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Jauhar ali. vol 5. screening for abiotic and biotic stress tolerances

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Jauhar ali. vol 5. screening for abiotic and biotic stress tolerances

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Jauhar ali. vol 5. screening for abiotic and biotic stress tolerances

  1. 1. Basic Molecular Breeding 2:Basic Molecular Breeding 2:Basic Molecular Breeding 2:Basic Molecular Breeding 2: S3-Y2 IRRIIRRIIRRIIRRIIRRIIRRIIRRIIRRI Jauhar Ali Plant Breeder, Senior Scientist IRRI-GSR Project Leader & Regional Coordinator (Asia) PBGB, IRRI (J.Ali@irri.org) GSRIR1-8-S6-S3
  2. 2. Why there is a necessity for breeding for resilience? • Food Security 2008–looming threat-higher yields under ever reducing resources • Stable sustainable yields using lesser inputs(SSNM)- farmer practice-rainfed complex abiotic stresses-stressed irrigated condition- Lesser inputs-complex conditions • Diseases & Insect pest threats-high input environments-• Diseases & Insect pest threats-high input environments- Broad spectrum-durable R • Caring for environment-pollution of water systems- chemical residues-Reduction of C’s • Changing climatic situations-vulnerability-heat-flooding- drought- Multiple Stress Tolerance
  3. 3. RACE AGAINST TIME
  4. 4. Drought stress frequency map in Fess et al (2011) Crop Breeding for Low Input Agriculture: A Sustainable Response to Feed a Growing World Population. Sustainability 2011, 3(10), 1742-1772 Drought stress frequency map in rainfed rice areas based on rainfall anomalies from 1960-2002. Flood frequency map in rice growing areas based on 100 years of UNEP flood risk data.Andy et al (2012) IRRI GSR Report
  5. 5. Rice Ecosystems Breeding for Drought prone environments: Fischer et al 2003
  6. 6. TargetTraits Yield & Grain Abiotic Drought Salinity Submergence Anaerobic germination Low input environments Early maturity can be useful trait to escape both the stresses. Grain quality matters a lot at the end Most donors will be able to throw transgressive TargetTraits Yield & Grain Quality Biotic BLB BLAST TUNGRO/RSV BLS Sheath blight transgressive segregants Identify extreme transgressive segregants & their donors Place the tolerant checks, DP, RP for convenient selection
  7. 7. ABIOTIC STRESSES • Drought • Submergence • Salinity-alkalinity • Anaerobic Germination• Anaerobic Germination • Low soil fertility soils-N, P, K • Problem soils-Al, Fe toxicity, acid sulphate, peat soils
  8. 8. Salt Stresses and Associated Complexities S A L T S Acid SO4 S A L I N E (P, Zn) (P, Zn) Fe, Al tox Fe, H2S tox Al, Organic R A I N F E D Sub- merged Deep- water Drought G r a I n S T R E S S E S Peat E ALKALINE INLAND SALINE (P, Zn) (P, Zn) (Fe) Al, Organic Acids tox (P & Zn) D Drought Irrigated Q u a l i t y (Source: Glenn B. Gregorio)
  9. 9. Breeding for Resilience-“GSR” Rice c ultivars that produce higher & more stable yields with lesser inputs (water, fertilizers &pesticides) High yielding GSR cultivars with “Green” traits: Resistances/ tolerances to:Resistances/ tolerances to: Abiotic stresses: Drought, salinity, alkalinity, iron toxicity, etc. Diseases: Blast, BLB, sheath blight, BLS viruses & false smut Insects: BPH, GLH, stem borer etc Grain quality Mostly in elite RP background- later in RP-NARES High resource-use efficiency: Water and nutrients (N P K) Less inputs, more production & environment sustainabilityLess inputs, more production & environment sustainability
  10. 10. RP (3) x donors(205) F1s x RP BC1F1s x RP ~25 BC2F1s/donor x RP x Bulk BC2F2 populations BC3F1s x RP 1, 2, 3, 4, 5, 6, …… BC3F2 populations Self and bulk harvest Selection for target traits and backcrossing BC4F1s BC4F2s x x Self and bulk harvest 1, 2, 3, 4, 5, 6, …… Development of GSR materials by designed QTL pyramiding (DQP) strategy for select target component traits for a given ecosystem Alietal(2006)FCR97:66-76 Confirmation of the selected traits by replicated phenotyping and genotyping of ILs for gene/QTL identification Crosses made between sister ILs having unlinked desirable genes/ QTLs for target ecosystem DQP &MAS for pyramiding desirable genes/QTLs and against undesirable donor segments for target ecosystem Development of GSR materials with improved target traits for wide scale testing in different ecosystems and its release. NILs for individual genes/QTLs for functional genomic studies Screening for target traits such as tolerances to drought, salinity, submergence, anaerobic germ., P & Zn def., BPH, etc. Alietal(2006)FCR97:66
  11. 11. Development of ILS for different abiotic and biotic stress tolerances at IRRI Ali et al (2006) FCR 97:66-76
