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Hoekman ecn 2012

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Hoekman ecn 2012

  1. 1. Image courtesy of People Productions Design, sampling and storage of the NEON terrestrial insect collections Dr. David Hoekman • Insect Ecologist • National Ecological Observatory Network • dhoekman@neoninc.org
  2. 2. Chironomus islandicus over Mývatn, May 2008 Photo: Árni Einarsson
  3. 3. NEON GoalThe goal of NEON is to enable understanding and forecasting of the impacts of climate change, land use change and invasive species on continental-scale ecology by providing data and infrastructure to support research, education and environmental management in these areas.
  4. 4. Systematic, standardized sampling across sites
  5. 5. NEON Observing Systems • Terrestrial observing system (organismal biology) • Terrestrial instrument system (climate, biogeochemistry, soils) • Aquatic observing system (organismal and instrumental) • Airborne observing system (remote sensing) • STREON (top-down and bottom-up controls, aquatic) • Mobile deployable platform • Land use analysis package (integration with national data • NEON collections
  6. 6. The first NEON tower in Sterling, Colorado
  7. 7. A CONTINENTAL-SCALE OBSERVATION SYSTEM
  8. 8. The National Ecological Observatory will provide access to: • All data* • All metadata* • All QA/QC information (process and data) • Protocols and procedures used to collect data • Instrument specifications, characteristics and performance • Algorithms used to process data *Unless legally protected by the Endangered Species Act or other legislation NEON Access Policy
  9. 9. Organismal Sampling
  10. 10. • Plant biodiversity • Plant biomass, leaf area, and chemical composition • Plant phenology • Birds • Ground beetles • Mosquitoes • Small mammals • Disease • Soil microorganisms • Soil biogeochemistry Terrestrial Observation System (TOS)
  11. 11. TOS – Site Specific Sampling Design • Spatially balanced design • Stratified by vegetation type • Insects sampled at biodiversity plots
  12. 12. Terrestrial Biological Field Sampling Draft site schematic for TOS sampling
  13. 13. Ground beetle sampling Carabidae • Charged with measuring biodiversity and abundance • Weekly pitfall samples from each site during the field season • 4 pitfalls at 10 biodiversity plots for 40 traps per site • Pinned/pointed • identified using reference collection, expert taxonomists, DNA barcoding • “by-catch”
  14. 14. Culicidae • Charged with measuring biodiversity, abundance and phenology • Mosquitoes also being sampled for disease • Weekly CDC light-traps (CO2 baited) at the core site, every 4 weeks at relocatable sites at 10 biodiversity plots per site • Identified using reference collection, expert taxonomists, DNA barcoding • Off-season vs. field season for phenology Mosquito sampling
  15. 15. NEON – Generated Natural History Collections Use existing specimens for DNA barcodes (Construction) • Validation of field and lab identifications of sentinel taxa Curation of NEON samples (Operations) • Voucher collections of sentinel taxa – For external PI-driven research needs – Long-term storage • Range of organisms • Insects • Vascular plants and algae • Animal tissues and genomic extracts • Microbial communities • Soils and sediments
  16. 16. DNA barcoding • Cara’s recent publication • Integrative Taxonomy for Continental-scale Terrestrial Insect Observations - PLoS ONE 7(5): 2012 e37528.doi:10.1371/journal.pone.0037528 • Currently contracting with the Canadian Centre for DNA Barcoding • all of our records are added to the Barcode of Life Database (BOLD) • We will continue to barcode beetles and mosquitoes from each site/domain as we build up our reference collection
  17. 17. Standing up the observatory 2013 2013 2013

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