Strengthening Rice BreedingProgram in Mekong RegionTheerayut ToojindaJonaliza Lanceras-SiangliwGCP General Research Meetin...
Rice Planting Area in Mekong RegionRice PlantingArea (millionha)Thailand Myanmar Laos CambodiaIrrigated 1.84 1.62 0.07 0.2...
Major Constraints in Rice Production in theMekong RegionSubmergence : In 2006 0.8 million ha wereflooded (500 million $)Br...
Goals of Mekong Region in Rice Improvement Improve grain eating and cooking quality Stabilize yield of Mekong mega varie...
Rice Gene Discovery Unit – A Research Hub Genetic Information QTL/Gene Information Blast BLB Bph Submergence Salini...
Rice Gene Discovery Unit – A Research HubGenotyping Capacity
Rice Gene Discovery Unit – A Research HubPhenotyping CapacityGreen house
Phase I: Regional Workshop on Molecular Breeding inRice for Mekong Region: Line Conversion Using MAS Funding Agency: Rock...
DAR – Manawthukha (HY) + aroma and intermediate AC- Sin Thwe Latt (HY) + salinity toleranceNAFRI – Thaddokham 1 (HY) + aro...
Phase I: Regional Workshop on Molecular Breeding inRice for Mekong Region: Line Conversion Using MASFirst workshop – June ...
Phase II: Community of Practice “Concept Applied to RiceProduction in Mekong Region: Quick Conversion of PopularRice Varie...
Phase II: Community of Practice “Concept Applied to Rice Production inMekong Region: Quick Conversion of Popular Rice Vari...
Knowledge and Technology Transfer - Workshop On SiteCARDIJune 19-20, 2007DARAugust 20-21, 2007NAFRIJuly 3-4, 2007Video of ...
Aromatic CAR3 CAR3Half kilo of aromatic CAR3 was distributed to 6 farmersfor wet season 2011 farmers’ field trials.
Savannakhet Province Champasak ProvinceAromatic Thadokkam1
12 ha. Aungzeya, Nay Pyi TawPyiamana, Nay Pyi TawLatpadanKyaukseAromatic MNTSalTol STLSalTol STL KyaukseAromatic MNT SalTo...
Sagaing Mandalay Yangon Bago (East) Ayeyarwaddy Rakh ine AVG Range1 Yn3018-183-1-3-2 4.01 5.23 3.88 4.05 2.90 4.76 4.14 22...
Yield performance of On- Farm trials(2010 WS)No Entry NameYield (tha-1)Mean(tha-1)Mandalay AyeyarwaddyTaninthari1 Yn 3220M...
Ms. Myint Yi (DAR)Mr. Then Rathmuny (CARDI)
Phase IIIa: Strengthening Human Resource inMekong Region: Community of Practice for RiceBreeding with Emphasis on MAS Fun...
Rice Breeding Program of NAFRI (Laos) and CARDI (Cambodia)Season2010 WS2011 WS2012 DSxMASAromaticTDK1 withSubIR85264-34-14...
Rice Breeding Program of DAR, MyanmarSeason2009 WS2010DS2010 WS2011DS2011WS2012DSxxMASAromatic MNT(BC4F4-75)RGD07097-1-8M-...
Rice Breeding Program of DAR, MyanmarSeason2009 WS2010DS2010 WS2011DS2011WS2012DSMASPaw San Mhwe(Aromatic)IR 57514-333, -3...
Rice Breeding Program of ThailandKD571-77 x IR57514F1 x IR57514BC1F1 x IR57514BC2F1BC3F1MASBC3F1UBNMASBC3F2BC3F3Field Test...
Phase IIIb: The Community of Practice “Strengthening RiceBreeding Program using Genotyping Building Strategy andImproving ...
Facilities for Trait PhenotypingGreenhouse for BBscreening andsubmergence pondsin DAR
Submergence ponds and BPH facilities in CARDI
Submergence ponds and greenhouse for blastscreening in NAFRI
Knowledge and Technology Transfer - Workshop On SiteVideo of phenotyping protocols1. Experimental designs and analysis2. S...
