Dr.Elza Joy Munjely
Depmt. Of Pharmacology
Govt. Medical College,Kottayam.
INTRODUCTION TO PROTEIN
• Protein synthesis inhibitor - substance that stops or
slows the growth or proliferation of bacterial cells by
disrupting the processes of generation of new proteins by
targeting the bacterial ribosome.
• Act at the ribosome level
• Take advantage of the major differences between
prokaryotic and eukaryotic ribosome.
• Work at different stages of mRNA translation like
initiation, elongation, and termination.
MOA OF AMINOGLYCOSIDES &
Penetrate bacterial cell wall through porin channels by
Betalactams facilitate passive diffusion of
Bind to 30S ribosomal subunit
Improper formation of initiation complex
Misreading of mRNA
IMPROPER FORMATION OF INITIATION
COMPLEX & MISREADING OF mRNA
MOA OF LINEZOLID
Binds to 50S ribosomal subunit near the interface with
Prevents the formation of ribosomal complex.
INHIBITION OF RIBOSOMAL COMPLEX
MOA OF TETRACYCLINES
Drug enter into cytoplasm by:
Energy dependent tetracycline active transport system –
Gram positive bacteria
Passive diffusion through porin channels – Gram
Binds to 30Ssubunit of the ribosome
& inhibits the binding of aminoacyl tRNA to the A site.
PREVENTS BINDING OF AMINOACYL tRNA
TO A SITE
MOA OF CHLORAMPHENICOL
Binds to 50S subunit of the ribosome
Inhibits peptide bond formation.