  12. 12. Hidden diversity for abiotic and biotic tolerance inHidden diversity for abiotic and biotic tolerance in the primary gene pool of ricethe primary gene pool of rice • Tremendous amounts of hidden diversity-BC progeny-transgressive -target traits- regardless of donor performance-severe stress screening • Common to identify in BC progeny-extreme phenotypes (tolerances) • Selection efficiency –highly dependent upon background • Selection efficiency-affected by level of stress applied • Selection efficiency for different target traits vary in BC generations. • More distantly related donors, particularly landraces, tend to give more transgressive segregations for complex phenotypes in the BC progenies. • Wide presence and random distribution of stress tolerance genes in primary gene pool of rice –good news for rice breeders Yu et al (2003) TAG 108:131-140; Ali et al (2006) FCR 97:66-76
  13. 13. RM380.0 RM2516.1 RM7234.8 RM4440.4 RM33943.8 8 RM2850.0 RM21911.8 RM10534.7 9 RM20A0.0 RM1919.1 RM24736.0 12 Chromosomal location of QTLs for BPH in IR64/Binam BC2F4 population (4.15) (11.64) (13.5) RM4259.1 RM25668.7 RM25786.8 RM24294.2 RM20196.7 RM205111.1 RM26073.7 RM17123.9 QBph (4.15) (11.64) (7.43) IL 64 (IR64 x Binam) IL 80 (IR64 x Binam) BPHscreening BPH 36.7%
  14. 14. Rice hybrids for rainfed lowlands: AnaerobicRice hybrids for rainfed lowlands: Anaerobic germination a key traitgermination a key trait • 19% area; 1-1.5t/ha • Poor seedling establishment • 11 ILs(BC4F2) with complete germination 4.90 28.7 33.7 37.0 RM60 RM231 RM7 RM232 RM251 0.0 QAna1(10.0) Chr3 IR64/Binam BC2F4 popln. • 11 ILs(BC4F2) with complete germination in NPT background • Two of them were promising 7235, 7237(TKM-9) • Efforts are underway to pyramid it with submergence tolerance. RM130.0 RM2899.0 RM16337.7 RM16442.0 RM16148.1 RM17862.1 (11.0) (27.8) QAna2 QAna3 Chr5 54.3 72.8 85.10 RM130124.4 160.0 RM282 RM16 RM203 RM85
  15. 15. FAVOURABLE DONORS (VARY ACCORDING TO RP)FAVOURABLE DONORS (VARY ACCORDING TO RP)S.No.S.No. OM1706,OM1723,OM1706,OM1723,FR13AFR13A,NAN29,NAN29--2,BABOAMI, KHAZAR2,BABOAMI, KHAZARSTST TKM9,TKM9,HEIHEI--HEHE--AIAI--HUI(HHAH),JIANGXIHUI(HHAH),JIANGXI--SISI--MIAO(JSM), KHAZAR, MADHUKAR,MIAO(JSM), KHAZAR, MADHUKAR, SHWESHWE--THWETHWE--YINYIN--HYE (STYH), BASMATI385, IKSAN438, YUHYE (STYH), BASMATI385, IKSAN438, YU--QIUQIU--GU, TETEP,GU, TETEP, NIPPONBARE, CO43, RASI, YUNHUI, BG304,BR24,NIPPONBARE, CO43, RASI, YUNHUI, BG304,BR24, FR13AFR13A GAYABYEOGAYABYEO ZDTZDT Y134,TKM9,KHAZAR,GAYABYEO,STYH,NAN29Y134,TKM9,KHAZAR,GAYABYEO,STYH,NAN29--2,2, AGAG Donors that gave better results with varying recurrent parental backgrounds Y134,TKM9,KHAZAR,GAYABYEO,STYH,NAN29Y134,TKM9,KHAZAR,GAYABYEO,STYH,NAN29--2,2, BABOAMI,JSM,BABOAMI,JSM,FR13AFR13A,OM1706,OM1706AGAG CISEDANE,CISEDANE,FR13AFR13A,IR50,NAN29,IR50,NAN29--2,OM1706,STYH,TAROM MOLAEI,2,OM1706,STYH,TAROM MOLAEI,TKM9TKM9,Y134,Y134SUBTSUBT NAN29NAN29--2,GAYABYEO2,GAYABYEOLTGLTG JSM,BABOAMI,JSM,BABOAMI,TKM9TKM9,BG300,C418,LEMONT,MADHUKAR,MR167,OM1706,STYH,,BG300,C418,LEMONT,MADHUKAR,MR167,OM1706,STYH, Y134Y134BPHBPH BABOAMI, GAYABYEO, SHWEBABOAMI, GAYABYEO, SHWE--THWETHWE--YINYIN--HYE (STYH), NAN29HYE (STYH), NAN29--2, FR13A,2, FR13A, OM1706, KHAZAR, JIANGXIOM1706, KHAZAR, JIANGXI--SISI--MIAOMIAO MULTIMULTI-- TRAITSTRAITS Ali et al (2006) FCR 97:66-76
  16. 16. ExperimentsetI IR64 x BR24 F1 x IR64 BC2F2 IR64 x Binam IR64 x STYH F1 x IR64 BC2F2 IR64 x OM1723 F1 x IR64 BC2F2 F1 x IR64 BC2F2 13 BC2F2 populations screened under two types of severe drought, resulting in 221 survived DT BC2F3 introgression lines (ILs), which were genotyped with SSR markers IR64 x Type3 F1 x IR64 BC2F2 IR64 x HAN F1 x IR64 BC2F2 IR64 x Zihui100 F1 x IR64 BC2F2 ExperimentsetII IL1 x IL2 F1 X IL3 x IL4 F1 X IL7 x IL15 F1 X 9 1st round pyramiding F2 populations from crosses between 15 ILs Designed QTL pyramiding experimentsExperimentsetII Screened under severe drought at the reproductive stage, resulting in 455 survived DT F2 plants, which were progeny tested and genotyped with SSR markers F2 X F2 X F2 X crosses between 15 ILs ExperimentsetIII Screened under severe drought at the reproductive stage and 667 survived DT F3 lines were progeny tested and genotyped with SSR markers (PL1 , PL2, PL3) x (PL4, PL5, PL6, PL7, PL8) F1s F2s X 14 2nd round pyramiding F2 populations from crosses between 8 1st round PLs
  17. 17. IL1 F2 IL2 The 1st pyramiding F2 populations and their parents screened under severe lowland drought at the reproductive stage (2002-03 DS)