AcknowledgementU Khin SoeTin Tin MyintKhin Myo WinThidaKyaw Swar OoBounthoung BouahomPhoumi IthapanyaKongpanh KanyavongPhe...
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GRM 2011: Strengthening rice breeding in the Mekong -- A community of practice

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GRM 2011: Strengthening rice breeding in the Mekong -- A community of practice

  1. 1. Strengthening Rice BreedingProgram in Mekong RegionTheerayut ToojindaJonaliza Lanceras-SiangliwGCP General Research MeetingHyderabad, IndiaSept. 21-25, 2011
  2. 2. Rice Planting Area in Mekong RegionRice PlantingArea (millionha)Thailand Myanmar Laos CambodiaIrrigated 1.84 1.62 0.07 0.20RainfedLowland7.36 3.89 0.49 2.15RainfedUpland0.01 1.46 0.10 0.05Submergenceprone0.18 1.13 0.00 0.10Total 9.20 8.10 0.66 2.50
  3. 3. Major Constraints in Rice Production in theMekong RegionSubmergence : In 2006 0.8 million ha wereflooded (500 million $)Brown plant hopper : In 2010, BPH caused theyield loss up to 1.1 million tons (350 million $)Drought :In 2004, more than 1.2 million ha of the riceproduction areas were hit by drought causing theyield loss for 1.83 million ton (370 million $)Blast : In 2007, blast disease infected rainfedlowland rice causing the yield loss 50-85 %.
  4. 4. Goals of Mekong Region in Rice Improvement Improve grain eating and cooking quality Stabilize yield of Mekong mega varieties byimproving resistance against biotic and abioticstresses Strengthen human resource in the region byapplying MAS biotechnology in rice breeding Improve phenotyping facilities in rice breeding
  5. 5. Rice Gene Discovery Unit – A Research Hub Genetic Information QTL/Gene Information Blast BLB Bph Submergence Salinity Drought Rice Quality Marker Technology SSR SNP Microarray
  6. 6. Rice Gene Discovery Unit – A Research HubGenotyping Capacity
  7. 7. Rice Gene Discovery Unit – A Research HubPhenotyping CapacityGreen house
  8. 8. Phase I: Regional Workshop on Molecular Breeding inRice for Mekong Region: Line Conversion Using MAS Funding Agency: Rockefeller Foundation, BIOTEC,Kasetsart University Duration: 2004 – 2006 Objectives: Transfer DNA technology and genomic information fromRGDU to breeding institutes in the region Improvement of traditional cultivars by using MAS Enhance research collaboration between/among institutesin countries in Mekong region
  9. 9. DAR – Manawthukha (HY) + aroma and intermediate AC- Sin Thwe Latt (HY) + salinity toleranceNAFRI – Thaddokham 1 (HY) + aroma and low GTCARDI – CAR3 (DT) + aroma and low GTTHAILAND – IR57514 (DT, Sub) + aroma and low AC- RD6 (quality) + blast resistance- Chainat1 (HY) + bph resistance
  10. 10. Phase I: Regional Workshop on Molecular Breeding inRice for Mekong Region: Line Conversion Using MASFirst workshop – June 2004Second workshop – January 2005Third workshop – June 2005Fourth workshop – January 2006
  11. 11. Phase II: Community of Practice “Concept Applied to RiceProduction in Mekong Region: Quick Conversion of PopularRice Varieties with Emphasis on Drought, Salinity and GrainQuality Improvement” Funding Agencies: Generation Challenge Program;BIOTEC Duration: 2007 – 2009 Objectives: Continuation of line conversion using MAS Validation of introgression lines for target traitperformance, yield and agronomic characters Workshop on QTL and marker-assisted selection for plantbreeding
  12. 12. Phase II: Community of Practice “Concept Applied to Rice Production inMekong Region: Quick Conversion of Popular Rice Varieties withEmphasis on Drought, Salinity and Grain Quality Improvement”First workshop – May 2007Second workshop – November 2007Third workshop – May 2008Fourth workshop – April 2009
  13. 13. Knowledge and Technology Transfer - Workshop On SiteCARDIJune 19-20, 2007DARAugust 20-21, 2007NAFRIJuly 3-4, 2007Video of labprotocols forMAS
  14. 14. Aromatic CAR3 CAR3Half kilo of aromatic CAR3 was distributed to 6 farmersfor wet season 2011 farmers’ field trials.
