Precision phenotyping for improvingdrought stress tolerant maize in ThailandSutatsane VongsupathaiSuriphat     ThaitadTats...
Problem• In Thailand, drought was reported as a  priority constrain for maize production  in many agro-ecological zones an...
Objectives • To compare the   performance of   hybrids maize and   to understand the   association of yield   with morphol...
Materials and methodsMaterials :• The set of hybrids maize from CIMMYT    148 + 2 local check hybrids    Local checks :NS2...
RH%(max)                                Rain(mm.)           Max.temp (oC)                                                 ...
Water management:    Well watered (ww)      : weekly irrigation about 30 mm./time   Water stress (Flowering stage drought)...
Data Collections Agronomic traits Secondary traits                        7
Agronomic traits                                 Root and stalk lodging                                                   ...
Secondary traits1) anthesis-silking interval (days)2) leaf rolling : - 2,3 weeks after stop irrigation   (Score 1-5)    - ...
4) Normalized Difference Vegetative Index (NDVI)  Green Seeker: NDVI values can be used to predict  green biomass    Veg...
5) Stomata conductance (mmol/H2O m-2s-1)  5) Stomata conductance and Leaf temperature Leaf porometer : Vegetative stage ...
6) Leaf temperature (Co) Infrared thermometer: Vegetative stage : 4-5 weeks after planting and again 2 weeks later. Rep...
7) Chlorophyll content SPAD meter : Flowering stage : 1 time. Grain filling : every week during grain filling for 5 wee...
14
Table 1 Mean grain yield (ton/ha) of top ten hybrids maize                       under water stress condition at NSFCRC in...
Table 2 Phenotypic correlation coefficient (r) between grain        yield and importance agronomic traits, 2010D.         ...
Table 3 Phenotypic correlation coefficient (r) between        grain yield and NDVI, 2010D.            Green Seeker        ...
Table 4 Phenotypic correlation coefficient (r) between        grain yield and stomata conductance, 2010D.        Leaf poro...
Table 5 Phenotypic correlation coefficient (r) between        grain yield and leaf temperature (oC), 2010D.Infrared thermo...
Table 6 Phenotypic correlation coefficient (r) between        grain yield and chlorophyll content, 2010D.          SPAD me...
1 weeks after stop irrigation                                21
2 weeks after stop irrigationLa Posta Seq C7-F18-3-2-1-1-B-B-B-B-B / CML-   (A.T.Z.T.R.L.BA90 5-3-3P-1P-4P-2P-1-1-1-B x G9...
3 weeks after stop irrigation   4 weeks after stop irrigation                                                             ...
WELL WATERED                             WATER STRESS       CML-442/CML-444 (Check 3 late maturity)                  NS 3 ...
WELL WATERED                                WATER STRESS    La Posta Seq C7-F78-2-1-1-1-B-B-B-B / CML-312 SR              ...
Conclusions• Selection for drought tolerance by  increase EPP, HKW, CHE, NDVI and  stomata conductance were linked to  hig...
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S1.3.Precision phenotyping for improving drought stress tolerant maize in Thailand

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Presentacion de 11th Asian Maize Conference which took place in Beijing, China from November 7 – 11, 2011.

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S1.3.Precision phenotyping for improving drought stress tolerant maize in Thailand

