Chelsea Technologies GroupChelsea Technologies GroupJustin Dunning, Sales ManagerChelsea Technologies Group       UviLux W...
Chelsea Technologies GroupOceanographic                            Water               Fresh water &     Exhaust gas     A...
Marine• Oceanography   - Deep ocean research   - Climate change monitoring   - Biodiversity   - Long term monitoring syste...
Marine – towed vehicle systems   UviLux Workshop, July 2012
Marine – acoustic products   UviLux Workshop, July 2012
Defence – submarine systems   UviLux Workshop, July 2012
Defence – ship systems   UviLux Workshop, July 2012
Defence – acoustic systems   UviLux Workshop, July 2012
CPNI – water protection• Contamination detection   - Bioterrorism   - Agricultural run-off   - Industrial   - Hydrocarbon ...
Environmental• Fresh water monitoring    - Ecological assessment    - Algal bloom detection    - Acoustic impact studies• ...
Maritime - FerryBox   UviLux Workshop, July 2012
Maritime - exhaust gas scrubbing monitors • Onboard systems allow use of existing   low sulphur fuels • Avoid expensive fu...
Maritime - ballast water monitoring • Prevent the transport of invasive species • IMO Ballast Water Convention 2004 • All ...
New fluorometers for the Water Industry • UniLux/TriLux    - Miniature single & multiwavelength fluorometers    - Pigment ...
UniLux single wavelength fluorometers• Referenced LED excitation    - High sensitivity & stability• Single window geometry...
TriLux multiwavelength fluorometers• 3-channel multi-wavelength fluorometer    - Same form factor as UniLux    - 3 excitat...
Algal fluorescence induction spectra             Ulva sp. (Sea lettuce)                                      Porphyridium ...
Fast Repetition Rate Fluorometry (FRRf)• Development from conventional chlorophyll fluorimetry• Specific tool to study phy...
FRRF applied to water toxicity monitoring• Toxins disrupt algal physiology and photosynthetic pathways• Use natural algal ...
UviLux – currently 4 configurations                             PAH          CDOM          Optical Brighteners       Trypt...
What is Tryptophan? • An essential amino acid in human diet • Main component of protein fluorescence • Associated with mic...
Fluorescence correlation to BOD• Strong relationship between fluorescence and BOD5 demonstrated• Applications:   - Surroga...
Tryptophan UviLux•   Currently the only commercial in situ Tryptophan fluorometer available•   Why measure fluorescence?  ...
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Water Quality Monitoring from Chelsea Technologies Group

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Chelsea Technologies Group (CTG) has nearly 50 years experience in the design and manufacture of a range of sensors and systems for the marine, environmental, defence, homeland security, industrial process control and life science markets. Our technology is being pioneered for a range of diverse applications.

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Water Quality Monitoring from Chelsea Technologies Group

