2. Presentation outline
• Pathophysiology of hematopoiesis
• Definition of anemia
• Classification
• General approach to anemic patient
• Discussion of commonest types of anemia with respective treatment
3. Pathophysiology of hematopoiesis
Hematopoiesis- the process by which circulating blood cells are formed
in bone marrow.
it is estimated that 1010 erythrocytes and 108 to 109 leukocytes are
produced per hour in the steady state.
they can be greatly amplified on demand.
These huge cell numbers are immediate descendants of maturing
precursors that arise from a smaller pool of progenitors.
The progenitors in turn arise from an even smaller pool of
hematopoietic stem cells (HSC)
4. hematopoietic stem cells (HSC)- are thought to be mostly in a resting or
nondividing state and have the capacity to self-renew (and thus maintain
their numbers).
Hematopoietic stem cells are multipotent and have the capacity to
differentiate into the cells of all 10 blood lineages — erythrocytes, platelets,
neutrophils, eosinophils, basophils, monocytes, T and B lymphocytes, natural
killer cells, and dendritic cells
8. Anemia
Pathophysiological definition:
Reduction in red cell mass from circulation
Laboratory definition:
Reduction in haemoglobin concentration below normal
WHO criteria: Males Hgb<13 gm/dl,
Females Hgb<12gm/dl
9. classification
• Pathophysiologic
• Hypoproliferetive
• Hemolytic anemia
• Anemia of blood lose
• RBC Morphology
• Normocytic normochromic- acute blood lose, anemia of chronic illness(70%)
• Microcytic hypochromic:- e.g iron deficiency, lead poisoning
• Macrocytic anemia:- folat and cobalamin deficiency, drug like zidovudine
10. 1. Hypoproliferative anemia
Impaired red cell production due to luck of substrate or impaired bon marrow
function by infiltration
Iron deficiency anaemia
.Anaemia of chronic disease
Bone marrow failure syndromes: BM infiltration withleukemia, lymphoma,
metastatic malignancy and
Metabolic and endocrine failure syndromes: hypothyroidism,
hypopituitarism; malnutrition
Vitamin b12 0r B9 deficiency
Drugs ;- zidovudine’ ,mthotrixate
12. 3. Animia of blood lose
• Any obvious bleeding or occult bleeding
13. • Normocytic normochromic anemia
– Anemia of chronic illness (70% of the cases)
– CKD
– Early iron deficiency anemia
– Acute blood loss
• Microcytic hypochromic anemia
– Iron deficiency
– Lead poisoning
– Sideroblastic anemia
– Thalassemia
– Anemia of chronic illness (30% of the cases)
15. Approch to the pt
• History
• physical examination
• Diagnostic invstigation
16. Iron deficiency anemia
The most common nutritional disorder world wide
Globally, 50% of anemia is attributable to iron deficiency
Prevalence higher in developing countries
Africa and Asia bear 71% of the global mortality burden due to iron deficiency
anemia
Common also in US-toddlers, adolescent girls
17. Iron metabolism
The normal diet of western diet contains-10-20mg per day
The total body iron content is normally about 2gm in women & 6 gm in men
It is divided into functional & storage comportment
About 80% of functional found in Hb, myoglobin and enzymes
The storage pool is represented by ferritin & hemosiderin-15-20% of total
body
18. • So total iron binding capacity of serum is 300-350µg/dL
• Most Fe is absorbed in duodenum through two distinct
pathways
• Since body loss of Fe is limited, Fe balance is maintained by
regulating absorptive intake
• Mechanisms are still incompletely understood
19. Etiology
Decreased intake
Rare in industrialized countries(Can occur in infants, children, elderly)
Increased demand
Growing infants, children and adolescents
during pregnancy
Impaired absorption
Celiac disease,Tropical sprue
Gasterectomy
Chronic blood loss
Commonest cause in western
20. Stages in the development of IDA
1. Depletion of iron stores
Decreased level of serum ferritin (<20µg/L) and BM iron stain 0 to1+
NB: serum ferritin of 50-200µg/L and marrow iron store of 1-3 + is
expected in normal state
Normal level of SI, TIBC,% saturation, RBC protoporphyrin level
Pt not symptomatic /not anemic
21. 2. Iron deficient erythropoiesis
Serum ferritin <15µg/L and BM iron stain 0
Reduced SI (<50 µg/dl) and % saturation (<20%)
Increased TIBC (>360 µg/dl ) and RBC protoporphyrin level (>100
µg/dl)
24. Clinical prentation
• Non specific Sx and Sn of anemia
• In addition to the anemia other changes can occur with severe