  1. 1. Precision phenotyping for improvingdrought stress tolerant maize in ThailandSutatsane VongsupathaiSuriphat ThaitadTatsanee BudthongPichet Grudloyma Nakhon Sawan Field Crops Research Center (NSFCRC) Tak Fa, Nakhon Sawan, THAILAND 1
  2. 2. Problem• In Thailand, drought was reported as a priority constrain for maize production in many agro-ecological zones and it is observed almost every year.• Maize is usually affected to drought stress at difference stage. 2
  3. 3. Objectives • To compare the performance of hybrids maize and to understand the association of yield with morphological and physiological traits under water stress condition 3
  4. 4. Materials and methodsMaterials :• The set of hybrids maize from CIMMYT 148 + 2 local check hybrids Local checks :NS2 and NS3Design :• 10x15 Alpha lattice design with 2 replications• 2 rows/ plot of 5.0 m. long• 66,000 plants ha-1Planting period :• during dry season in 2009, NSFCRC. 4
  5. 5. RH%(max) Rain(mm.) Max.temp (oC) Flowering Min.temp (oC)Planting date 3 Dec 2009 Stop irrigatedTemperature (oC) rain fall (mm.) and RH distribution at NSFCRC, from Dec 2009 to Feb 2010 5
  6. 6. Water management: Well watered (ww) : weekly irrigation about 30 mm./time Water stress (Flowering stage drought) (WS) : The irrigation was stopped from 9th leaf stage to two weeks after mid flowering (15 Jan-15 Feb. 2010) WW WSWell watered Flowering drought 6
  7. 7. Data Collections Agronomic traits Secondary traits 7
  8. 8. Agronomic traits Root and stalk lodging Moisture content Field weight Anthesis date Hundred kernel weight Silking date Plant and ear height 8
  9. 9. Secondary traits1) anthesis-silking interval (days)2) leaf rolling : - 2,3 weeks after stop irrigation (Score 1-5) - before re-irrigation3) Senescence: - 3 weeks after stop irrigation (Score 1-10) - before re-irrigation - 30 days after silking 9
  10. 10. 4) Normalized Difference Vegetative Index (NDVI)  Green Seeker: NDVI values can be used to predict green biomass  Vegetative stage : Weekly measurements from 3 leaves until 8 leaves stage.  Grain filling stage : weekly from two weeks after male flowering till one week prior maturity 10
  11. 11. 5) Stomata conductance (mmol/H2O m-2s-1) 5) Stomata conductance and Leaf temperature Leaf porometer : Vegetative stage : 4-5 weeks after planting and again in 2 weeks later. Reproductive stage : 2 day after stopped irrigation and then every weekly until re-irrigation. Measurements : - 3 plants/ plot. - one leaf per plant - at the 4th leaf below flag leaf. -10-15 h. sunydays 11
  12. 12. 6) Leaf temperature (Co) Infrared thermometer: Vegetative stage : 4-5 weeks after planting and again 2 weeks later. Reproductive stage : 2 day after stopped irrigation and then every weekly until re-irrigation. Measurements : - 3 plants/ plot. - one leaf per plant - at the 4th leaf below flag leaf. -10-15 h. sunny days 12
  13. 13. 7) Chlorophyll content SPAD meter : Flowering stage : 1 time. Grain filling : every week during grain filling for 5 weeks. Measurements : - 6 plants/ plot. - one leaf per plant. - at the 4th leaf below flag leaf. 13
  14. 14. 14
  15. 15. Table 1 Mean grain yield (ton/ha) of top ten hybrids maize under water stress condition at NSFCRC in 2010D.No. Pedigree WS WW ASI (days)1 NS 3 (Local check 2) 5.09 8.26 22 CLQ-RCYQ54=(CML176 x CL-G2501)-B-3-1-B-B-B-B-B / CML-312 SR 5.00 6.79 23 DTPWC9-F67-2-2-1-B-B-B-B-B / CML-312 SR 4.84 7.59 34 [P591c4 1y2 GEN F23-1-1-1-B-B-B x CML486]-1-1-B-B / CML-312 SR 4.77 6.62 35 CML-332-B-B / CML-312 SR 4.73 7.54 46 CL-04935=(PR8549xP23C2)-5-1-3-B*14-B-B-B-B-B-B / CML-312 SR 4.41 5.68 47 DTPWC9-F24-4-3-1-B-B-B-B-B / CML-312 SR 4.32 7.00 18 La Posta Seq C7-F78-2-1-1-1-B-B-B-B / CML-312 SR 4.30 8.27 59 S87P69Q(SIYF) 109-1-1-4-B / CML-312 SR 4.21 6.33 210 XZY 460-1 / CML-312 SR 4.21 7.63 311 CML-442/CML-444 (Check 3 late maturity) 2.49 8.89 10 Mean 2.59 6.48 5 LSD(0.05) 1.55 1.14 2
  16. 16. Table 2 Phenotypic correlation coefficient (r) between grain yield and importance agronomic traits, 2010D. Grain Yield (t/ha) Traits WS Anthesis silking interval (ASI) -0.2745* Leaf senescence -0.4158* Leaf rolling -0.5199* Ear per plant 0.2317* Hundred kernel weight 0.2729* Moisture content 0.3746* 16
  17. 17. Table 3 Phenotypic correlation coefficient (r) between grain yield and NDVI, 2010D. Green Seeker Grain Yield (t/ha) (Day after anthesis) WS -41 0.0687ns -34 0.1291ns -25 0.2098* -18 0.1444ns 15 0.3785* 21 0.2398* 28 0.2800* 35 0.2302* Flowering drought period 17
  18. 18. Table 4 Phenotypic correlation coefficient (r) between grain yield and stomata conductance, 2010D. Leaf porometer Grain Yield (t/ha) (Day after anthesis) WS -23 0.1676* -9 0.1172ns 7 0.3572* 15 0.2087* 21 0.0805ns 35 0.0578ns Flowering drought period 18
  19. 19. Table 5 Phenotypic correlation coefficient (r) between grain yield and leaf temperature (oC), 2010D.Infrared thermometer Grain Yield (t/ha) (Day after anthesis) T1S2 (WS) -23 -0.1127ns -9 -0.1245ns 7 -0.3635* 15 -0.1435ns 21 -0.0393ns 35 -0.0637ns Flowering drought period 19
  20. 20. Table 6 Phenotypic correlation coefficient (r) between grain yield and chlorophyll content, 2010D. SPAD meter Grain Yield (t/ha) (Day after anthesis) WS 4 0.3628* 11 0.4911* 19 0.5531* 26 0.4468* 35 0.2170* 42 0.1739* Flowering drought period 20
  21. 21. 1 weeks after stop irrigation 21
  22. 22. 2 weeks after stop irrigationLa Posta Seq C7-F18-3-2-1-1-B-B-B-B-B / CML- (A.T.Z.T.R.L.BA90 5-3-3P-1P-4P-2P-1-1-1-B x G9B C0312 SR R.L.23-1P-2P-3-2P-3-2P-1P-B-B-B)-B-16TL-3-1-4-B-B- B / CML-312 SR 22
  23. 23. 3 weeks after stop irrigation 4 weeks after stop irrigation 23
  24. 24. WELL WATERED WATER STRESS CML-442/CML-444 (Check 3 late maturity) NS 3 (Local check 2) 24
  25. 25. WELL WATERED WATER STRESS La Posta Seq C7-F78-2-1-1-1-B-B-B-B / CML-312 SR XZY 460-1 / CML-312 SR 25
  26. 26. Conclusions• Selection for drought tolerance by increase EPP, HKW, CHE, NDVI and stomata conductance were linked to higher yield drought stress.• Reduction ASI, LRO, Senescence and leaf temperature suggesting that were increasing higher yield 26
  27. 27. Thank you

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