  1. 1. Chelsea Technologies GroupChelsea Technologies GroupJustin Dunning, Sales ManagerChelsea Technologies Group UviLux Workshop, July 2012
  2. 2. Chelsea Technologies GroupOceanographic Water Fresh water & Exhaust gas Application of Naval Defenceresearch Quality/Treatment coastal monitoring sensor monitoring technologySensors & Tactical Ballast water Bio/chem Security Offshore pipelinesystems Oceanography monitoring Process control & leak detection monitoringAcoustic Storage Tank FerryBox Acoustic Target Hydrocarbontransducers Security Medical Simulation pollution Security diagnosticsFisheries monitoring Environmental Algal biofuel production UviLux Workshop, July 2012
  3. 3. Marine• Oceanography - Deep ocean research - Climate change monitoring - Biodiversity - Long term monitoring systems• Products - Sensors & systems - Towed vehicles - Acoustic transducers UviLux Workshop, July 2012
  4. 4. Marine – towed vehicle systems UviLux Workshop, July 2012
  5. 5. Marine – acoustic products UviLux Workshop, July 2012
  6. 6. Defence – submarine systems UviLux Workshop, July 2012
  7. 7. Defence – ship systems UviLux Workshop, July 2012
  8. 8. Defence – acoustic systems UviLux Workshop, July 2012
  9. 9. CPNI – water protection• Contamination detection - Bioterrorism - Agricultural run-off - Industrial - Hydrocarbon - Sewage / waste water• Fast Repetition Rate Fluorometry - FastGuard UviLux Workshop, July 2012
  10. 10. Environmental• Fresh water monitoring - Ecological assessment - Algal bloom detection - Acoustic impact studies• Coastal monitoring - Corals/seagrasses - Waste water outflow - Fisheries• Offshore pipeline leak detection• Hydrocarbon pollution monitoring - Legislation following BP Macondo incident UviLux Workshop, July 2012
  11. 11. Maritime - FerryBox UviLux Workshop, July 2012
  12. 12. Maritime - exhaust gas scrubbing monitors • Onboard systems allow use of existing low sulphur fuels • Avoid expensive fuels and engine modifications • Hamworthy Krystallon - New build Messina vessels - Retrofit APL vessels - New Solvang vessels • PAH, Turbidity & pH sensor suite UviLux Workshop, July 2012
  13. 13. Maritime - ballast water monitoring • Prevent the transport of invasive species • IMO Ballast Water Convention 2004 • All new builds from 2012 • Vessels built before 2012 must be fitted by 2017 • Estimated 62,000 MWTS required UviLux Workshop, July 2012
  14. 14. New fluorometers for the Water Industry • UniLux/TriLux - Miniature single & multiwavelength fluorometers - Pigment and dye tracing - Algal group studies & bloom detection • Fast Repetition Rate Fluorometry (FRRf) - Active fluorescence - Contamination detection - Ecological monitoring • UviLux - New range of low cost UV fluorometers - PAH, CDOM, Optical Brighteners & Tryptophan UviLux Workshop, July 2012
  15. 15. UniLux single wavelength fluorometers• Referenced LED excitation - High sensitivity & stability• Single window geometry - Suitable for wipers• High quality optical filtration - Low background - Low turbidity breakthrough• Microprocessor control - Digital output in calibrated engineering units - User control of instrument parameters• Parameters - Chlorophyll-a, phycobiliproteins, fluorescein, rhodamine-wt & turbidity UviLux Workshop, July 2012
  16. 16. TriLux multiwavelength fluorometers• 3-channel multi-wavelength fluorometer - Same form factor as UniLux - 3 excitation wavelengths - Common detection at 685nm• Application - Contribution to chlorophyll fluorescence from light harvesting pigments - Algal class studies - Bloom detection• 3 configurations available - Chlorophyll-a, phycocyanin, turbidity (freshwater) - Chlorophyll-a, phycoerytherin, phycocyanin (coastal) - Chlorophyll-a, phycoerythrin, turbidity (seawater) UviLux Workshop, July 2012
  17. 17. Algal fluorescence induction spectra Ulva sp. (Sea lettuce) Porphyridium cruentum Green algae (Chlorophyll b) Marine, unicellular red algae Wavelength (nm) Wavelength (nm) Chaetoceros gracilis Chroomonas sp. (Cryptophyte) Marine diatom (Chlorophyll c) Marine cyanobacterium – more chlorophyll than most cyanobacteria + phycocyanin only Dashed line = 685 nm Heavy line = 730 nm Light line = O2 evolution Wavelength (nm) Wavelength (nm) UviLux Workshop, July 2012
  18. 18. Fast Repetition Rate Fluorometry (FRRf)• Development from conventional chlorophyll fluorimetry• Specific tool to study phytoplankton physiology• Used for open ocean research - Photosynthesis efficiency, biomass, primary photosynthesis/productivity - Carbon dioxide fixation, climate modelling - Bloom detection• Applications: - Homeland security - Industrial contamination detection - Environmental monitoring - Bioreactor process monitoring UviLux Workshop, July 2012
  19. 19. FRRF applied to water toxicity monitoring• Toxins disrupt algal physiology and photosynthetic pathways• Use natural algal population as transducer - Provides more specific information than conventional fluorimetry - Broad spectrum detection capability• Time dependent measurement - Dimensionless parameters - Less susceptible to natural variations - Multi-parameter sensor• Potential applications - Water intake protection - Bloom detection - Ecological monitoring UviLux Workshop, July 2012
  20. 20. UviLux – currently 4 configurations PAH CDOM Optical Brighteners Tryptophan Excitation 255nm 255nm 350nm 280nm Emission 360nm 450nm 450nm 360nm Sensitivity 0.005µg/L 0.002µg/L 0.005µg/L 0.02µg/L (carbazole) (perylene) (stilbene derivatives) (Tryptophan) Range 0.005 – 200µg/L 0.002 – 15µg/L 0.005 – 200µg/L 0.02 – 800µg/L Depth Rating 600m 600m 600m 600m UviLux Workshop, July 2012
  21. 21. What is Tryptophan? • An essential amino acid in human diet • Main component of protein fluorescence • Associated with microbial activity - Sewage & faecal contamination of waste waters, e.g. agricultural runoff • Fluorescence correlates with BOD measurements UviLux Workshop, July 2012
  22. 22. Fluorescence correlation to BOD• Strong relationship between fluorescence and BOD5 demonstrated• Applications: - Surrogate for Biological Oxygen Demand (BOD) measurement - Effluent contamination monitoring - Combined Sewage Overflow (CSO) and Fish Kill investigations - General pollution event investigations - Foul & surface sewer misconnection detection - Water catchment surveys ‘Should consider fluorescence spectroscopy as a more accurate, independent and flexible indicator of bioavailability than BOD5’ Science of the Total Environment 391 (2008) 149 – 158 UviLux Workshop, July 2012
  23. 23. Tryptophan UviLux• Currently the only commercial in situ Tryptophan fluorometer available• Why measure fluorescence? - Sensitivity - typically x1000 absorbance techniques - Specificity – not everything fluoresces• Why measure in situ? - Spot sampling - Avoids sample transport for laboratory testing - Over sampling improves data quality - High spatial and temporal resolution• Features - Robust stable performance - Combines high sensitivity with wide dynamic range - Digital & analogue outputs - Wireless connectivity for field use - Inline options available UviLux Workshop, July 2012

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