  18. 18. F2 population Field screening of the 2nd round pyramiding F2 populations under severe lowland stress (2005-2006 dry season) Parental 1st round PLs and IR64 Parental lines
  19. 19. Putative genetic networks identified in 455 DT PLs derived from 9 crosses between DT IR64 ILs Drought AG2-1 (5) 0.994 RM575 (1.4) 0.745 RM342 (8.5) 0.673 AG2-2 (6) 0.891 RM347 (3.8) 0.691 RM469 (6.1) 0.818 RM215 (9.8) 0.527 RM561 (2.6) 0.618 RM544 (8.2) 0.727 RM309 (12.5) 0.927 RM202 (11.3) 0.745 RM463 (12.5) 0.745 RM179 (12.3) 0.727 B:Drought AG1-1 (7) 1.00 AG1-3 (13) 0.748 AG1-2 (7) 0.979 AG1-5 (5) 0.726 RM418 (7.3) 0.717 AG1-4 (4) 0.688 RM109 (2.1) 0.617 RM179 (12.3) 0.607 A: DroughtD: AG AG7-1 (18) 1.00 Drought G: I: Drought AL9-1 (3) 1.000 RM152 (8.1) 0.930 AG9-5(3) 0.553 AG9-2(2) 0.915 RM211 (2.2) 0.800 RM446 (1.6) 0.830 RM350 (8.4) 0.800 AG9-4 (5) 0.500 AG9-3(24) 0.870 RM215 (9.7) 0.870 RM554 (3.7) 0.700 Drought RM543 (1.1) E: Drought AG3-1 (4) 1.00 AG3-2 (4) 0.855 RM302 (1.10) 0.782 AG3-3 (3) 0.736 RM172 (7.7) 0.727 C: AG4-1 (6) RM271 (10.4) RM23 (1.5) AG4-2 (4) AG4-3 (4) AG4-4 (3) RM544 (8.3) RM179 (12.3) RM215 (9.8) RM220 (1.2) RM272 (1.3) RM441 (11.2) RM36 (3.3) AG7-5 (2) AG7-2 (2) AG7-3 (16) AG7-7 (2) AG7-4 (7) RM275 (6.6) RM110 (2.1) RM224 (11.7) RM294B (1.6) RM435 (6.1) RM13 (5.2) RM5 (1.7) RM245 (9.8) RM30 (6.8) RM18 (7.6) RM465A (2.5) RM469 (6.1) RM286 (11.1) RM289 (5.3) RM44 (8.3) RM516 (5.3) RM85 (3.12) AG8-1 (26) 1.00 RM448 (3.10) RM331 (8.4) RM481 (7.1) RM535 (2.12) DroughtH: RM32 (8.3) AG8-2 (2) RM30 (6.7) AG8-3 (3) RM562 (1.6) RM547 (8.3) RM275 (6.5) RM143 (3.12) RG8-6 (2) RM197 (6.1) RM5 (1.7) RM307 (2.1) RM449 (1.6) RM14 (1.13) RM169 (5.3) AG8-4 (3) RM246 (1.8) AG8-5 (2) RM589 (6.1) RM317 (4.6) RM258 (10.4) RM154 (2.1) RM245 (9.8) RM335 (3.12) RM446 (1.6) RM211 (2.2) Drought AG6-1 (8) 1.000 AG6-3 (12) 0.894 AG6-2 (5) 0.967 RM44 (8.3) 0.633 RM235 (12.6) 0.667 AG6-4 (2) 0.772 RM51 (7.1) 0.833 RM20 12.1 0.567 F: (1.1) 1.000 AG5-4 (2) 0.767 RM53 (2.3) 0.833 AG5-1 (12) 0.711 AG5-2 (9) 0.809 RM401 (4.1) 0.733 RM433 (8.7) 0.867 RM298 (7.1) 0.767 RM17 (12.7) 0.500 RM270 (12.6) 0.567 RM222 (10.1) 0.567 RM424 (2.5) 0.667 AG5-3(2) 0.525 RM244 (10.1) 0.583 RM248 (7.7) 0.500 RM101 (12.4) 0.766 Li et al 2011 unpubl.
  20. 20. Ch.2Ch.1 Ch.3 Ch.4 Ch.5 Ch.6 RM109 RM485 RM154 RM211 RM236 RM279 RM423 RM8 RM53 RM233A RM174 RM145 RM71 RM327 RM521 RM300 RM324 RM424 RM262 RM341 RM475 RM106 RM263 RM526 RM221 RM525 RM318 RM450 RM497 RM6 RM240 RM530 RM112 RM250 RM166 RM197 RM213 RM48 RM207 RM266 RM138 RM307 RM401 RM537 RM335 RM518 RM261 RM471 RM142 RM273 RM252 RM241 RM470 RM303 RM317 RM348 RM349 RM131 RM280 RM567 RM559 RM122 RM153 RM413 RM13 RM267 RM437 RM289 RM509 RM598 RM163 RM164 RM291 RM161 RM188 RM421 RM178 RM26 RM274 RM87 RM480 RM538 RM334 RM399 RM169 RM204 RM587 RM588 RM589 RM510 RM204 RM585 RM111 RM225 RM314 RM253 RM50 RM549 RM539 RM136 RM527 RM3 RM343 RM528 RM30 RM340 RM400 RM439 RM103 RM141 RM176 RM494 RM557 RM584 RM60 RM81B RM22 RM523 RM569 RM231 RM175 RM545 RM517 OSR13 RM7 RM232 RM251 RM282 RM338 RM156 RM411 RM487 RM16 RM504 RM203 RM186 RM55 RM168 RM416 RM520 RM293 RM114 RM130 RM565 RM14 OSR23 RM431 RM472 RM297 RM265 RM319 RM315 RM128 RM212 RM403 RM473A RM246 RM237 RM306 RM5 RM9 RM594 RM323 RM84 RM428 RM220 RM86 RM522 RM283 RM1 RM272 RM575 RM490 RM576 RM259 RM583 RM243 RM600 RM572 RM580 RM581 RM23 RM129 RM446 RM329 RM562 Bin1.1 Bin1.2 Bin1.3 Bin1.4 Bin1.5 Bin1.6 Bin1.7 Bin1.8 Bin1.9 Bin1.10 Bin1.11 Bin1.12 Bin1.13 Bin2.1 Bin2.2 Bin2.3 Bin2.4 Bin2.5 Bin2.6 Bin2.7 Bin2.8 Bin2.9 Bin2.10 Bin2.11 Bin2.12 Bin3.1 Bin3.2 Bin3.3 Bin3.4 Bin3.5 Bin3.6 Bin3.7 Bin3.8 Bin3.9 Bin3.10 Bin3.11 Bin4.1 Bin4.2 Bin4.3 Bin4.4 Bin4.5 Bin4.6 Bin4.7 Bin4.8 Bin6.1 Bin6.2 Bin6.3 Bin6.4 Bin6.5 Bin6.6 Bin6.7 Bin6.8 Bin6.9 Bin5.1 Bin5.2 Bin5.3 Bin5.4 Bin5.5 Bin5.6 Bin5.7 RM245 Binam segments BR24 segments STYH segments OM1723 segments RM462 RM555 RM516 RM190 RM454 RM162 RM561 RM540 RM469 RM302 RM488 RM347 RM535 RM233B RM19029 RM19778 RM499 RM10287 RM14963 RM11570 RM551 Cross III-2 Cross III-1 FGUs identified in cross II-1 FGUs identified in cross II-2 FGUs identified in cross II-3 RM565 RM514 RM570 RM227 RM85 Ch.8 Ch.9 Ch.10 Ch.11 Ch.12Ch.7 RM474 RM222 RM216 RM239 RM311 RM467 RM184 RM271 RM269 RM258 RM171 RM304 RM228 RM147 RM333 RM496 RM436 RM51 RM481 RM125 RM180 RM501 OSR22 RM214 RM418 RM432 RM11 RM346 RM182 RM336 RM10 RM351 RM455 RM505 RM234 RM18 RM172 RM248 RM408 RM506 RM407 OSR30 RM544 RM25 RM407 RM44 RM72 RM137 RM331 RM339 RM342A RM515 RM284 RM210 RM556 RM256 RM149 RM230 RM264 RM281 RM296 RM285 RM316 RM444 RM219 RM524 RM105 RM321 RM409 RM460 RM566 RM434 RM257 RM108 RM242 RM278 RM201 RM107 OSR28 RM189 RM215 RM205 RM286 RM332 RM167 RM120 RM479 RM181 RM202 RM536 