Citrex 0610 I En Revised Spa 1 Citrex Mic Mbc

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Citrex 0610 I En Revised Spa 1 Citrex Mic Mbc

  1. 1. IN VITRO EVALUATION THE CITREX® MOLECULE INHIBITORY/CIDAL ACTIVITY AGAINST DIFFERENT ORGANISMS HAROLD EDUARDO DURANGO G. Medical Mycology Bacteriologist Professor of Microbiology and Parasitology. Medicine Faculty, U. of A. JOSE IGNACIO BARGUIL P. Food Microbiology Bacteriologist Food protection technical consultant, Consultant for Banco Interamericano de Desarrollo, BID. Technical Director, Continua Consultores. Pediatric Infectology Research Laboratory. H.U.S.V.P
  2. 2. Microbiological assays using CITREX ® liquid <ul><li>Minimal inhibitory concentrations for several organisms relevant for both the food industry and human health were determined. </li></ul>
  3. 3. Organisms challenged <ul><li>Pseudomonas aeruginosa ATCC 27853 </li></ul><ul><li>Escherichia coli ATCC 25922 </li></ul><ul><li>Salmonella typhimurium ATCC 14028 </li></ul><ul><li>Vibrio cholerae ATCC 14035 </li></ul><ul><li>Staphylococcus aureus ATCC 29213 </li></ul><ul><li>Enterococcus faecalis ATCC 29212 </li></ul><ul><li>Candida albicans ATCC 14053 </li></ul><ul><li>Aspergillus flavus Environmental isolate </li></ul>
  4. 4. CITREX ® concentrations tested 6.25 Tube 9 12.5 Tube 8 25 Tube 7 50 Tube 6 100 Tube 5 200 Tube 4 400 Tube 3 800 Tube 2 1000 Tube 1 Citrex® Concentration (ppm) Tube number
  5. 5. Process: <ul><li>Organisms were cultured in BHI (brain/heart infusion) medium, 24 h @ 37°C, in order to have activated strains. </li></ul><ul><li>Organism suspension equivalent to McFarland´s pattern of 10 6 CFU/ml. </li></ul><ul><li>10-tube series per organism corresponding different CITREX ® concentrations . </li></ul>
  6. 6. Process: <ul><li>Assay procedure: To 1 ml sterile BHI broth add 1 ml of the desired CITREX solution per tube and 1 ml standardized 10 6 CFU/ml organism suspension, in agreement with McFarland’s turbidity scale. </li></ul>
  7. 7. Controls for test validation <ul><li>Control tube 1: Organism livability control: Add 1 ml sterile BHI broth and 1 ml organism suspension, in each case. </li></ul><ul><li>Control tube 2: CITREX solution sterility control: Add 1 ml sterile BHI broth and 1 ml CITREX preparation. Discard 1 ml from last dilution tube. </li></ul><ul><li>Control tube 3: BHI broth sterility control: Add 2 ml BHI broth per tube. </li></ul>
  8. 8. CITREX ® Minimum Inhibitory Concentrations Test 1: + + + - - - - - - A. flavus + + - - - - - - - C. albicans + + - - - - - - - E. faecalis + - - - - - - - - S. aureus + + + + + - - - - V. cholerae + + + + + - - - - S. typhimurium + + + + + - - - - E. coli + + + + + + + - - P. aeruginosa CITREX 6.25 ppm CITREX 12.5 ppm CITREX 25 ppm CITREX 50 ppm CITREX 100 ppm CITREX 200 ppm CITREX 400 ppm CITREX 800 ppm CITREX 1000 ppm Microorganism
  9. 9. CITREX ® Minimum Inhibitory Concentrations Test 2: + + + - - - - - - A. flavus + - - - - - - - - C. albicans + - - - - - - - - E. faecalis + - - - - - - - - S. aureus + + + + + - - - - V. cholerae + + + + + - - - - S. typhimurium + + + + + - - - - E. coli + + + + + + + - - P. aeruginosa CITREX 6.25 ppm CITREX 12.5 ppm CITREX 25 ppm CITREX 50 ppm CITREX 100 ppm CITREX 200 ppm CITREX 400 ppm CITREX 800 ppm CITREX 1000 ppm Microorganism
  10. 10. Test 3: CITREX ® Minimum Inhibitory Concentrations + + + - - - - - - A. flavus + + - - - - - - - C. albicans + - - - - - - - - E. faecalis + - - - - - - - - S. aureus + + + + + - - - - V. cholerae + + + + + - - - - S. typhimurium + + + + + - - - - E. coli + + + + + + + - - P. aeruginosa CITREX 6.25 ppm CITREX 12.5 ppm CITREX 25 ppm CITREX 50 ppm CITREX 100 ppm CITREX 200 ppm CITREX 400 ppm CITREX 800 ppm CITREX 1000 ppm Microorganism
  11. 11. Result summary from all 3 tests * Corresponds to MIC in 2 of 3 tests 50 A. flavus 12.5 – 25* C. albicans 12.5 – 25* E. faecalis 12.5 S. aureus 200 V. cholerae 200 S. typhimurium 200 E. coli 800 P. aeruginosa Minimum Inhibitory Concentration (ppm CITREX ®) Microorganism
  12. 12. MINIMUM BACTERICIDAL CONCENTRATION <ul><li>MBC’s are more useful to follow up drug’s microbicidal activity in patients under specific antibiotic therapy. Even though it can be useful if taken into account at the time of choosing a product for the control of specific processes. </li></ul><ul><li>MBC is determined by culture in a gel medium from the 4 tubes with the highest test drug, from the tube representing the Minimum Inhibitory Concentration. </li></ul>
  13. 13. CITREX ® Liquid Minimal Bactericidal Concentration 200 A. flavus 50 C. albicans 50 E. faecalis 25 S. aureus 400 V. cholerae 400 S. typhimurium 800 E. coli 1000 P. aeruginosa Minimum Bactericidal Concentration (ppm) Microorganism
  14. 14. RESULT DISCUSSION <ul><li>Citrex ® molecule performance was consistent with expected results, within margins established for this class of disinfectants. </li></ul><ul><li>The product was evaluated against ATCC strains, which are clinically used in the quality control of sensitivity tests and in evaluating the activity of antimicrobials. </li></ul>
  15. 15. <ul><li>Variation in one concentration in the case of Enterococcus faecalis and Candida albicans , is probably due to the inoculum effect (a drift in the standardization of bacterial suspension, when compared with McFarland’s turbidity pattern). </li></ul><ul><li>Therefore, result summary table shows a MIC range for these organisms in bold (*); i.e. the result from 2 of the 3 assays performed . </li></ul>RESULT DISCUSSION

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