Recombinant antibodies are antibody fragments generated by using recombinant antibody coding genes as a source and display technology, delivering high reproducibility, specificity and scalability. Unlike monoclonal antibodies (mAbs) which are produced using traditional hybridoma technologies, rAbs do not need hybridomas and animals in the production process if you only use synthetic genes.

1. Introduction of
Recombinant
antibody
www.creative-biolabs.com

2. Conventional Antiboby
Antibody; Immunoglobulins Paratope of antibodyPlasma cells

3. Antibody Structure
Be composed of:
• Two heavy chains
• Two light chains
Two structural region:
• Constant region
• Variable region

4. Five Isotypes of Antibody
Antibody Isotype Type of Heavy Chain Sub-isotypes Structure Function
IgA α IgA1, IgA2 Monomer Tetramer
Preventing pathogens invading into tissues
from epiderm
IgD δ N/A Monomer
Activating basophils and mast cells to produce
antimicrobial factors
IgE ε N/A Monomer
Interacting with allergens, facilitating histamine
releasing from mast cells and basophils,
preventing worms infection
IgG γ
IgG1, IgG2, IgG3,
IgG4
Monomer
The major antibody fighting against
pathogens
IgM μ N/A
Monomer Provide early elimination of pathogens before

5. Antibody Isotypes
• Predominant in secretions
• Monomeric form in blood
IgA
IgD
• Function is unclear
• Receptor on B cells
IgG
• 4 subclasses due to variation in γ
• serum concentration:
• IgG1>IgG2>IgG3>IgG4
IgE
• Bound to surface of
mast cells/basophils
• Most efficient at antigen-binding
• Monomeric IgM as mIgM on B cells
• Joined by J chain
IgM

6. Recombinant Antibody Production
B cell repertoire
Antibody gene
Gene Clone
Recombinant DNA
Phage display
Yeast display
Ribosome display
Recombinant
antibody
Fab fragment
ScFv
Single domain
antibody (SdAb)

7. Three Common Recombinant Antibody Fragment
VH, VL and linker
without ch1
Camelid antibody
Shark antibodySingle domain
antibody(sdAb)
scFv
Fab
Papain

8. Advantage & Disadvantage of Recombinant Antibody
Recombinant Antibody
Smaller size monovalency;
Ease of engineering & manufacture;
Improved tissue penetration;
Lack of potentially deleterious Fc effector
function.
Conventional Antibody
Lower antibody yield;
Highly training & experienced technician.

9. Recombinant Antibody
Provided
by Creative Biolabs
Products:
• scFv Fragment Antibodies
• Fab Fragment Antibodies
• Single Domain Antibodies
• Rabbit Monoclonal Antibodies
Services:
• Recombinant Antibody Construction
• Recombinant Antibody Purification & Expression

10. Address: 45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-871-5806 Fax: 1-631-614-7828
Email: info@creative-biolabs.com
Web: www.creative-biolabs.com
Contact Us for Recombinant Antibody