RM260 RM287 RM209 RM229 RM457 RM187 RM21 RM206 RM254 RM224 RM144 RM20A RM4A RM19 RM247 RM512 RM179 RM101 RM277 RM511 RM519 RM313 RM309 RM463 RM235 RM270 RM17 RM4B Bin3.12 Bin10.7 Bin10.1 Bin10.2 Bin10.3 Bin10.4 Bin10.5 Bin10.6 Bin7.1 Bin7.3 Bin7.5 Bin7.7 Bin7.2 Bin7.4 Bin7.6 Bin8.8 Bin8.1 Bin8.2 Bin8.3 Bin8.4 Bin8.5 Bin8.6 Bin8.7 Bin9.8 Bin9.1 Bin9.2 Bin9.3 Bin9.4 Bin9.5 Bin9.6 Bin9.7 Bin11.7 Bin11.1 Bin11.3 Bin11.4 Bin11.5 Bin11.6 Bin12.5 Bin12.1 Bin12.2 Bin12.3 Bin12.4 Bin12.6 Bin12.7 RM547 RM447 Bin11.2 Binam segments Genomic correspondences between FGUs identified in 150 ILs of 8 BC2 populations, 200 PLs of 3 1st round pyramiding crosses and 4 2nd round pyramiding crosses. RM223 RM126 RM25022 RM23818 RM245 RM25181 RM473E RM26063 Cross III-2 Cross III-1 Cross III-3 Cross III-4 Li et al 2011 (unpubl)
  21. 21. Meanyieldunderthe irrigatedcontrol(t/ha) 1.5 2.0 2.5 3.0 Type III (N=19) C: 5.06±±±±0.47 VS: 1.98±±±±0.47 RS: 1.94±±±±0.52 Type I (N=17) C: 5.76±±±±0.53 VS: 2.07±±±±0.55 RS: 1.79±±±±0.47 Type II (N=5) C: 5.71±0.42 VS: 1.36±0.38 RS: 2.20±0.45 Type IV (N=7) C: 4.66±0.48 VS: 1.34±0.41 RS: 1.86±0.51 IR64 (CK) C: 4.68±0.23 VS: 1.49±0.14 RS: 0.52±0.38 4.0 4.5 5.0 5.5 6.0 6.5 The mean yield performances (t/ha) of 48 2nd round PLs (4 types) as compared to IR64 (CK), under the irrigated control (C), drought stresses at the vegetative (VS) and reproductive stages (RS) in the 2007 and 2008 dry-season. Guan et al. 2010 JXB 3.0 0.5 1.0 1.5 2.0 2.5 0.5 1.0 1.5 3.5 3.0 0.0
  22. 22. 6 sqm plot 100 hills yield from the center of the plot 3 replication 1 month stressed irrigation No irrigation till harvest in DS2010 IRRI
  23. 23. GSR Drought tolerant pyramided lines in IR64 background Under zero input conditions at IRRI DS2010 DT Check variety IR74371-70-1-1 GSR-IR83142-B-19-B
  24. 24. ILOILO GSR SALINITY TRIALS 2008:Salinity pyramided lines at ILOILO Replicated yield trials of stable 10 salinity tolerant pyramiding lines under Iloilo hot spot (18.8dSm-1 ; pH 6.3) for salinity conditions showing immense potential. Evaluation of 62 GSR materials in saline field condition in Ajuy, Iloilo 2reps Highly saline (EC16.2dS/m at transplanting, EC18 for 3 weeks and EC 20 after 4 weeks of transplanting) Trial conducted by Dr Glenn, PBGB
  25. 25. GSR entryGSR entry No ofNo of paniclespanicles PlantPlant heightheight (cm)(cm) MaturityMaturity (days)(days) YieldYield (kg/ha)(kg/ha) %% increaseincrease overover FL478FL478 SESSES scorescore 4WAT4WAT SESSES scorescore MaturityMaturity IR83140IR83140--BB--1111--BB 1616 8484 116116 11401140 103.6103.6 44 55 Promising GSR Drought + Salinity tolerant materials tested under Iloilo during WS2010 1616 8484 116116 11401140 103.6103.6 44 55 IR83140IR83140--BB--2828--BB 1313 8686 114114 876876 56.456.4 44 55 IR83140IR83140--BB--3232--BB 1515 8585 114114 657657 17.317.3 44 55 FL478FL478 1111 7070 111111 560560 0.00.0 55 -- NSIC 222NSIC 222 1919 8383 112112 147147 --73.873.8 44 -- First two nominated for NCT Philippines WS2011
  26. 26. DT PDLs AMMI-Biplot: 6 Locations -2011DS BRAC-Gaz, VAAS-Gia, VAAS-Duo, ICRR-Jak, ICRR-Teg, & IRRI-Los Banos Entry No. GSR Lines Mean (t/ha) LSD Group 15 IR 83142-B-57-B 5.46 a 9 IR 83141-B-17-B 5.17 b 19 IR 83142-B-7-B-B 5.13 bc 18 IR 83142-B-79-B 5.12 bc 11 IR 83142-B-19-B 5.06 bcd 5 IR 83140-B-11-B 5.05 bcde 10 IR 83141-B-18-B 5.02 bcdef 0.00.5 PC2 1 2 4 68 10 11 12 13 16 17 1819 10amBrGa 10dsIcJa 10dsIRig 10suVaDu10suVaGi 1 2 PC % 60.9 24.7 IR 83142-B-19-B 2nd Best Check methylationJXBDOI:10.1093/jxb/erq391 17% adv. 6 IR 83140-B-28-B 4.94 bcdefg 13 IR 83142-B-21-B 4.86 cdefg 12 IR 83142-B-20-B 4.79 defg 14 IR 83142-B-49-B 4.78 efg 16 IR 83142-B-60-B 4.75 fg 20 IR 83142-B-8-B-B 4.74 g 7 IR 83140-B-32-B 4.74 g 3 Best Check 4.67 g 8 IR 83140-B-36-B 4.32 h 1 2nd Best Check 4.29 h 17 IR 83142-B-61-B 4.27 h 4 IR 74371-70-1-1 3.57 i 2 Apo 3.53 i -0.5 0.0 0.5 1.0 -1.0-0.5 PC 1 PC2 3 5 7 9 11 14 15 20 10dsIcTe 10suVaDuIR 83142-B-19-B Best Check IR 83140-B-11-B Environments Mean (t/ha) LSD Group IRRI-Los Banos 6.55 a VAAS-Gia 6.53 a VAAS-Duo 6.06 b BRAC-Gaz 4.29 c ICRR-Jak 3.18 d ICRR-Teg 2.08 e IR 83142-B-57-B Why such yield advantages? IL breeding + Designed QTL Pyramiding Selection for grain yield, higher spikelet fertility, deeper and thicker roots esp. under reproductive stage DT stress Wangetal2010DroughtinducedsitespecificDNAmethylation
  27. 27. IR83140-B-11-B PVS Purvakarta 2.5ha trial area Indonesia 8.2011 High protein 11%, AC 21%, GT-I, suitable for direct wet and dry, transplanting, drought, salinity, cold germination BLB, Blast tolerant Grainyieldt/ha Site specific nutrient management (SSNM) Untungetal(2012)unpubl.
