
in c...
Chromatograms that are created are integrated by several kinds of devices.
• Integrator
• Chromatography Data Stations
• L...
• Cheap
• Labor-intensive
• Transcribe results into spreadsheet
• Integrator and mathematically corrected results are tran...
• Chromatograms are processed by
computer software
• Reports are generated with
identification of analytes and
concentrati...
• Results are transferred to a computer
server where they are manipulated and
placed on a final customer report
• Least la...
Training
• We offer customized hands-on training at our location or yours!
• Train your team on testing theory, operation ...
• Extraction of Marijuana Plant Material
▫ Representative Sampling, Sieving Plant, Extraction
• Analysis of Extracts
▫ GC/...
References: Restek, Marijuana Growers Headquarters & Analytical 360
Thank You
from
Booth #105
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Setting Up Your Cannabis Lab for Potency Testing

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Setting up a cannabis lab can be overwhelming, in addition to compliance there are many concerns including equipment, personnel, location, etc. First presented at the Cannabis World Congress & Business Exposition in New York City by Michael McKenna, Chemist/Service Engineer, GenTech Scientific, Inc. June 19, 2015

The presentation covers topics such as Analysis of Cannabidiols in Marijuana plants, including extraction, analysis and reporting.

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Setting Up Your Cannabis Lab for Potency Testing

  1. 1. Presented by: Mike McKenna Setting Up Your Cannabis Lab for Potency Testing
  2. 2. • Cannabichromene (CBC) • Cannabidiol (CBD) • delta-8-THC • delta-9-THC • Cannabigerol (CBG) • Cannabinol (CBN) Analysis of Cannabidiols in Marijuana Plants: Fig 1: Cannabinoid-Structures Source: http://blog.restek.com/?p=7704
  3. 3. Three Stages of Cannabis Analysis: 1. Extraction 2. Analysis 3. Reporting Discussion Points: GenTechScientific.com Booth #105 Instrumentation - Training - Service
  4. 4. Sample must be put into liquid phase. • Step 1: Representative Sampling of Plant • Step 2: Sieving Plant Material • Step 3: Extraction or Sieved Plant Material Extraction of Marijuana Plant Material GenTechScientific.com Booth #105 Instrumentation - Training - Service
  5. 5. • Cuttings: ▫ Taken from all over the plant ▫ Composited together Extraction Step 1: Representative Sampling of Plant Source: www.oregonlive.com GenTechScientific.com Booth #105 Instrumentation - Training - Service
  6. 6. • Sieving: ▫ Cuttings are placed in a sieve ▫ Shaken until a sized proportion is collected. Extraction Step 2: Sieving Plant Material Source: www.allbud.com GenTechScientific.com Booth #105 Instrumentation - Training - Service
  7. 7. • Extraction ▫ Weigh plant material into a vial or beaker ▫ Add solvent ▫ CO2 extraction Extraction Step 3: Extraction of Sieved Plant Material GenTechScientific.com Booth #105 Instrumentation - Training - Service
  8. 8. • Gas Chromatography Flame Ionization Detector (GC/FID) • Gas Chromatography Mass Spectrometry (GC/MS) • High Performance Liquid Chromotography (HPLC) • Liquid Chromatography Mass Spectrometry (LC/MS) Analysis of Extracts GenTechScientific.com Booth #105 Instrumentation - Training - Service
  9. 9. Gas Chromatographs are used for the Cannabis testing applications, such as for Residual Solvents, Cannabinoid Characterization and THC potency. • Place aliquot of extract into a heated injection port • Molecules in the gas phase are separated by partitioning between the gas phase and the stationary phase • Detected by ionization in the flame detector Gas Chromatography (GC/FID) Fig 2: FID Source: http://blog.restek.com/?p=7704 GenTechScientific.com Booth #105 Instrumentation - Training - Service
  10. 10. Same procedure as Gas Chromatography Flame Ionization but …. • molecules are detected by mass and mass fragmentation • Most reliable method of identification Gas Chromatography/ Mass Spectrometry (GC/MS) Agilent 5973N GC/MSD System with 6890 GC and 7683 Autosampler GenTechScientific.com Booth #105 Instrumentation - Training - Service
  11. 11. Along with a GC, an HPLC can assist in quantifying the potency of a Cannabis sample. It's possible to accurately determine the levels of the components such as THC, THCA, CBD, CDBA and CBN. • Molecules partitioned between liquid phase and solid support coated with polymer • Molecules are not degraded by heat High Pressure Liquid Chromatography (HPLC) Waters 2695 Separations Module with 2487 Detector GenTechScientific.com Booth #105 Instrumentation - Training - Service
  12. 12. When testing for residual solvents such as pesticides, water, butane, carbon dioxide, ethanol and other solvents used in cannabis manufacturing, an LCMS will assist in determining their levels. • Same procedure as liquid chromatography but molecules are detected by mass fragmentation • Solvent Residue • Pesticide Residue Liquid Chromatography/ Mass Spectrometry (LC/MS) AB Sciex API 3200 QTRAP® LC/MS/MS GenTechScientific.com Booth #105 Instrumentation - Training - Service
  13. 13. Chromatograms that are created are integrated by several kinds of devices. • Integrator • Chromatography Data Stations • LIMS Systems Reporting of Results of Extracts GenTechScientific.com Booth #105 Instrumentation - Training - Service Source: http://blog.restek.com/?p=7704
  14. 14. • Cheap • Labor-intensive • Transcribe results into spreadsheet • Integrator and mathematically corrected results are transferred to final client report. Integrator GenTechScientific.com Booth #105 Instrumentation - Training - Service
  15. 15. • Chromatograms are processed by computer software • Reports are generated with identification of analytes and concentrations • Results are then added to a final customer report Chromatography Data Stations GenTechScientific.com Booth #105 Instrumentation - Training - Service
  16. 16. • Results are transferred to a computer server where they are manipulated and placed on a final customer report • Least labor involved GenTechScientific.com Booth #105 Instrumentation - Training - Service Laboratory Information Management Systems
  17. 17. Training • We offer customized hands-on training at our location or yours! • Train your team on testing theory, operation of the instrument, how to effectively run samples, and method development. GenTech Solutions: GenTechScientific.com Booth #105 Instrumentation - Training - Service Installation • GenTech offers the option to install and setup your lab. • Also includes operator familiarization training from our expert service engineer.
  18. 18. • Extraction of Marijuana Plant Material ▫ Representative Sampling, Sieving Plant, Extraction • Analysis of Extracts ▫ GC/FID, GC/MS, HPLC, LC/MS Instrumentation • Reporting of Results of Extracts ▫ Integrator, Chromatography Data Solutions, LIMS Systems • GenTech Services: ▫ Training & Installation Summary GenTechScientific.com Booth #105 Instrumentation - Training - Service
  19. 19. References: Restek, Marijuana Growers Headquarters & Analytical 360 Thank You from Booth #105

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