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Lactic Acid Bacteria Serum (LABS)

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Research Project for Crop Production(CRSC 2) made by CvSU BSAgriculture 2-2. T
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Lactic Acid Bacteria Serum (LABS)

  1. 1. MARIEL O. PADERNA MAIDEELYN ANILLO BRIAN ANGELO R. SUSTRINA NELO G. PAIRAT RAYMOND JOHNRICK M. DE JESUS MARCO FELIX S. VALDEZ CRSC 2- PRACTICES IN CROP PRODUCTION BS AGRICULTURE 2-2 September 29, 2016
  2. 2. INTRODUCTION Lactic acid bacteria (LAB) are widespread microorganisms which can be found in any environment rich mainly in carbohydrates, LAB are anaerobic microorganisms that decompose sugar in the absence of oxygen. Normally, they are separated and cultured with rice washed water and milk. This is how Lactic Acid Bacteria Serum (LABS) is obtained. Lactic Acid Bacteria Serum is now used for its ability to convert waste into organic matter and basic materials. And they thrive and feed on the ammonia released in the decomposition normally associated with the foul odor (removes foul odor). Lastly, they serves as defense against pathogenic diseases such as harmful viruses and fungi. Today LABS application can be observe on field of natural farming, livestock production, aquaculture, and even in households. OBJECTIVES 1. To produce homemade LABS using inexpensive basic materials. 2. To identify its different use and applications. 3. To make recommendations to improve the production and to maximize its effectivity.
  3. 3. Figure 1 Adding 700 ml water to 1 kg of rice Figure 2 Mixing water and rice Figure 3 Filtering the mixture METHODOLOGY MATERIALS TO BE USED: BOUGHT IN MARKET: OTHERS: 1kg Rice - ₱ 37.00 Water 1 Kg of Molasses - ₱ 9.00 Jar or Container Plastic Basin 1 kg of Powdered Milk - ₱50.00 Strainer (or Clean cloth) TOTAL Cost of Production = ₱96.00 Weigh Scale (or other measuring types) Funnel Paper and Rubber band PROCEDURE W/ PICTURE STEP 1: Put 700 ml of water to 1 kg rice then by use of hand stir, swirl and crush the mixture until milky colored water is attained. (The water is now a rich source of carbohydrates) Obtain rice wash by filtering the mixture using clean cloth.
  4. 4. Figure 4 Covering the jar with paper STEP 2: Place 500 ml rice wash to a jar and cover it with paper (use rubber band to tie the paper covering). Leave the jar in cool dark place for 5-7 days. STEP 3: After a week, when the bran has risen and it smells a little sour and forms 3 layers. This is indicating the rice wash is infected with various microbes. Take about 100 ml of clear water by straining again the mixture. STEP 4: Add 1 L of milk (to obtain 1:10 Water and Milk ratio). You may use skim or powdered milk, although fresh milk is the best. Cover the jar with paper same as before. Leave for 5-7 days in cool dark place. STEP 5: After about 1 week you’ll see curds (made of carbohydrate, protein, and fat) or the white part and yellow liquid or whey, enriched with lactic acid bacteria from the fermentation of the milk. Extract the whey by pouring through a strainer
  5. 5. Figure 6 Adding molasses Figure 7 Finished Product STEP 6: Add Molasses or brown sugar in equal part to the collected whey. This is to preserve the LABS at room temperature. So, if you have 1L of serum, add 1kilo sugar or 1L molasses. Otherwise store in fridge to keep. Then place it in clean container and label it accordingly USES AND APPLICATION OF LABS Based on our research we summarizes the different uses of LABS: It can be used as alternative strategy to antibiotics growth promoters used in farm animals. Because continuous use of antibiotics may increase bacterial resistance, which can threaten the health of both animals and humans. Mix 2tbsp to 1L water, then add that mixture to animal’s water at 2tbsp/L (so the animal’s water contains little less than a quarter tsp/L of lacto serum). But this is very flexible. The Lacto serum is not harmful, so it’s just about adding enough to be effective, without wasting it. Figure 5 The yellow liquid is the whey
  6. 6. In plants, spraying diluted solution of lactic acid bacteria serum (2tbsp. /1 liter of water) to the plant and soil helps plant growth and makes them healthier. As it is applied to the soil or the leaves, these beneficial bacteria aid in the decomposition process, thus allowing more food to be available and assimilated by the plant. And it is also known to produce enzymes and natural antibiotics aiding effective digestion and has antibacterial properties that serves as defense against fungi and viruses. In Aquaculture, with regular addition of this beneficial organism to poor quality water (because of high amount of ammonia that pollutes water) ammonia problem is minimized and helps hasten or complete the denitrification or converting wastes into forms not harmful to fish. Add lacto at roughly 1L per 700m3 of fish-containing water. Other applications: Used for its odor killing ability in animal wastes, clogged drains, foul odor of canals and addition to beneficial organism in composts. RECCOMENDATION We recommend that: 1. Use LABS in morning, to avoid exposure to direct sunlight with UV that may kill or reduce their effectivity. 2. Avoid store milk that have antibiotic components. 3. During production and storage keep away the container from rainwater for this may cause contamination. Also keep it safe from wild animals. 4. Do not use plastic lids or covering that will prevent aeration. That’s why we use paper.
  7. 7. 5. Use gloves and protective gears, aside from LAB it is possible that it contains harmfull organism that may enter your body through exposed body parts (ex. wounds) CONCLUSION Lactic acid bacteria serum are therefore excellent material that can be made only indoors using inexpensive common materials. Modern agriculture must been using this because as what has been stated it can be substituted for antibiotics for animal growers and beneficial for plants that may increase income of farmers using it and other useful applications. REFERENCES Ikeda, David M. et al. Sustainable Agriculture, “Farming: Lactic Acid Bacteria”. August 2013, CTHR. Minoa, Hawaii Gilliland, S. E., 1990. Health and nutritional benefits from lactic acid bacteria. FEMS Microbiol Rev. 1990 Sep;7(1-2):175-88. Lim, A. K., 2005. Handout on natural farming system and technology seminar. Davao: Tribal Mission Foundation International, Inc. Guolong Zhang, The Use of Lactic Acid Bacteria as a Probiotic in Swine Diets, Mar. 2015 URL: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4384071/ Rory Turnbull, DIY Lacto Bacillus Culture, Jan. 2013 URL: https://permies.com/t/25812/permaculture/DIY-Lacto-Bacillus-Culture Gil and Patrick, 2016 URL: http://theunconventionalfarmer.com/recipes/lactobacillus-serum/ Dugas, J., n.d. Lactic acid bacteria. URL: http://www.waksmanfoundation.org/labs/mbl/lactic.html.

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