Seminario BM

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Seminario BM

  1. 1. The use of caspase inhibitors in pulsed-field gelelectrophoresis may improve the estimation ofradiation-induced DNA repair and apoptosis<br />Josep Balart, Gemma Pueyo, Lara I de Llobet, Marta Baro, Xavi Sole, Susanna Marin, Oriol Casanovas,Ricard Mesia, Gabriel Capella.<br />SIMON RESTREPO<br />CAMILO RESTREPO<br />
  2. 2. KEY WORDS <br />PFGE Pulsed Field Gel Electrophoresis.<br />CASPASES Proteins that are one of the main executors of the apoptotic process and the pro-inflammatory process too.<br />DNA REPARATION Processes by which a cell identifies and corrects damage to the DNA molecules that encode its genome. Mechanisms<br />APOPTOSIS Process of programmed cell death that may occur in multicellular organisms.<br />
  3. 3. INTRODUCTION<br />DNA repair in several factors which can be determined through PFGE, as are the activity mediated by caspases called programmed cell death, apoptosis. <br />Then the objective of PFGE is determine the magnitude of radiation induced by these processes and determine that the release of DNA from cells corresponds to the intensity of the DNA fragmentation<br />
  4. 4. In the PFGE technique, cells are encapsulated in agarose to form cell-plugs, thus preventing physic damage of the cells while facilitating their manipulation and placement into agarose gels where electrophoresis will take place.<br />
  5. 5. GENERAL OBJECTIVE<br />Determine the radiation generated by PFGE of DNA fragmentation and apoptosis during repair.<br />
  6. 6. MATERIALES Y METODOS<br />LINEAS CELULARES<br />Células tumorales A431 Carcinoma Escamoso.<br />Células NP18 Carcinoma Pancreático.<br />
  7. 7. MUESTRA<br />Ratones de 6 a 8 semanas de sexo masculino.<br />
  8. 8. PROCEDIMIENTO<br /> A cada ratón se le inyecto de forma SC NP18 en su flanco para generar el tumor.<br />10mm Extracción/extirpación 90min incubación DMEM 30min Tripsina a 37° en agitación<br />
  9. 9. ELCTROFORESIS<br />Separación de partículas por peso y por cargas<br />
  10. 10. PFGE<br />Herramienta para examinar el ADN cromosómico que es capaz de separar fragmentos de 10 kpb hasta 10.000 kpb. <br />
  11. 11. FLUORESCENCIA<br />Es la propiedad de una sustancia de emitir luz cuando es expuesta a radiaciones<br />
  12. 12. INMUNOFLUORESCENCIA<br />La inmunofluorescencia se utiliza esencialmente en la detección de autoanticuerpos y anticuerpos contra antígenos de superficie de células y tejidos.<br />
  13. 13. DIRECTA<br />
  14. 14. INDIRECTA<br />
  15. 15. Resultados<br />
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  21. 21. Discussion<br />
  22. 22.
  23. 23. CONCLUSIONS<br />New techniques may improve better and reliable results<br />There should be more invest on this kind of studies<br />This study shed a light to develop better treatments.<br />Radiationdoesnot cure, itsjust a waytopreventcancer spreads.<br />
  24. 24. Mapas Conceptuales<br />
  25. 25. Simón Restrepo<br />
  26. 26. CAMILO RESTREPO RODRIGUEZ<br />
  27. 27. Thanks<br />

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