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Infascelli final

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Findings requiring explanation in some GMO papers

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Infascelli final

  1. 1. Infascelli et al.
  2. 2. Document list 1. V. Mastellone, “Destino ed effetti metabolici di DNA transgenico in conigli alimentati con soia geneticamente modificata,” 2006, PhD Thesis. 2. R. Tudisco, P. Lombardi, F. Bovera, D. DˇAngelo, M. I. Cutrignelli, V. Mastellone, V. Terzi, L. Avallone, and F. Infascelli, “Genetically modified soya bean in rabbit feeding: detection of DNA fragments and evaluation of metabolic effects by enzymatic analysis,” Anim. Sci., vol. 82, no. 02, pp. 193–199, 2006. 3. R. Tudisco, F. Infascelli, M. I. Cutrignelli, F. Bovera, C. Morcia, P. Faccioli, and V. Terzi, “Fate of feed plant DNA monitored in water buffalo (Bubalus bubalis) and rabbit (Oryctolagus cuniculus),” Livest. Sci., vol. 105, no. 1–3, pp. 12–18, Dec. 2006. 4. R. Tudisco, M. I. Cutrignelli, S. Calabrò, A. Guglielmelli, and F. Infascelli, “Investigation on genetically modified soybean (RoundUp Ready) in goat nutrition: DNA detection in suckling kids,” Ital. J. Anim. Sci., vol. 6, no. SUPPL. 1, pp. 380–382, 2007. 5. R. Tudisco, S. Calabrò, F. Bovera, M. I. Cutrignelli, A. Nizza, V. Piccolo, and F. Infascelli, “Detection of plant species-specific dna (barleyand soybean) in blood muscle tissue, organs and gastrointestinal contents of rabbit,” World Rabbit Sci., vol. 18, no. 2, pp. 83–90, 2010. 6. R. Tudisco, V. Mastellone, M. I. Cutrignelli, P. Lombardi, F. Bovera, N. Mirabella, G. Piccolo, S. Calabrò, L. Avallone, and F. Infascelli, “Fate of transgenic DNA and evaluation of metabolic effects in goats fed genetically modified soybean and in their offsprings,” Animal, vol. 4, no. 10, pp. 1662–1671, 2010. 7. V. Mastellone, R. Tudisco, G. Monastra, M. E. Pero, S. Calabrò, P. Lombardi, M. Grossi, M. I. Cutrignelli, L. Avallone, and F. Infascelli, “Gamma- Glutamyl Transferase Activity in Kids Born from Goats Fed Genetically Modified Soybean,” Food Nutr. Sci., vol. 2013, no. June, pp. 50–54, 2013. 8. R. Tudisco, S. Calabrò, M. I. Cutrignelli, G. Moniello, M. Grossi, V. Mastellone, P. Lombardi, M. E. Pero, and F. Infascelli, “Genetically modified soybean in a goat diet: Influence on kid performance,” Small Rumin. Res., vol. 12, pp. 1–8, 2015.
  3. 3. Tudisco et al., Animal Science, 2006
  4. 4. Animal Science, 2006 – fig2 and fig4 Figure 4. Figure 2. Deletion
  5. 5. Animal Science, 2006 – fig2 source Fig 2 in Animal Science 2006 was obtained by cropping lanes 3-13 and the controls from the Thesis image (look for features outside the bands). SAMPLES DO NOT MATCH. E.G.: lane 4 in the Thesis is heart, but in the paper is muscle; lane 6 in the Thesis is kidney, in the paper is heart. Mastellone’s PhD Thesis (2006) Fig 17, contrasted. There are several deleted regions in this image.
  6. 6. Animal Science, 2006 – fig3 Controls appear sliced-in (high contrast version) Original, fig. 3
  7. 7. Fig 4 in Animal Science 2006 appears to be completely fabricated starting from figure 17 in Mastellone’s thesis. IF CONFIRMED, PRIMERS DO NOT MATCH. Animal Science, 2006 – fig4 source
