Process of isolating
chromosomes of White mice

By: Heydee A. Santos
Chromosome Preparation:
 Chromosome of the bone marrow cells and
testis were prepared afte one hour, the
treatment into 0...
Each Mouse was Weighed Prior to
Colchicine Treated
The Mice were Pre-Treated with
0.25ml Colchicine one hour
before
Chromosome Preparation:
The testis was pulled just above the penile organ
through an incision, again these was placed in a...
Slide Plating:
 Dried clean microscope slides (25x 75 mm)
previously soaked in a 1:1 ratio of Ethanol – Acetic
acid solut...
Suspension was Topped o a PreCleaned Slide under an Infra – red
Light.
Analysing chromosome
Normal chromosome of testis
Chromosomal Aberration on
testis
Chromosomal Break at Control – Irridiated
Mouse Bone Marrow Cells at Mitotic Metaphase
Sticky Chromosome
Reason of using white mice as a
biological indicator

The mice were used as they are good
biological indicator organism fo...
Thank You!
Upcoming SlideShare
Loading in …5
×

Isolating Chromosomes

591 views

Published on

0 Comments
0 Likes
Statistics
Notes
  • Be the first to comment

  • Be the first to like this

No Downloads
Views
Total views
591
On SlideShare
0
From Embeds
0
Number of Embeds
1
Actions
Shares
0
Downloads
11
Comments
0
Likes
0
Embeds 0
No embeds

No notes for slide

Isolating Chromosomes

  1. 1. Process of isolating chromosomes of White mice By: Heydee A. Santos
  2. 2. Chromosome Preparation:  Chromosome of the bone marrow cells and testis were prepared afte one hour, the treatment into 0.025% colchicine (Fig. 6) at 0.25%ml per kilogram body weight. (Fig.10) the mice were sacrificed by cervical dislocation. The femur yields the bone marrow cells which were pumped out into the isotonic solution (2.2% sodium Citrate) through the sucking and pumping motion of the syringe with a fine needle.
  3. 3. Each Mouse was Weighed Prior to Colchicine Treated
  4. 4. The Mice were Pre-Treated with 0.25ml Colchicine one hour before
  5. 5. Chromosome Preparation: The testis was pulled just above the penile organ through an incision, again these was placed in an isotonic solution 2.2% (Sodium Citrate) for teasing to a fine suspension though a syringe. The bone marrow cells and testis suspension were transferred to a dryer tube. Spinning for five (5) minutes (Fig. 8), the supernatant was removed for cell breaking and stocking in a hypotonic solution (1.0% sodium citrate) for twelve (12) minutes. Fixation follows, for the testis cells suspension, three (3) fixation processed are needed. Flicking of the tube is also needed for the recovering of metaphase. Four (4) fixations are needed for the bone marrow cells suspension.
  6. 6. Slide Plating:  Dried clean microscope slides (25x 75 mm) previously soaked in a 1:1 ratio of Ethanol – Acetic acid solution, using a fine – tipped pasteur pipettes, a suspension was topped on a pre cleaned slide and dried by blowing the suspension under an infra – red light. (Fig. 9). Slide was stained with temporary stain Toluidine – o, and covered with cover slips, and observed with Zeiss standard 18 microscope camera attachment using 25 – 40x objectives and a 10x / 18 oculars with plerapo 100 / 1.3 oil immersion objective, green filter and colpan 21 high copy film, it is an important Panchromatic fild packed by Comlumbia
  7. 7. Suspension was Topped o a PreCleaned Slide under an Infra – red Light.
  8. 8. Analysing chromosome
  9. 9. Normal chromosome of testis
  10. 10. Chromosomal Aberration on testis
  11. 11. Chromosomal Break at Control – Irridiated Mouse Bone Marrow Cells at Mitotic Metaphase
  12. 12. Sticky Chromosome
  13. 13. Reason of using white mice as a biological indicator The mice were used as they are good biological indicator organism for the following reason: (1) readily available (2) low cost (3) ease of handling (4) good chromosome condition and (5) have good correlation with other test organism.
  14. 14. Thank You!

×