  28. 28. Name CoteD’ivoir Mali Rwanda Nigeria Mozambique Tanzania Uganda Bangladesh Indonesia LaoPDR Pakistan SriLanka Vietnam Philippines All HuangHuaZhan 2 1 3 1 3 1 2 1 1 15 Zhongzu14 2 1 1 1 1 1 7 ZH1 2 1 2 1 1 1 1 9 2 1 1 1 1 List of the promising widely adaptable GSR inbreds identified from adaptation yield trials in SSA, SEA and SA KCD1 2 1 1 1 1 1 7 RC8 1 2 1 1 1 6 Weed Tolerant 1 1 2 1 2 1 7 HUA-565 2 2 1 5 FFZ 1 1 1 1 1 5 SAGC-4 2 2 1 1 1 7 WX763 2 1 1 1 5 HHZ developed in GAAS is a mega-variety of high yield & superior quality grown in 8 provinces of South & Central China (Guangdong, Jiangxi, Fujian, Hunan, Hubei, Anhui, Yunan and Guangxi).
  29. 29. The complex pedigree of Huang-Hua-Zhan (HHZ) involving 14 parents
  30. 30. S.C.Zhouetal.,unpublishedZ.K.Lietal2012(unpubl.) Plant type, Panicle and Grain shape & size of HHZ & its immediate parents P1(FHZ) P2(HXZ) HHZ P1(FHZ) P2(HXZ) HHZ S.C.Zhouetal.,unpublishedZ.K.Lietal2012(
  31. 31. Ch. 1 Ch. 2 Ch. 3 Ch. 4 Ch. 5 Ch. 6 Ch. 7 Ch. 8 Ch. 9 Genomic composition of the HHZ genome based on the re-sequencing data (From S. C. Zhou et al., unpublished) Ch. 9 Ch. 10 Ch.11 Chr. 12 Each colored vertical line corresponds to a window of 10 kb. Vertical lines distribute upper side on each chromosome represent AZ haplotype blocks (red for ≥200kb AZ blocks, light red for <200kb AZ blocks) and QZ haplotype blocks (blue for ≥200kb QZ blocks and light blue for <200kb QZ blocks). Vertical lines distribute lower side on each chromosome represent “Stress” related QTL region (light yellow), “Quality” related QTL region (light green) and “Yield” related QTL region (light purple). Blue and red arrows indicate QZ blocks overlapped with “Yield” related QTL regions and AZ blocks overlapped with “Quality” related QTL regions, respectively.
  32. 32. IRRI-GSR breeding program & strategy IRRIIRRIIRRIIRRIIRRIIRRIIRRIIRRI
  33. 33. GSR Introgression Breeding Procedure 8 HHZ BC1F2 populations (08WS) DT screen SUB screen 15SUBT plants 326 Genotyping/progeny testing for all target traits Random plants 109DT plants Yield traits QTL/Allelic diversity discovery for target 82HY plants ST screen 120ST plants 326DT screen 311SUB screen326Yield 326ST screen 06WS 08WS 09DS Ist round selection 108Preliminary yield trials under DT, low input, NC Confirming genetic networks for target traits and their genetic relationships for target traits 68Promising ILs 47DT ILs 171SUB ILs73HY ILs 78ST ILs 09WS 369Genotyping/progeny testing for all target traits 10DS 10WS/11DS 68 Replicated yield trials ~80 promising ILs as parents for designed QTL pyramiding 2NCT & 29 MET for 11WS 3Demo 2nd round selection 3rd round selection Selections can be continued if certain lines segregating IRRIIRRIIRRIIRRIIRRIIRRIIRRIIRRI
  34. 34. GSR Introgression Breeding Procedure 8 HHZ BC1F2 populations (09WS) DT screen SUB screen 21SUBT plants 637Genotyping/progeny testing for all target traits Random plants 210DT plants Yield traits QTL/Allelic diversity discovery for target traits 119HY plants ST screen 287ST plants DT screen SUB screenYield under NC & LI ST screen 06WS 09WS 10DS 221SUB ILs Ist round selection 2nd round selection Confirming genetic networks for target traits and their genetic relationships 180DT ILs 221SUB ILs420HY&FUE ILs 44ST ILs 10WS 865Genotyping/progeny testing for all target traits 11DS ~80 promising ILs as parents for designed QTL pyramiding DT screen SUB screenYield under NC & LI ST screen DT ILs SUB ILsHY&FUE ILs ST ILs 136 PYT11WS 80 RYT12 DS 2 NCT & 11 MET 12DS 2 Demo selection 3rd round selection Selections can be continued if certain lines segregating
  35. 35. Target traitsTarget traitsTarget traitsTarget traits Number of ILsNumber of ILsNumber of ILsNumber of ILs Produced fromProduced fromProduced fromProduced from BNBNBNBN Selected at PYT &Selected at PYT &Selected at PYT &Selected at PYT & RYTRYTRYTRYT Nominated toNominated toNominated toNominated to MET & NCTMET & NCTMET & NCTMET & NCT Drought tolerance (DT)Drought tolerance (DT)Drought tolerance (DT)Drought tolerance (DT) 613613613613 79797979 21212121 High yield under lowHigh yield under lowHigh yield under lowHigh yield under low----input (LI)input (LI)input (LI)input (LI) 370370370370 27272727 3333 Salinity tolerance (SAL)Salinity tolerance (SAL)Salinity tolerance (SAL)Salinity tolerance (SAL) 502502502502 73737373 18181818 Submergence tolerance (SUB)Submergence tolerance (SUB)Submergence tolerance (SUB)Submergence tolerance (SUB) 128128128128 13131313 2222 High yield under irrigated (Y)High yield under irrigated (Y)High yield under