Sentinel Lymph Node Biopsy (SLNB) in Malignant Melanoma www. oncopathology.blogspot. com
www.Oncopathology.blogspot.com Melanoma Risk Factors
www.Oncopathology.blogspot.com Melanoma Risk Factors
www.Oncopathology.blogspot.com Malignant Melanoma
www.Oncopathology.blogspot.com Malignant Melanoma
www.Oncopathology.blogspot.com Malignat Melanoma
Malignant Melanoma Prognostic factors . www.Oncopathology.blogspot.com
Malignant Melanoma
www.Oncopathology.blogspot.com Malignant Melanoma
www.Oncopathology.blogspot.com Malignant Melanoma
www.Oncopathology.blogspot.com Malignant Melanoma
www.Oncopathology.blogspot.com Malignant Melanoma
Sentinel Lymph node procedure  in  Malignant Melanoma www.Oncopathology.blogspot.com
www.Oncopathology.blogspot.com Malignant Melanoma
Processing of Sentinel Lymph node in malignant melanoma <ul><li>Cochran Method: </li></ul><ul><li>As majority   of metasta...
Sentinel Lymph node for melanoma Pathology Processing protocol www.Oncopathology.blogspot.com <ul><li>The lymph node is ei...
Cook, M G et al. J Clin Pathol 2008;61:897-902 Metastases of melanoma are said to occur close to the median plane of the s...
<ul><li>The alternative technique recommended by Starz   et al </li></ul><ul><li>Consists of slicing the SLN at 2 mm inter...
S-100 immunostaining of a sentinel lymph node demonstrating metastatic cells in a subcapsular sinuse. HMB-45 staining show...
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Melanoma Sentinel Lymph node

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Melanoma Sentinel Lymph node

  1. 1. Sentinel Lymph Node Biopsy (SLNB) in Malignant Melanoma www. oncopathology.blogspot. com
  2. 2. www.Oncopathology.blogspot.com Melanoma Risk Factors
  3. 3. www.Oncopathology.blogspot.com Melanoma Risk Factors
  4. 4. www.Oncopathology.blogspot.com Malignant Melanoma
  5. 5. www.Oncopathology.blogspot.com Malignant Melanoma
  6. 6. www.Oncopathology.blogspot.com Malignat Melanoma
  7. 7. Malignant Melanoma Prognostic factors . www.Oncopathology.blogspot.com
  8. 8. Malignant Melanoma
  9. 9. www.Oncopathology.blogspot.com Malignant Melanoma
  10. 10. www.Oncopathology.blogspot.com Malignant Melanoma
  11. 11. www.Oncopathology.blogspot.com Malignant Melanoma
  12. 12. www.Oncopathology.blogspot.com Malignant Melanoma
  13. 13. Sentinel Lymph node procedure in Malignant Melanoma www.Oncopathology.blogspot.com
  14. 14. www.Oncopathology.blogspot.com Malignant Melanoma
  15. 15. Processing of Sentinel Lymph node in malignant melanoma <ul><li>Cochran Method: </li></ul><ul><li>As majority of metastases are in a central plane, the bivalve technique </li></ul><ul><li>in which a slice is made through the convex capsule and the hilum, reveals the maximum cut surface </li></ul>www.Oncopathology.blogspot.com
  16. 16. Sentinel Lymph node for melanoma Pathology Processing protocol www.Oncopathology.blogspot.com <ul><li>The lymph node is either bivalved or cut into 3 mm blocks, depending on the size of the node. </li></ul><ul><li>Sections 1, 3, and 5 are stained with haematoxylin and eosin (H&E) </li></ul><ul><li>Sections 2 and 4 are immunohistochemically stained for S-100 and HMB-45 </li></ul>
  17. 17. Cook, M G et al. J Clin Pathol 2008;61:897-902 Metastases of melanoma are said to occur close to the median plane of the sentinel lymph node and therefore bivalving, as shown, and embedding face down in the cassette are recommended. www.Oncopathology.blogspot.com Processing of Sentinel Lymph node in malignant melanoma
  18. 18. <ul><li>The alternative technique recommended by Starz et al </li></ul><ul><li>Consists of slicing the SLN at 2 mm intervals. </li></ul><ul><li>It is attractive in that it does not depend on the slightly difficult identification of a central plane </li></ul><ul><li>It does not require multiple sections on each slice. </li></ul><ul><li>In larger nodes, there may be a need for several blocks. </li></ul>Processing of Sentinel Lymph node in Malignant Melanoma www.Oncopathology.blogspot.com
  19. 19. S-100 immunostaining of a sentinel lymph node demonstrating metastatic cells in a subcapsular sinuse. HMB-45 staining showing metastatic melanoma cells within subcapsular sinuses of a sentinel lymph node. www.Oncopathology.blogspot.com Sentinel Lymph node in Malignant Melanoma

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