Taxonomical, Ecophysiological andTaxonomical, Ecophysiological and
Phytochemical StudiesPhytochemical Studies
onon
Solanum...
Supervised bySupervised by
ProfProf.. Mohamed GMohamed G.. Sheded ProfSheded Prof.. Arafa I. HamedArafa I. Hamed
Prof. of ...
Part 1Part 1
Solanum diphyllumSolanum diphyllum L.L.
Local DistributionLocal Distribution
Shoot
Another shoot
A B
Vertical main root
Underground lateral
connection
Shoot
Underground lateral
connection (root later...
A)A) Solanum diphyllumSolanum diphyllum L. Middle-Aged Root. B) Old RootL. Middle-Aged Root. B) Old Root
(Bar = 0.05 mm(Ba...
B
C
D
E
F
A
Solanum diphyllumSolanum diphyllum L. StemL. Stem
Solanum diphyllumSolanum diphyllum L. TrichomesL. Trichomes
A
B
Solanum diphyllumSolanum diphyllum L. LeafL. Leaf
A
C D
B
Solanum diphyllumSolanum diphyllum L. PetioleL. Petiole
Analysis of Different MetabolitesAnalysis of Different Metabolites
Solanum diphyllumSolanum diphyllum L. StomataL. Stomata
A B
Solanum diphyllumSolanum diphyllum L.L. Pollen GrainPollen Grain
Solanum diphyllumSolanum diphyllum L. SeedL. Seed
In vitroIn vitro Cytotoxicity TestCytotoxicity Test
Using SBR AssayUsing SBR Assay
Root
Shoot
Fruit
PhytochemicalPhytochemical
11
H-NMR Spectrum of CompoundH-NMR Spectrum of Compound 11 (600 MHz(600 MHz(.(.
1313
C-NMR Spectrum of CompoundC-NMR Spectrum of Compound 11 (100 MHz(100 MHz(.(.
Structure of CompoundStructure of Compound 11..
(3 – O - {[α - L -rhamnopyranosyl-(1 4)]-[α - L -rhamnopyranosyl- (1 2)]- ...
11
H-NMR Spectrum of CompoundH-NMR Spectrum of Compound 22 (600 MHz(600 MHz(.(.
1313
C-NMR Spectrum of CompoundC-NMR Spectrum of Compound 22 (100 MHz(100 MHz(.(.
Structure of CompoundStructure of Compound 22..
(3 – O - {[α - L -rhamnopyranosyl-(1 4)]-[α - L -rhamnopyranosyl- (1 2)]- ...
Full Mass Spectra for Compound 3 and 4 Using ESIFull Mass Spectra for Compound 3 and 4 Using ESI
(Electro Spray Ionization...
11
H-NMR Spectrum of CompoundH-NMR Spectrum of Compound 33 (500 MHz(500 MHz(.(.
1313
C-NMR Spectrum of CompoundC-NMR Spectrum of Compound 33 (100 MHz(100 MHz(.(.
Detection of Solanum diphyllum L. Root Compounds Using
Different Reagents on the Preparative TLC.
Mass spectra for compoundMass spectra for compound 55 using ESIusing ESI..
Structure of CompoundStructure of Compound 55..
3 – O - (β – D – glucopyranosyl) etioline [ (25S) - 22, 26 – epimino - 3β ...
Mass spectra for compoundMass spectra for compound 66 using ESIusing ESI..
Structure of CompoundStructure of Compound 66..
3 – O - (β – D – glucoronic acid) etioline [(25S)-22, 26 – epimino - 3β - ...
EcophysiologicalEcophysiological
Effect of Light on Germination percentage ofEffect of Light on Germination percentage of
Solanum diphyllumSolanum diphyllu...
Effect of Temperature on Germination Percentage ofEffect of Temperature on Germination Percentage of SolanumSolanum
diphyl...
Effect of Soil Texture Type and Planting Depth onEffect of Soil Texture Type and Planting Depth on S.S.
diphyllumdiphyllum...