irrigated (Y)High yield under irrigated (Y) 576576576576 100100100100 27272727 DT+LIDT+LIDT+LIDT+LI 246246246246 15151515 2222 DT+SALDT+SALDT+SALDT+SAL 326326326326 19191919 5555 DT+SUBDT+SUBDT+SUBDT+SUB 82828282 6666 DT+YDT+YDT+YDT+Y 382382382382 40404040 11111111 LI+SALLI+SALLI+SALLI+SAL 274274274274 10101010 1111 Multiple abiotic stress tolerant ILs developed from 16 donors into Huanghuazhan background and nominated to NCT using GSR breeding scheme.GenerationIRRIbredGSRlines StoodLI+SALLI+SALLI+SALLI+SAL 274274274274 10101010 1111 LI+SUBLI+SUBLI+SUBLI+SUB 38383838 0000 LI+YLI+YLI+YLI+Y 178178178178 1111 SAL+SUBSAL+SUBSAL+SUBSAL+SUB 60606060 9999 SAL+YSAL+YSAL+YSAL+Y 292292292292 42424242 8888 SUB+YSUB+YSUB+YSUB+Y 101101101101 5555 1111 DT+SAL+SUBDT+SAL+SUBDT+SAL+SUBDT+SAL+SUB 35353535 3333 2222 DT+SAL+YDT+SAL+YDT+SAL+YDT+SAL+Y 154154154154 9999 DT+SUB+YDT+SUB+YDT+SUB+YDT+SUB+Y 58585858 3333 LI+SAL+SUBLI+SAL+SUBLI+SAL+SUBLI+SAL+SUB 20202020 0000 LI+SAL+YLI+SAL+YLI+SAL+YLI+SAL+Y 117117117117 0000 LI+SUB+YLI+SUB+YLI+SUB+YLI+SUB+Y 36363636 0000 SAL+SUB+YSAL+SUB+YSAL+SUB+YSAL+SUB+Y 39393939 2222 total:total:total:total: 845845845845 146146146146 40404040 IL=Introgression lines; BN=Backcross Nursery;PYT=Preliminary Yield Trial;RYT=Replicated Yield Trial; NCT=National Cooperative Testing (Philippines); Multi-environment testing (IRRI) 2ndGenerationIRRIbredGSRlines Stood first in MET stage 2 under GRiSP GSR IR1-8-S6-S3-Y2
  36. 36. Yield in any given condition is a manifestation of several factors • Genotype • TPE • Superior plant ideotype to capture high photosynthetic efficiency into the relativephotosynthetic efficiency into the relative larger sink • Efficient photosynthates mobilization within plant system • Resistance to pests & diseases IRRIIRRIIRRIIRRIIRRIIRRIIRRIIRRI
  37. 37. Yield Potential : Highest possible grain yield obtainable under ideal set of conditions of inputs (water and nutrients) for a given ecology without insect pest, weed and diseases pressure GSR concept on grain yield potential:
  38. 38. GSR Yield Concept Maximize grain yield Increase yield potential Increase yield potential under Super Hybrids Super Rice Green Super Rice Maximize grain yield under limited supply of nutrients and water Increase yield potential under ample supply of nutrients and water under sufficient supply of nutrients and water IRRIIRRIIRRIIRRIIRRIIRRIIRRIIRRI
  39. 39. Yield barrier -TPE • High input environment • Moderate input environment • Low input environment Breeding strategy must capture genotypes for each conditions separately rather than identifying one genotype for all conditions IRRIIRRIIRRIIRRIIRRIIRRIIRRIIRRI
  40. 40. Yield Stability- Specific Selection strategy- Resilience Plant architecture designed for specific rice ecology Specific adaptation traits IRRIIRRIIRRIIRRIIRRIIRRIIRRIIRRI
  41. 41. GSR IR1-5-S14-S2-Y2
  42. 42. IRRI bred first generation of high yielding GSR materials 5.2 5.6 5.0 5.3 6.9 6.4 5.6 6.3 5.3 7.0 5.9 6.1 4.8 7.2 5.8 5.9 5.3 6.9 5.3 5.8 5.4 6.8 4.7 5.6 4.7 5.8 4.0 4.8 4.00 5.00 6.00 7.00 8.00 3-Season Data of HHZ Lines nominated to NCT 0.00 1.00 2.00 3.00 4.00 2010 WS 2011 DS 2011 WS Mean Seasonal Mean HHZ 12-SAL2-Y3-Y2 HHZ 8-SAL6-SAL3-Y2 HHZ 5-SAL6-SAL3-DT1 HHZ 5-SAL14-SAL2-Y2 NSIC Rc222 APO IRRIIRRIIRRIIRRIIRRIIRRIIRRIIRRI
  43. 43. IRRI bred GSR lines nominated to NCT Philippines
  44. 44. GSR IR1-8-S6-S3-Y2 High yielding under irrigated with moderate multiple abiotic stress tolerance GSR line with good cooking quality suitable for rainfed and irrigated lowlands.
  45. 45. Designation Grain Yield (t/ha) Mean over seasons % over IR72 % over NSIC Rc158 2010WS 2011DS HHZ8-SAL6-SAL3-Y2 6.55ab 8.0ab 7.28 10.56 12.27 Mestizo7 (Hybrid) 5.68 bcde 8.7a 7.19 9.27 10.96 HHZ12-DT10-SAL1-DT1 6.75a 7.2 bcde 6.98 6.00 7.64 IR83142-B-7-B-B 6.00 abcde 7.6 bc 6.80 3.34 4.94 HHZ5-SAL10-DT1-DT1 6.14abcd 7.4 bcd 6.77 2.89 4.48 IR72 5.96abcde 7.2 cde 6.58 0.00 1.54 Performance of IRRI bred GSR High Yield Potential Varieties under Irrigated Conditions HHZ5-DT8-DT1-Y1 5.55 cde 7.6 bc 6.58 -0.08 1.47 HHZ8-SAL12-Y2-DT1 6.43abc 6.7 def 6.57 -0.23 1.31 NSICRc158 5.86 bcde 7.1 cdef 6.48 -1.52 0.00 HHZ12-Y4-DT1-Y1 5.57cde 7.1 cdef 6.34 -3.72 -2.24 IR83142-B-19-B 5.12 e 7.5 bcd 6.31 -4.10 -2.62 IR83142-B-57-B 5.48 de 7.1 cdef 6.29 -4.41 -2.93 IR83143-B-21-B 5.16 e 7.2 cde 6.18 -6.08 -4.63 HHZ8-SAL9-DT2-Y1 5.78 bcde 6.4 defg 6.09 -7.45 -6.02 HHZ5-SAL10-DT3-Y2 5.69 bcde 6.3 fg 6.00 -8.89 -7.48 HHZ5-SAL10-DT2-DT1 5.47 de 6.0 g 5.74 -12.84 -11.50 Higher HI, spikelets per panicle;panicles per sqm;total spikelets per sqm, CGR Plot size: 30sqm SSNM High yielding, IG+DT+SAL+SUB+Low input SSNM IRRIIRRIIRRIIRRIIRRIIRRIIRRIIRRI