  15. 15. Savannakhet Province Champasak ProvinceAromatic Thadokkam1
  16. 16. 12 ha. Aungzeya, Nay Pyi TawPyiamana, Nay Pyi TawLatpadanKyaukseAromatic MNTSalTol STLSalTol STL KyaukseAromatic MNT SalTol STLAromatic IR57514
  17. 17. Sagaing Mandalay Yangon Bago (East) Ayeyarwaddy Rakh ine AVG Range1 Yn3018-183-1-3-2 4.01 5.23 3.88 4.05 2.90 4.76 4.14 22 Yn2684-2-5-1-2-1 4.31 5.48 3.43 3.60 2.81 4.03 3.94 53 Yn2744-49-383-2-3 4.19 5.05 4.12 3.21 3.45 3.75 3.96 44 Yn3248-MAS-BC4F2-1124.41 5.38 3.49 3.62 5.42 3.34 4.28 15 Manawthukha 4.51 4.52 4.26 4.05 4.81 2.38 4.09 3Mean Yield 4.26 5.13 3.84 3.70 3.88 3.65 4.08Sr. no. EntriesGrain yield (Ton/ha)Yield performance on different locationsMaturity Days - 137Plant Height (cm) - 103-117Productive tillers - 9 - 11Total grains / panicle - 90 - 117Fill grain (%) - 88 – 951000 grain weight (gm) - 21.4Yield (Bsk/ac) - 83 - 106Group - (B)Milling (%) - 54Amylose content (%) - 25.8Eating quality - goodGrain quality - Trans;Distinct character - smellAdapted locations -Both of Irrigated and Rainfed lowlandYn 3248-BC4F2-112
  18. 18. Yield performance of On- Farm trials(2010 WS)No Entry NameYield (tha-1)Mean(tha-1)Mandalay AyeyarwaddyTaninthari1 Yn 3220MAS 108-2-3-1 2.5 3.9 2.8 3.12 Yn 3220MAS 77-2-9-2 2.5 4.1 3.8 3.53 Yn 3220MAS 62-2-4 1.7 3.9 2.6 2.74 Yn 3220MAS 76-4-1 3.5 4.5 2.5 3.55 Sin Thwe Latt 2.6 3.8 2.9 3.1Mean 2.6 4.0 2.9Maturity Days - 150Plant Height (cm) - 93.8Productive tillers - 5 - 7Total grains / panicle - 126Fill grain (%) - 741000 grain weight (gm) - 32Yield (tha-1) - 4.0 -4.5Varietal Group - EmataMilling (%) - 51.3Amylose content (%) - 20.7Eating quality - goodGrain quality - Translucence, wbDistinct character - tolerance to EC 6 dS/mAdapted locations -Costal and Inland salinity areasYn 3220-MAS-76-4-1
  19. 19. Ms. Myint Yi (DAR)Mr. Then Rathmuny (CARDI)
  20. 20. Phase IIIa: Strengthening Human Resource inMekong Region: Community of Practice for RiceBreeding with Emphasis on MAS Funding Agency: BIOTEC, Kasetsart University,Rajamongkol University of Technology Lanna,Ubon Ratchatani University Duration: 2009 – 2013 Objectives: Develop human resource by providing scholarshipto staffs of DAR, NAFRI, CARDI and UBU byimproving popular rice variety using MAS
  21. 21. Rice Breeding Program of NAFRI (Laos) and CARDI (Cambodia)Season2010 WS2011 WS2012 DSxMASAromaticTDK1 withSubIR85264-34-141(Sub, Xa21, BL)XAromaticTDK1X10 F11500 F2F3Plant type selectionScreening for blastand submergencetoleranceAromaticTDK1TDK1 (Sub)X 2010 DSSeason2010 WS2011 DS2011 WSxAromaticCAR3IR64 (Sub)XAromaticCAR3X10 F1BC1F1ScreenSubBC1F2XBC2F2Screen Suband BphxF1F2Select planttype2012 DSBPHdonorXxF32012 WS2013 DS
