Intracytoplasmic Sperm Injection - ICSI


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Intracytoplasmic Sperm Injection - ICSI. Indications, the procedure - ICSI photos - Methods of sperm retrieval which are used for ICSI

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Intracytoplasmic Sperm Injection - ICSI

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  3. 3. Specialized tools and inverted microscopes Pick up a sperm using a specialized ICSI needle Advanced through the egg membrane The sperm is injected into the inner part of the egg (the cytoplasm). Then fertilization occurs in 75 - 85% of eggs injected with sperm.
  4. 4. Male infertility factors that includes low sperm counts, poor motility or movement of the sperm, poor sperm quality, sperm that lack the ability to penetrate an egg or azoospermia. Failure of fertilization in previous IVF attempts. If the male partner underwent a vasectomy and sperm have been collected from the testicles or epididymis. If male partner has problems in erection or ejaculation. It is used for couples with a small number of eggs at egg retrieval, to get a higher percentage of eggs fertilized than when mixing eggs and sperms together in the IVF procedure.
  5. 5.  Stimulation Of Ovaries  Collection Of Eggs by hollow needle & ultrasound guidance.  Sperm Picking by delicate needle.  Transferred to the uterus 2 to 3 days after fertilization.  Pregnancy test should be done 2 weeks after the embryo transfer.
  6. 6. Methods of sperm retrieval for I.C.S.I  If there were sperm in the semen, a sperm sample can be produced by normal ejaculation into a cup on the same day of egg collection.  If there is no sperm in the semen, the doctor can extract sperm from the male partner under local or general anesthesia. Percutaneous Epididymal Sperm Aspiration & I C S I (P.E.S.A , M.E.S.A) It is only required if the husband has one of the following problems:  Absence of the vas deferens (through which the sperm passes from the epididymis to the seminal fluid ).  Past Infections that result in obstruction.  Cases who have had a vasectomy and surgical reversal failed.
  7. 7. Testicular Sperm Aspiration & I.C.S.I (T.E.S.A, T.E.S.E)  Obtaining sperm directly from the testes, either by aspiration, using a needle or surgical extraction  The sperm are then used to do the ICSI procedure as described above. This method is used in the following cases:  Absence of sperms in the epididymis.  Absent or obstructed epididymis.  TESE might be done before the beginning of the treatment cycle and the collected sperm are then frozen.
  8. 8.  The risk of multiple births and ectopic pregnancy.  The risk of congenital abnormalities.  There is an increased risk of genetic problem carried by the sperm.  ICSI is more expensive than IVF.
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