  46. 46. RYT IRRI 2011 WS Irrigated Low-input Rainfed BC1F8 HHZ 8-SAL6-SAL3-Y2 5.88 3.90 5.24 BC1F8 HHZ 8-SAL6-SAL3-Y1 4.41 2.99 3.46 BC1F7 HHZ 8-SAL6-SAL3- SAL1 3.95 3.00 4.38 Huang-Hua-Zhan 3.09 2.92 3.56 NSIC Rc222 4.71 2.95 5.17 Round 3 IRRI 2009 WS IG RF SAL SUB BC1F4 HHZ 8-SAL6-SAL3 4.40 4.25 10.00 55.56 IRRI bred GSR IR1-8-S6-S3-Y2 Breeding History Round 1 IRRI 2008 WS IG RF SAL SUB BC1F2 HHZ 8 9 SPS 13 SPS 36 SPS 0 SPS Round 2 IRRI 2009 DS IG DT SAL SUB BC1F3 HHZ 8-SAL6 5.58 2.25 41.67 36.36 >Promoted to MET2 (2012 DS) Combines earliness (105d), high yield inCombines earliness (105d), high yield in irrigated, low input, drought, salinity, submergence,irrigated, low input, drought, salinity, submergence, directdirect seeded, transplanted, resistant to blast, BLBseeded, transplanted, resistant to blast, BLB NSIC Rc222 4.71 2.95 5.17 Apo 3.98 3.47 3.60 MET1 2011 DS IRRI Philrice Philrice Philrice Los Banos CES Isabela Agusan BC1F7 HHZ 8-SAL6-SAL3-Y2 3.48 8.72 7.74 0.83 RYT IRRI 2011 DS Irrigated Low-input Drought BC1F7 HHZ 8-SAL6-SAL3-Y2 7.00 3.11 0.81 BC1F7 HHZ 8-SAL6-SAL3-Y1 6.05 2.75 0.86 BC1F6 HHZ 8-SAL6-SAL3- SAL1 5.90 2.52 0.95 Huang-Hua-Zhan 6.39 2.02 0.20 NSIC Rc222 6.77 2.93 0.46 Apo 5.78 2.88 1.35 PYT IRRI 2010 WS Irrigated Low-input Rainfed 75% N BC1F6 HHZ 8-SAL6-SAL3-Y2 5.32 4.17 5.32 5.09 BC1F6 HHZ 8-SAL6-SAL3-Y1 5.59 3.31 4.75 4.50 BC1F5 HHZ 8-SAL6-SAL3- SAL1 4.67 3.94 5.57 4.34 Huang-Hua-Zhan 4.89 4.20 4.42 5.37 NSIC Rc222 5.35 4.78 4.94 4.16 Apo 4.71 4.77 5.52 4.48 PYT IRRI 2010 DS Irrigated Low-input Drought BC1F5 HHZ 5 SAL14-SAL2-Y2 7.75 2.77 2.11 BC1F5 HHZ 8-SAL6-SAL3-Y1 6.66 1.94 2.45 Huang-Hua-Zhan 7.58 3.31 2.49 IR 72 6.99 3.08 2.53 Apo 6.94 3.23 3.07 > Submitted to NCT (Transplanted Rice Category) > Submitted to MET1 (2011 DS)
  47. 47. Mean yield (t/ha) of IRRI bred GSR entries in MET Stage 2, 2012DS MET Entry Fixed Name Location Ave. yieldNE Isabela Agusan Bohol IRRI MT4004 HHZ 8-SAL 6-SAL 3-Y2 8.86 7.20 4.32 5.20 5.97 6.31 MT4008 HHZ 1-Y4-Y1 8.22 7.21 3.74 4.81 4.97 5.79 MT4017 HHZ 8-SAL9-DT 2-Y1 7.52 6.39 3.94 4.75 5.03 5.53 MT4018 HHZ 12-Y 4-DT 1-Y 1 7.99 5.44 3.95 2.76 5.81 5.19 MT4019 HHZ 12-DT 10-SAL 1-DT 1 8.04 5.45 4.58 4.94 5.12 5.62 MT4901 IRRI 104 7.40 5.98 4.06 4.91 5.74 5.61 GSR IR1-8-S6-S3-Y2 = HHZ8-SAL6-SAL3-Y2 is irrigated highest yielder under DS; tolerant to drought(~ -75 kPascal at reproductive stage),salinity(6-10dSm1), submergence (14d submergence 4.3 PACP score). It is nutrient and radiation use efficient line IRRIIRRIIRRIIRRIIRRIIRRIIRRIIRRI Redona- MET Coordinator MT4901 IRRI 104 7.40 5.98 4.06 4.91 5.74 5.61 MT4904 IRRI 105 8.18 6.36 3.75 4.66 4.86 5.56 MT4902 IRRI 123 7.26 5.48 4.57 4.79 4.90 5.90 MT5211 IRRI 153 6.76 5.86 4.37 5.85 5.48 5.66 MT4903 IRRI 154 9.06 5.35 4.51 4.82 4.98 5.74
  48. 48. Rank Treatments means DMRT Groups Advantage over IR 63307-4B- 4-3 (ST Ck) Advantage over NSIC Rc184 (NCT-ST Ck) 1 HHZ 1-Y4-Y1 5.60 a 7.5% 18.0% 2 HHZ 5-SAL14-SAL2-Y2 5.38 ab 3.2% 13.3% 3 IR 63307-4B-4-3 (ST Ck) 5.21 abc 0.0% 9.8% 4 IR 83140-B-11-B 5.13 abc -1.5% 8.1% Salinity Trial at Infanta, Quezon(2012DS, Site EC ~6) 4 IR 83140-B-11-B 5.13 abc -1.5% 8.1% 5 HHZ 5-SAL8-DT3-SUB1 5.11 abc -2.0% 7.5% 6 HHZ 8-SAL6-SAL3-Y2 5.09 abc -2.4% 7.1% 7 HHZ 12-DT10-SAL1-DT1 5.06 abc -2.9% 6.6% 8 NSIC Rc184 (NCT-ST Ck) 4.75 bcd -8.9% 0.0% 9 IR 83140-B-28-B 4.65 cd -10.8% -2.1% 10 IR 84675-58-4-1-B-B 4.29 d -17.8% -9.7% 11 HHZ 5-Y3-SAL2-SUB1 4.19 d -19.6% -11.7% Seeding Date:20-Dec-11 3 replications Transplanting date:19-Jan-12 12.75sqm Fertilizer Rate:120-40-40 (N-P-K) IRRIIRRIIRRIIRRIIRRIIRRIIRRIIRRI
  49. 49. MULTIPLE ABIOTIC TOLERANT IRRI BRED GSR LINES SCREENED FOR SUBMERGENCE TOLERANCE Independently screened By BERT COLLARD during DS2012 in submergence tanks at IRRI Checks average over 8-10 reps while all other lines averaged over three replications Triple traits like DT-SUB-SALDT-SUB-SAL combination-highlighted in orange; RED highlighted arrow has the mean highest yielder over DS&WS W142 highlighted in blue is in NPT background and has AGT+SUBT+SAL and moderate DT
  50. 50. 2.00 4.00 6.00 8.00 Victoria Yieldt/ha PVS-GSR trials in Laguna province, Philippines harvested yield (t/ha) DS2012 (harvested plot size ~700sqm and twice replicated) GSR IR1-8-S6-S3-Y2 gave mean of 26.4% advantage over NSIC222 at two PVS sites tested in Laguna 0.00 2.00 Victoria Bae Cultivar Mean Yieldt/ha IRRIIRRIIRRIIRRIIRRIIRRIIRRIIRRI