The Effect of Different Concentrations of PEG 6000, and NaCl onThe Effect of Different Concentrations of PEG 6000, and NaC...
The Effect of Different Concentrations of PEG 6000,The Effect of Different Concentrations of PEG 6000,
and NaCl onand NaCl...
Analysis of CertainAnalysis of Certain
Metabolites ofMetabolites of
Solanum diphyllumSolanum diphyllum L.L.
SeedsSeeds
PART 2PART 2
Desmodium tortuosumDesmodium tortuosum
Local DistributionLocal Distribution
Different Shapes of Desmodium tortuosum Root.
T.S inT.S in Desmodium tortuosumDesmodium tortuosum RootRoot..
T.S inT.S in Desmodium tortuosumDesmodium tortuosum StemStem
T.S inT.S in Desmodium tortuosumDesmodium tortuosum PulvinusPulvinus
T.S inT.S in Desmodium tortuosumDesmodium tortuosum PetiolePetiole
DesmodiumtortuosumDesmodiumtortuosum
TrichomesTrichomes
Desmodium tortuosumDesmodium tortuosum Pollen GrainPollen Grain
Stomata onStomata on D. tortuosumD. tortuosum LeafLeaf
T.S inT.S in D. tortuosumD. tortuosum LeafLeaf..
Desmodium tortuosumDesmodium tortuosum FruitFruit..
A
B C
Desmodium tortuosumDesmodium tortuosum SeedSeed..
In vitroIn vitro Cytotoxicity TestCytotoxicity Test
Using SBR AssayUsing SBR Assay
Screening for Antimicrobial Activity
Screening for Antimicrobial Activity
Antimicrobial Activity as Mean Zone of Inhibition (mm ± SD)Antimicrobial Activity as Mean Zone of Inhibition (mm ± SD)
of ...
Antimicrobial Activity as MICs (μg/ml) of TestedAntimicrobial Activity as MICs (μg/ml) of Tested DesmodiumDesmodium
tortuo...
PhytochemicalPhytochemical
11
H-NMR Spectrum of CompoundH-NMR Spectrum of Compound 11′′..
Mass Spectrum of Compound 1Mass Spectrum of Compound 1′′..
Structure ofStructure of CompoundCompound 11.. ′′
7-methoxy- apegenin- 6-C- glucopyranosyl-O- β- (2″→1 )-‴
glucopyranoside...
Mass Spectrum of Compound 2Mass Spectrum of Compound 2′′..
Preliminary Structure ofPreliminary Structure of CompoundCompound 22.. ′′
Spirosolane-3-O-glycopyranoside
EcophysiologicalEcophysiological
Germination Percentage atGermination Percentage at SeedSeed Different Pre-Different Pre-
Treatment MethodsTreatment Method...
D. tortuosumD. tortuosum Seed StructureSeed Structure
A) Cuticle Layer and the Proposed Cracks in theA) Cuticle Layer and ...
Effect of Temperature onEffect of Temperature on Desmodium tortuosumDesmodium tortuosum
Seed GerminationSeed Germination..
Germination Percentage ofGermination Percentage of D. tortuosumD. tortuosum underunder
Different Light PeriodsDifferent Li...
SeedSeed Germination Percentages ofGermination Percentages of D. tortuosumD. tortuosum inin
Different Soil Types and Soil ...
The Effect of Different Concentrations of PEG 6000 andThe Effect of Different Concentrations of PEG 6000 and
NaCl on Seed ...
Analysis of Certain Metabolites inAnalysis of Certain Metabolites in D. tortuosumD. tortuosum
SeedSeed
PublicationsPublications
PublicationsPublications
Acknowledgements
My gratitude to all whose support has contributed to
this work.
it is your turn, guys!it is your turn, guys! NowNow,,
Thank you very much for your attention,
and hopefully you enjoyed it.