deficiency – koilonychia, alopecia, atrophic changes in tongue &
gastric mucosa and intestinal malabsorption
• The dominating Sn and Sx relate to the underlying cause
25. Morphology
In peripheral smear RBCs are
Small (microcytic) &
Pale (hypochromic)
Poikilocytosis in the form of small elongated red cells (pencil cells) are characteristic
In bone marrow,
Erythroid hyperplasia
The disappearance of stainable iron from mononuclear phagocytic cells is a
diagnostic finding-Prussian blue st
29. treatment
• Red cell transfusion- indications
– Cardiovascular instability
– HCT <15%
– Continued and excessive blood loss
– Patients requiring immediate intervention
• Oral iron therapy
– 300mg of elemental iron per day
– Ideally, should be taken on empty stomach
– Should continue for a period of 6-12 months after correction of the anemia
– Response- the reticulocyte count begin to increase after 4-7days of therapy and peak at 10
days
– S/E include abdominal pain, nausea, vomiting, and constipation
30. • Parental iron therapy- indicated for patients
– Who are unable to tolerate oral iron
– Whose needs are relatively acute
– Who need iron on an ongoing basis, usually due to persistent GI blood loss
– Taking EPO
• Preparations include
– Iron dextran
– Ferric gluconate
• Feared complication- anaphylaxis, especially with iron dextran infusion
– Anaphylaxis is much rarer with the newer preparations
31. Megaloblastic anemia
• Two principal types
1. Cobalamin deficiency anemia
2. Folate deficiency anemia
• It results in defect in DNA synthesis which lead to
Enlargement of erythroid precursors (megaloblast)
Large cells in peripheral blood (macrocytes)
Hypersegmented neutrophils
• The precise basis for the changes is not fully understood
32. • Vitamin B12 and Folate are coenzymes required for synthesis of thymidine
• Deficiency or impairment in their metabolism results in defective nuclear
maturation
• Delay or block cell division
• The synthesis of RNA and protein is relatively unaffected
• Cytoplasmic maturation proceeds in advance of nuclear maturation-
Nuclear/Cytoplasmic asynchrony
33. morphology
Certain morphologic features are common to all
Pancytopenia
Marked variation in size and shape of red cells(Anisopoikliocytosis)
RBC-macrocytic & oval(Macro-ovalocytes)
MCV > 100fl
Reticulocyte count is low
Nucleated red cells occasionally
35. • Granulocytes precursors also display nuclear/cytoplasm
asynchrony
• Megakaryocytes-Abnormally large and have bizarre, multilobed
nuclei
• The anemia is further exacerbated by increased hemolytic
destruction
• Mild to moderate Fe overload
37. Vit B12 metabolism
• Human are totally dependent on diet
• Daily requirement-1-3µg.
• Absorption require intrinsic factor
• Vit B12 freed from binding protein by pepsin
• Free Vit B12 binds to cobalophilins(R-binder)
• This complexes are broken down by pancreatic protease
• Vit B12 then associates with intrinsic factor
• Endocytosis
• Binding with transcobalamin II
39. Clinical feature
The same as other type of anemia
CNS manifestations
• Found in 3/4th patients
• The spinal cord
Degeneration of myelin in the dorsal and lateral tract
Sometimes followed by loss of axon
• Degenerative change in the ganglia of posterior roots and peripheral nerves
• Psychiatric features are also seen in some patients with cobalamin or folate
deficiencies
40. Folate deficency
• A deficiency of folic acid result in megaloblastic anemia
• Have the same characteristic as vit B12 but no neurologic change
• Depressed synthesis of DNA is the immediate cause
41. • Diagnosis can be made only by demonstration of decreased
folate levels in the serum or red cells
• Elevated homocystein with normal MMA level
• Hematologic symptoms of vit B12 deficiency anemia respond to
Folate therapy
• But doesn’t prevent the progression of neurologic deficits ( May
even exacerbate
42. Treatment of megaloblastic anemia
• Cobalamin deficiency
– Lifelong regular cobalamin injections
– Hydroxycobalamin or cyanocobalamin can be used
– Hydroxycobalamin 1000µg IM weekly for 6 wks followed by 1000µg IM every 3 months lifelong
• Folate deficiency
– Folic acid 5-15 mg po/day
– Rule out cobalamin deficiency before initiating folic acid
– Duration of treatment depends on the underlying cause
• Prophylactic folic acid is indicated during
– Pregnancy
– Premature infants
– Chronic dialysis patients
– Patients on total parentral nutrition