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Nmr spectroscopy

  1. 1. 1 NMRNMR SPECTROSCOPYSPECTROSCOPY Presented by,Presented by, FARSANA PFARSANA P DEPT OF PHARMACEUTICSDEPT OF PHARMACEUTICS GRACE COLLEGE OF PHARMACYGRACE COLLEGE OF PHARMACY
  2. 2. 2 CONTENTSCONTENTS • Principle of NMR • FT-NMR
  3. 3. 3 NMR spectroscopy is the study of spin changes at the nuclear level when a radiofrequency energy is absorbed in the presence of magnetic field. The energy required for NMR lies in the low energy or long wavelength radio frequency.
  4. 4. 4 PrinciplePrinciple Magnetic field is applied Any proton or nucleus with odd mass number Its spin on its own axis and magnetic moment is created with a frequency called as precessional frequency
  5. 5. 5 This state is called ground state or parallel orientation In this state magnetic field caused by spin of nuclei is aligned with externally applied magnetic field. Radiofrequency is applied (energy) Absorption of energy, nucleus move from G.S to E.S
  6. 6. 6 Results- spin reversal anti-parallel orientation Application of radiofrequency is stopped Nucleus returns to G.S or parallel orientation
  7. 7. 7
  8. 8. 8 FT-NMRFT-NMR
  9. 9. 9  Mathematical operation  Complex waveform convert to simple mathematical operations Time domainTime domain spectrumspectrum FrequencyFrequency domain spectrumdomain spectrum
  10. 10. 10
  11. 11. 11 Theory of FT-NMRTheory of FT-NMR Magnetic nuclei in magnetic field Pulse of radio frequency is irradiated Nuclei absorb some of the energy Precess (spin) like little tops
  12. 12. 12 (signal) Free induction decay (FID) is converted to spectrum A complex signal is produced, then decay as nuclei lose energy
  13. 13. 13 ADVANTAGES:-ADVANTAGES:- • FT-NMR is more sensitive and can measure more weaker signals. • Dramatic increase in the sensitivity of NMR measurements. • Can be obtained with less than 5mg of compound. • The signal stand out clearly with almost no electronic background noise • Used in engineering, industrial quality control and medicine.
  14. 14. 14 • The FT-NMR method also gives improved spectra for sparingly soluble compounds.
  15. 15. 15 Reference:-Reference:- • Text book of pharmaceutical analysis by Ravisankar, page no: 6-2 to 6-4 • https://www.solairajananant/nmr-spectroscopy • https://www.bijayauprety-nmr 41214142 • https://www.pci.tu.bs.de/aggericke/PC4/kap-i/ftnm
  16. 16. 16 Thank youThank you

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