  51. 51. Rapid Visco Analyzer (RVA) Pasting properties of GSR lines in IR64 and HHZ RP backgrounds- suitable for varied consumers with different taste preferences 3000 4000 5000 6000 Viscosity,cP 60 80 100 120 Temperature 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 -1000 0 1000 2000 0 100 200 300 400 500 600 700 800 Time, sec Viscosity,cP 0 20 40 Temperature 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 AC=14.5-31.6%; GT=H-I-L; Protein=7.8-11.2
  52. 52. D GSR IR1-12-D10-S1-D1 AROMATIC-Suitable -Direct seeding & Irrigated conditions, good cooking quality (AC24%,GT-L, Protein 9.4%) Drought and Salinity tolerant
  53. 53. GSR IR1-12-D10-S1-D1 PVS trials (40 farmers) at Puypuy, Laguna –ranked best over farmer’s check NSiC214 during WS2011 with preference score=0.118 against - 0.0063(NSiC214) High Yielding, Suitable for Rainfed & Irrigated lowland conditions, Aromatic, good cooking quality (AC 24%, GT-L, Protein 9.4%) with moderate tolerance to Salinity
  54. 54. Selection Strategy Favors Resilience Conventional breeding strategy IRRI-GSR breeding strategy IRRIIRRIIRRIIRRIIRRIIRRIIRRIIRRI
  55. 55. ResilientResilient GSR products
  56. 56. No. Populat ion No. of ILs HY LI DT RF ST SubT 1 HHZ 5 13 4 6 3 2 7 5 2 HHZ 8 6 2 2 0 3 5 2 3 HHZ 9 1 0 0 0 0 0 1 Number of HHZ ILs according to traits. 3 HHZ 9 1 0 0 0 0 0 1 4 HHZ 11 4 0 1 1 0 2 3 5 HHZ 12 6 3 3 4 0 1 0 6 HHZ 15 1 0 1 0 0 1 0 6 HHZ 17 3 0 1 0 0 2 0 Total: 34 9 14 8 5 18 11
  57. 57. Criteria for DQP Best HHZ ILs from Batch 1 for Design QTL Pyramiding (DQP) •Lines that performed well in at least 2 season from 4 seasons (2010DS, 2010WS, 2011DS, & 2011WS) of testing for irrigated (HY), low-input (LI), drought (DT), and Rainfed (RF) conditions;(LI), drought (DT), and Rainfed (RF) conditions; •Salinity tolerant and submergence tolerant lines in second round of screening (BC1F3, 2009DS) that has been confirmed in third round of screening (BC1F4, 2009WS).
  58. 58. No. Populat ion No. of ILs 1 trait 2 traits 3 traits 4 traits 5 traits 1 HHZ 5 13 5 5 1 2 0 2 HHZ 8 6 4 0 0 1 1 3 HHZ 9 1 1 0 0 0 0 Number of HHZ ILs with multiple traits 3 HHZ 9 1 1 0 0 0 0 4 HHZ 11 4 2 1 1 0 0 5 HHZ 12 6 1 5 0 0 0 6 HHZ 15 1 0 1 0 0 0 7 HHZ 17 3 3 0 0 0 0 Total: 34 16 12 2 3 1
  59. 59. IL2- HY, DT, STIL1- HY, DT, SUB X F1 F2 DQP strategy for combining high yield(HY), drought tolerance(DT), salinity tolerance(ST) and submergence tolerance(SUBT) using the promising ILs as parental lines Designed QTL pyramiding (DQP) DT screen SUB screen SUBT plants SNP Genotyping/progeny testing for all target traits Random plants DT plants Yield traits Group genotypes for different traits and their NILs HY plants ST screen ST plants New HYVs cultivars with DT,ST, SUBT for NCYT and PVS Promising PLs for yield Confirm genetic networks for different target traits and their genetic relationship Farmers
  60. 60. GSR Technology GSR Technology ILBreeding, DQP & PDLs Ideal RP BG Ecosystem based approach GSR 500 donors 56 RPs Screening of released GSR materials under target ecosystems Screening of already developed PDLs for abiotic stresses DT, ST, SUB, LI in the target ecosystems DQP for a trait & ecosystem related traits ILs, PDLs, DQP with adaptable RP BG for different target ecosystem Increase in success rate to develop highly adaptable genotypes for a given ecosystem First Phase 2009-2012 Second Phase 2012-2018 Ecosystem based approach
  61. 61. An additional ton of rice in the rain-fed and irrigated lowlands will change the livelihoods of millions of resource poor farmers from the clutches of poverty and sustained income source to prosper…. THANKS GSR IR1-12-D10-S1-D1 –Aromatic & High Yielding Suitable for Direct seeding & Irrigated conditions, good cooking quality (AC24%,GT-L, Protein 9.4%) + Drought and Salinity tolerant
  62. 62. Acknowledgements & Thanks: IRRI-CAAS-BMGF Dr Zhikang Li Director GSR project GSR National Coordinators (Asia) Drs Tuat, Untung, Helaluddin, Somphet, Nimal, Riaz, Arif, Makara, Two public/NGO sectors: Dr W.Xu (Boshima-SS,IDO) & Dr Sirajul Islam(BRAC) Dr C.X Mao GSR Training Consultant (CAAS-GAAS)Dr C.X Mao GSR Training Consultant (CAAS-GAAS) GSR-CAAS team: Drs Z. Li, Gao, Xu, Judy, Fu, Yu & others IRRI GSR team: Drs Nollie, Glenn, Choi, Redonna,Val Pede,Andy,Krishna,Wang,Tao GSR-MBL team: Gelo; Corine, Lolit, Denver, Macdo, Cenon, Keno, Deo, Eugene Visiting Research Fellows: Drs Ma, PhD: Zilhas, Meng; MS:Nina OJT: Shahana, Dilruba GSR Project Adm.: Pauline; Secretarial Assistance: Badett “Cooperation & Collaboration makes the world a smaller place”

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