  22. 22. Rice Breeding Program of DAR, MyanmarSeason2009 WS2010DS2010 WS2011DS2011WS2012DSxxMASAromatic MNT(BC4F4-75)RGD07097-1-8M-9KPS(xa5, Xa21, xa33)XAromaticMNTX5 F192 BC1F1AromaticMNTX5 selected BC1F1311 BC2F1 MASAromaticMNTX7 selected BC2F1171 BC3F1 MAS1000 BC3F2Plant type selectionand MASBC3F3Screening for BB withThai and MyanmarisolatesSeason2012 WS2012 DS2010 WS2011 DS2011 WS2012 DSxxMASSalTol STL IR57514(Sub, Xa21)XSalTol STL X11 F1298 BC1F1SalTol STL X6 selected BC1F1BC2F1 MASSalTol STL Xselected BC2F1BC3F1 MASBC3F2Plant type selectionand MASBC3F3Screening for salinity andsubmergence tolerance2013 WS2013 DS
  23. 23. Rice Breeding Program of DAR, MyanmarSeason2009 WS2010DS2010 WS2011DS2011WS2012DSMASPaw San Mhwe(Aromatic)IR 57514-333, -309(Sub, aroma, Xa21)XPSM X 38 F1279 BC1F1PSM X 15 selected BC1F1BC2F1 MASBC2F2BC2F3 Screening and seedincreaseBC2F4ObservationYield trialxMAS and plant typeselectionxx2012WS
  24. 24. Rice Breeding Program of ThailandKD571-77 x IR57514F1 x IR57514BC1F1 x IR57514BC2F1BC3F1MASBC3F1UBNMASBC3F2BC3F3Field Testing of BC3F4 in RGDUMASNov. 2007May. 2008Oct. 2008-2009May. 2007MASMASSubmergence screeningGrain quality testRF, BIOTEC, KU2004-2006GCP, BIOTEC2007-2009Multi-location trials andfarmers’ participation inselectionBIOTEC, GCP, RMUTL,KU, UBU, RGDU2009-2013BC3F4-aromatic IR57514 x RD6Glutinous Aromatic IR57514BIOTEC, GCP, RMUTL,KU, UBU, RGDU2009-2013
  25. 25. Phase IIIb: The Community of Practice “Strengthening RiceBreeding Program using Genotyping Building Strategy andImproving Phenotyping Capacity for Biotic and AbioticStresses in Mekong Region” Funding Agency: GCP, BIOTEC Duration: 2009 – 2012 Objectives: Build phenotyping facilities for abiotic and biotic traitsin DAR, NAFRI and CARDI Validate and standardize phenotyping protocol thatfits the condition in each country Conduct phenotyping workshop Asses trait performance through field trials underresearch farm and farmer’s field conditions
  26. 26. Facilities for Trait PhenotypingGreenhouse for BBscreening andsubmergence pondsin DAR
  27. 27. Submergence ponds and BPH facilities in CARDI
  28. 28. Submergence ponds and greenhouse for blastscreening in NAFRI
  29. 29. Knowledge and Technology Transfer - Workshop On SiteVideo of phenotyping protocols1. Experimental designs and analysis2. Screening techniques
  30. 30. AcknowledgementU Khin SoeTin Tin MyintKhin Myo WinThidaKyaw Swar OoBounthoung BouahomPhoumi IthapanyaKongpanh KanyavongPhetmanyseng XangsayasanePhatsalakhone ManivongOuk MakaraSakhan SophanyOuk Sothea

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