Successfully reported this slideshow.
We use your LinkedIn profile and activity data to personalize ads and to show you more relevant ads. You can change your ad preferences anytime.

Commonly used protein expression systems—pros & cons & applications

Commonly used protein expression systems—pros & cons & applications

  • Be the first to comment

  • Be the first to like this

Commonly used protein expression systems—pros & cons & applications

  1. 1. Page 1 Commonly Used Protein Expression Systems— Pros & Cons & Applications —Creative BioMart
  2. 2. Page 2 Protein expression, a kind of molecular biology technology referring to the use of model organisms such as bacteria, yeast, animal cells, or plant cells to express foreign gene protein, plays an important role in the field of genetic engineering. This post is to make a comparison about the advantages, disadvantages and applications of some commonly used protein expression systems, including Escherichia coli expression system, Pichia pastoris expression system, Saccharomyces cerevisiae expression system, Mammalian expression system, Baculovirus Cell expression System, stable cell line expression system, etc.
  3. 3. Page 3 1. Escherichia coli expression system Advantages High-level expression; Low cost; Simple culture conditions; Fast expression; Extensible; Easy to manipulate; Easy to form disulfide bonds. Disadvantages Easy to form inclusion bodies; Ineffective in vitro folding; Different codon systems; Few post-translational modifications; Endotoxin Applications Production of purified protein (structure, enzyme, drug discovery); Drug protein production.
  4. 4. Page 4 2. Pichia pastoris expression system Advantages High-level expression; Low cost; Simple culture conditions; Fast expression; Extensible; Suite for secretory protein or intracellular expression; Protein secretion is efficient and allows simple purification; Extensive post- translational modification; N-terminal glycosylation is superior to Saccharomyces cerevisiae; No endotoxin. Disadvantages Use of methanol as an inducer has a certain hazard; Glycosylation is still different from mammalian cells. Applications Production of purified protein (structure, enzyme, drug discovery)
  5. 5. Page 5 3. Saccharomyces cerevisiae expression system Advantages High-level expression; Low cost; Suitable for secretory protein or intracellular; Simple culture conditions; Extensible; Effective protein folding; No endotoxin. Disadvantages Secretory capacity may be lower than Pichia pastoris; Glycosylation is different from mammalian cells; Glycosylation N-terminal glycosyl chain structure has sensitization. Applications Production of purified protein (structure, enzyme, drug discovery)
  6. 6. Page 6 4. Mammalian cells expression system Advantages Higher-level expression; Moderate scalability; Suspension culture characteristics of the cells can be produced on a large scale; Effective protein folding; Suitable for secreting proteins; Full post-translational modification; No endotoxin. Disadvantages Expensive medium; Complex growth conditions Applications Production of purified protein (structure, enzyme, drug discovery); Drug protein production; Cell—based studies.
  7. 7. Page 7 5. Stable cell line expression system Advantages Protein production lines can be obtained Disadvantages Production is slow (takes several months), especially in the case of selective cloning, the ability to express in the passage of the process may decline odification; No endotoxin.
  8. 8. Page 8 6. Baculovirus-Insect Cell expression Systems Advantages High-level expression (especially intracellular protein); Faster growth rate; Effective cell folding; Moderate scalability; Extensive post- translational modification; Glycosylation is similar to mammalian cells; Relatively easy enzymatic de-glycosylation (beneficial for protein structure assays); No endotoxin. Disadvantages Expensive medium; Need a lot of virus; Secretion pathway of the pro- peptide is inefficient; Glycosylation is still different from mammalian cells; Virus infection can lead to cell lysis and potential expression of protein degradation. Applications Production of purified protein (structure, enzyme, drug discovery)
  9. 9. Page 9 Thanks for watching! Contact Creative BioMart Address: 45-1 Ramsey Road Shirley, NY 11967, USA Email: contact@creative-biomart.com Website: http://www.creativebiomart.net

    Be the first to comment

    Login to see the comments

Commonly used protein expression systems—pros & cons & applications

Views

Total views

1,802

On Slideshare

0

From embeds

0

Number of embeds

0

Actions

Downloads

43

Shares

0

Comments

0

Likes

0

×