‫ال‬ً
PhD seminar presentation
PhD seminar presentation
PhD seminar presentation
PhD seminar presentation
PhD seminar presentation
PhD seminar presentation
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PhD seminar presentation

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PhD seminar presentation

  1. 1. Taxonomical, Ecophysiological andTaxonomical, Ecophysiological and Phytochemical StudiesPhytochemical Studies onon Solanum diphyllumSolanum diphyllum L.L. andand Desmodium tortuosumDesmodium tortuosum (Sw.) DC.(Sw.) DC. Presented byPresented by Fatma AbdFatma Abd--Allah Mohamed HamadaAllah Mohamed Hamada
  2. 2. Supervised bySupervised by ProfProf.. Mohamed GMohamed G.. Sheded ProfSheded Prof.. Arafa I. HamedArafa I. Hamed Prof. of Plant EcologyProf. of Plant Ecology Prof. of PhytochemistryProf. of Phytochemistry Botany Department Botany DepartmentBotany Department Botany Department Faculty ofFaculty of ScienceScience Faculty ofFaculty of ScienceScience AswanAswan University Aswan UniversityUniversity Aswan University Late DrLate Dr.. Abdel Samai MAbdel Samai M.. ShaheenShaheen Assistant ProfAssistant Prof.. ofof Taxonomy and FloraTaxonomy and Flora Botany DepartmentBotany Department Faculty ofFaculty of Science atScience at AswanAswan AswanAswan UniversityUniversity DISSERTATION PRESENTED TO THE FACULTY OF SCIENCE,DISSERTATION PRESENTED TO THE FACULTY OF SCIENCE, OF ASWANOF ASWAN UNIVERSITY FOR THE DEGREE OFUNIVERSITY FOR THE DEGREE OF DOCTOR OF PHILOSOPHYDOCTOR OF PHILOSOPHY ASWAN UNIVERSITYASWAN UNIVERSITY
  3. 3. Part 1Part 1 Solanum diphyllumSolanum diphyllum L.L.
  4. 4. Local DistributionLocal Distribution
  5. 5. Shoot Another shoot A B Vertical main root Underground lateral connection Shoot Underground lateral connection (root lateral) A)A) Solanum diphyllumSolanum diphyllum L. RootL. Root..
  6. 6. A)A) Solanum diphyllumSolanum diphyllum L. Middle-Aged Root. B) Old RootL. Middle-Aged Root. B) Old Root (Bar = 0.05 mm(Bar = 0.05 mm(.(. A B
  7. 7. B C D E F A Solanum diphyllumSolanum diphyllum L. StemL. Stem
  8. 8. Solanum diphyllumSolanum diphyllum L. TrichomesL. Trichomes A B
  9. 9. Solanum diphyllumSolanum diphyllum L. LeafL. Leaf A C D B
  10. 10. Solanum diphyllumSolanum diphyllum L. PetioleL. Petiole
  11. 11. Analysis of Different MetabolitesAnalysis of Different Metabolites
  12. 12. Solanum diphyllumSolanum diphyllum L. StomataL. Stomata A B
  13. 13. Solanum diphyllumSolanum diphyllum L.L. Pollen GrainPollen Grain
  14. 14. Solanum diphyllumSolanum diphyllum L. SeedL. Seed
  15. 15. In vitroIn vitro Cytotoxicity TestCytotoxicity Test Using SBR AssayUsing SBR Assay Root Shoot Fruit
  16. 16. PhytochemicalPhytochemical
  17. 17. 11 H-NMR Spectrum of CompoundH-NMR Spectrum of Compound 11 (600 MHz(600 MHz(.(.
  18. 18. 1313 C-NMR Spectrum of CompoundC-NMR Spectrum of Compound 11 (100 MHz(100 MHz(.(.
  19. 19. Structure of CompoundStructure of Compound 11.. (3 – O - {[α - L -rhamnopyranosyl-(1 4)]-[α - L -rhamnopyranosyl- (1 2)]- β -→ → D-glucopyranosyl} - (25R) furost -5- en- 3β, 22α, 26- triol -26- β- D- glucopyranoside).
  20. 20. 11 H-NMR Spectrum of CompoundH-NMR Spectrum of Compound 22 (600 MHz(600 MHz(.(.