  8. 8. R. Tudisco, F. Infascelli, M. I. Cutrignelli, F. Bovera, C. Morcia, P. Faccioli, and V. Terzi, “Fate of feed plant DNA monitored in water buffalo (Bubalus bubalis) and rabbit (Oryctolagus cuniculus),” Livest. Sci., vol. 105, no. 1–3, pp. 12–18, Dec. 2006
  9. 9. Mastellone’s PhD Thesis (2006) Fig 14, unmodified Lane 9 spliced in. The same image is used to produce Thesis images 15 and 16, cropping and moving the lanes in different positions. Figure 3 for the paper in question derived from fig.14 in Mastellone Thesis. Please note that the same figure resurfaces again in Tudisco 2007 It.J.Anim.Sci (as source for fig 1 & 2, with changed species) and in Tudisco 2010 World Rabbit Science (fig 2) with changed labels (e.g. kidney becomes liver)
  10. 10. It.J.Anim.Sci, 2007
  11. 11. It.J.Anim.Sci, 2007 – Fig. 1 and 2 duplication Figure 1 Figure 2
  12. 12. It.J.Anim.Sci, 2007 – Fig. 1 and 2 origin Mastellone’s PhD Thesis (2006) Fig 14 (lane 9 spliced in as previously discussed) FIG 1 & 2, left panel: evidence for internal lane duplications (red). Controls and some lanes are derived from vertically stretching the corresponding lanes in Mastellone Thesis, fig. 14 (red, blue and green). Please note that the original figure refers to Bubalus sp., while the figure in the paper refers to goat.
  13. 13. R. Tudisco, S. Calabrò, F. Bovera, M. I. Cutrignelli, A. Nizza, V. Piccolo, and F. Infascelli, “Detection of plant species-specific dna (barleyand soybean) in blood muscle tissue, organs and gastrointestinal contents of rabbit,” World Rabbit Sci., vol. 18, no. 2, pp. 83– 90, 2010.
  14. 14. World Rabbit Sci., 2010 Mastellone’s PhD Thesis (2006) Fig 14, unmodified Lane 9 spliced in. The same image is used to produce Thesis images 15 and 16, cropping and moving the lanes in different positions. Figure 2 for the paper in question derived from fig.14 in Mastellone Thesis but labels change (e.g. kidney becomes liver). Lanes D, C and F are fabricated by cloning different portions of lanes K, S & NTC (see red boxes and arrows). A band was deleted in lane NTC (yellow arrow) Please note that the same figure was used also in Tudisco Livest. Sci. 2006 and in Tudisco It.J.Anim.Sci, 2007 (as source for fig 1 & 2, with changed species)
  15. 15. OTHER FINDINGS
  16. 16. Image sharing portions Animal 2010, fig.2 right panel Small Rum Res 2015, fig.2 left panel
  17. 17. Animal, 2010 – Figure 1 – Small ruminant Res., 2015 - left panel. Figure 1 - Image reuse The negative control for the experiment on the left is manipulated. There is clear evidence for a software deletion of the original signal. The manipulated image is reused! Milk and blood Colostrum deletion
  18. 18. Animal 2010 FoodNutrSci 2013 Figure 4. Figure 3. Panel a: lanes 1-5, treated; lanes 6-10, controls Panel b: lanes 1-5, controls; lanes 6-10, treated. Moreover, please note the change of 2010 lane 6 into a 2013 negative control Figure 1. Panel b: lanes 1-6, treated; lanes 7-12, untreated Panel d: lanes 1-6, treated; lanes 7-12, untreated Fig.3 and fig4 may share the red portion if the corresponding panel are from a single gel. However, fig.3 shows sign of splicing-in for the red portion. High resolution material is needed to check for this. Small Rum Res 2015, control reuse ColostrumMilk and blood Figure 2.

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