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Effect of DDGS, enzyme supplementation and necrotic enteritis on broiler chickens


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Effect of DDGS, enzyme supplementation and necrotic enteritis on broiler chickens

  1. 1. The effect of high dietary inclusion of DDGS, enzyme supplementation and necrotic enteritis challenge on gut microflora and immune response of broiler chickens M.R. Barekatain1, C. Antipatis2, S.W. Walkden-Brown1, P.A. Iji1, and M. Choct3 1- School of Environmental and Rural Science, University of New England, Armidale NSW, Australia 2- DSM Nutritional Products Asia Pacific Pty Ltd. 2 Havelock Road, 59763, Singapore 3- Poultry Cooperative Research Centre (CRC), Armidale NSW, Australia
  2. 2. Aims and objectives What is known?  Several studies have been conducted to assess the impact of this DDGS on broiler chicken performance.  Previous positive response to protease (Peek et al., 2009) for coccidial infection and xylanase (Choct et al., 1999) for suppression of C. perfringens in the intestine of broilers What is unknown?  Any possible health or immune modulatory effect of DDGS in poultry under a disease challenge (necrotic enteritis)?  What effect might the addition of protease and xylanase with inclusion of DDGS have under NE?
  3. 3. Methods A 2 × 2 × 2 factorial arrangement of treatments was used to investigate the effects of 3 factors: 1. Diets (0 and 20 % DDGS) 2. A blend of xylanase and protease supplementation (with or without), 3. NE challenge (challenged or unchallenged) and their interactions Wheat and soy based diets were formulated to be isoenergetic and isonitrogenous  Day 9 : Inoculation with Eimeria acervulina, E. maxima and E. bruneti.  Day 14, 15 and 16: oral gavages of C. perfringens Microbial profile in ileum and caeca before (and after challenge. Serum immunoglobulin by ELISA assays
  4. 4. Key results • Interaction of diet and challenge disease in infected birds fed diet containing DDGS after inoculations with C. perfringens led to poorer growth performance. Small intestine NE lesion score (d 17) FCR 14-21 d 1.2 a 2.5 a 1 a b c c Unchallenged 0.4 FI(g)/WG(g) Lesion score bcd 0.6 1 0.5 d d d d bcd cd Unchallenged Challenged Challenged d bc 1.5 0.2 0 b 2 0.8 0
  5. 5. Key results (Gut microflora) Caecal C. perfringens (d 17) 6 a a 5 Log Cfu/g digesta  DDGS inclusion interacted with challenge disease increasing caecal C. perfringens counts  A tendency for suppression of Lactobacilli in ileal content of birds fed diet containing DDGS.  No significant effect of xylanase and protease c c 4 d d d 3 d Unchallenged Challenged 2 1 0 Control Cntl+Enzyme DDGS DDGS+Enzyme
  6. 6. Serum immunoglobulin Notable interaction: • Serum IgA and IgM response was lower in the infected birds fed diets containing DDGS (d 21). Main effects:  Incorporation of DDGS to the diets increased (P < 0.01) the IgA and IgG titers at d 13 (before C. p), but decreased them at d 21(after C. p)(P < 0.05).  Birds fed diets containing DDGS also had lower (P < 0.05) IgM at d 35.  Enzyme treatment independently increased (P <0.01) the IgG titer at d 21.
  7. 7. Implications  High dietary inclusion of DDGS may increase susceptibility to necrotic enteritis in broilers  Increased C. perfringens proliferation may be associated with lower protein or amino acid digestibility of the diet containing DDGS (Barekatain et al. 2013 Anim Feed Sci Tech)  By a tendency, supplementation of protease and xylanase helped the infected birds fed DDGS to maintain feed intake and BW without significant effect on gut microflora or serum immunoglobulins
  8. 8. Visit us at: DSM Animal Nutrition and Health