  21. 21. 1313 C-NMR Spectrum of CompoundC-NMR Spectrum of Compound 22 (100 MHz(100 MHz(.(.
  22. 22. Structure of CompoundStructure of Compound 22.. (3 – O - {[α - L -rhamnopyranosyl-(1 4)]-[α - L -rhamnopyranosyl- (1 2)]- β - D-→ → glucopyranosyl} - (25R) furost -5- en- 3β, 22α, 26- triol -26- β- D- glucopyranoside).
  23. 23. Full Mass Spectra for Compound 3 and 4 Using ESIFull Mass Spectra for Compound 3 and 4 Using ESI (Electro Spray Ionization(Electro Spray Ionization(.(.
  24. 24. 11 H-NMR Spectrum of CompoundH-NMR Spectrum of Compound 33 (500 MHz(500 MHz(.(.
  25. 25. 1313 C-NMR Spectrum of CompoundC-NMR Spectrum of Compound 33 (100 MHz(100 MHz(.(.
  26. 26. Detection of Solanum diphyllum L. Root Compounds Using Different Reagents on the Preparative TLC.
  27. 27. Mass spectra for compoundMass spectra for compound 55 using ESIusing ESI..
  28. 28. Structure of CompoundStructure of Compound 55.. 3 – O - (β – D – glucopyranosyl) etioline [ (25S) - 22, 26 – epimino - 3β - ( β – D -glucopyrranosyloxy) cholesta – 5 , 22 (N) – dien – 16 α - ol)] (C33H53O7N).
  29. 29. Mass spectra for compoundMass spectra for compound 66 using ESIusing ESI..
  30. 30. Structure of CompoundStructure of Compound 66.. 3 – O - (β – D – glucoronic acid) etioline [(25S)-22, 26 – epimino - 3β - (β-D- glucopyrranosyloxy) cholesta-5, 22 (N)-dien-16α-ol)] (C33H53O8N )
  31. 31. EcophysiologicalEcophysiological
  32. 32. Effect of Light on Germination percentage ofEffect of Light on Germination percentage of Solanum diphyllumSolanum diphyllum L. under Different Light PeriodsL. under Different Light Periods
  33. 33. Effect of Temperature on Germination Percentage ofEffect of Temperature on Germination Percentage of SolanumSolanum diphyllumdiphyllum L. Seeds at the End of the Experiment PeriodL. Seeds at the End of the Experiment Period
  34. 34. Effect of Soil Texture Type and Planting Depth onEffect of Soil Texture Type and Planting Depth on S.S. diphyllumdiphyllum L. Seed GerminationL. Seed Germination..
  35. 35. The Effect of Different Concentrations of PEG 6000, and NaCl onThe Effect of Different Concentrations of PEG 6000, and NaCl on Seed Germination ofSeed Germination of Solanum diphyllumSolanum diphyllum LL..
  36. 36. The Effect of Different Concentrations of PEG 6000,The Effect of Different Concentrations of PEG 6000, and NaCl onand NaCl on Root/Shoot Ratio ofRoot/Shoot Ratio of S. diphyllumS. diphyllum LL..
  37. 37. Analysis of CertainAnalysis of Certain Metabolites ofMetabolites of Solanum diphyllumSolanum diphyllum L.L. SeedsSeeds
  38. 38. PART 2PART 2 Desmodium tortuosumDesmodium tortuosum
  39. 39. Local DistributionLocal Distribution
  40. 40. Different Shapes of Desmodium tortuosum Root.
  41. 41. T.S inT.S in Desmodium tortuosumDesmodium tortuosum RootRoot..
  42. 42. T.S inT.S in Desmodium tortuosumDesmodium tortuosum StemStem
  43. 43. T.S inT.S in Desmodium tortuosumDesmodium tortuosum PulvinusPulvinus
  44. 44. T.S inT.S in Desmodium tortuosumDesmodium tortuosum PetiolePetiole
  45. 45. DesmodiumtortuosumDesmodiumtortuosum TrichomesTrichomes
  46. 46. Desmodium tortuosumDesmodium tortuosum Pollen GrainPollen Grain
  47. 47. Stomata onStomata on D. tortuosumD. tortuosum LeafLeaf
  48. 48. T.S inT.S in D. tortuosumD. tortuosum LeafLeaf..
  49. 49. Desmodium tortuosumDesmodium tortuosum FruitFruit.. A B C
  50. 50. Desmodium tortuosumDesmodium tortuosum SeedSeed..
  51. 51. In vitroIn vitro Cytotoxicity TestCytotoxicity Test Using SBR AssayUsing SBR Assay
  52. 52. Screening for Antimicrobial Activity Screening for Antimicrobial Activity
  53. 53. Antimicrobial Activity as Mean Zone of Inhibition (mm ± SD)Antimicrobial Activity as Mean Zone of Inhibition (mm ± SD) of the Testedof the Tested Desmodium tortuosumDesmodium tortuosum Extract Samples AgainstExtract Samples Against the Tested Microorganismsthe Tested Microorganisms..
  54. 54. Antimicrobial Activity as MICs (μg/ml) of TestedAntimicrobial Activity as MICs (μg/ml) of Tested DesmodiumDesmodium tortuosumtortuosum Extract Samples Against Tested MicroorganismsExtract Samples Against Tested Microorganisms..
  55. 55. PhytochemicalPhytochemical
  56. 56. 11 H-NMR Spectrum of CompoundH-NMR Spectrum of Compound 11′′..
  57. 57. Mass Spectrum of Compound 1Mass Spectrum of Compound 1′′..
  58. 58. Structure ofStructure of CompoundCompound 11.. ′′ 7-methoxy- apegenin- 6-C- glucopyranosyl-O- β- (2″→1 )-‴ glucopyranoside (Spinosin).
  59. 59. Mass Spectrum of Compound 2Mass Spectrum of Compound 2′′..
  60. 60. Preliminary Structure ofPreliminary Structure of CompoundCompound 22.. ′′ Spirosolane-3-O-glycopyranoside
  61. 61. EcophysiologicalEcophysiological
  62. 62. Germination Percentage atGermination Percentage at SeedSeed Different Pre-Different Pre- Treatment MethodsTreatment Methods ( Release of Dormancy).( Release of Dormancy).
  63. 63. D. tortuosumD. tortuosum Seed StructureSeed Structure A) Cuticle Layer and the Proposed Cracks in theA) Cuticle Layer and the Proposed Cracks in the D. tortuosumD. tortuosum SeedSeed Epidermis.Epidermis. B) Hilum, Micopyle and Strophiolar Fissure ofB) Hilum, Micopyle and Strophiolar Fissure of D. tortuosumD. tortuosum Seed.Seed.
  64. 64. Effect of Temperature onEffect of Temperature on Desmodium tortuosumDesmodium tortuosum Seed GerminationSeed Germination..
  65. 65. Germination Percentage ofGermination Percentage of D. tortuosumD. tortuosum underunder Different Light PeriodsDifferent Light Periods..
  66. 66. SeedSeed Germination Percentages ofGermination Percentages of D. tortuosumD. tortuosum inin Different Soil Types and Soil DepthsDifferent Soil Types and Soil Depths
  67. 67. The Effect of Different Concentrations of PEG 6000 andThe Effect of Different Concentrations of PEG 6000 and NaCl on Seed Germination ofNaCl on Seed Germination of D. tortuosumD. tortuosum..
  68. 68. Analysis of Certain Metabolites inAnalysis of Certain Metabolites in D. tortuosumD. tortuosum SeedSeed
  69. 69. PublicationsPublications
  70. 70. PublicationsPublications
  71. 71. Acknowledgements My gratitude to all whose support has contributed to this work.
  72. 72. it is your turn, guys!it is your turn, guys! NowNow,,
  73. 73. Thank you very much for your attention, and hopefully you enjoyed it. ‫ال‬ً

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