Successfully reported this slideshow.
We use your LinkedIn profile and activity data to personalize ads and to show you more relevant ads. You can change your ad preferences anytime.

High-resolution phenotyping Water flow dynamics Chloroplast movement

2,561 views

Published on

Remote sensing – Beyond images
Mexico 14-15 December 2013

The workshop was organized by CIMMYT Global Conservation Agriculture Program (GCAP) and funded by the Bill & Melinda Gates Foundation (BMGF), the Mexican Secretariat of Agriculture, Livestock, Rural Development, Fisheries and Food (SAGARPA), the International Maize and Wheat Improvement Center (CIMMYT), CGIAR Research Program on Maize, the Cereal System Initiative for South Asia (CSISA) and the Sustainable Modernization of the Traditional Agriculture (MasAgro)

Published in: Education, Technology, Business
  • Be the first to comment

  • Be the first to like this

High-resolution phenotyping Water flow dynamics Chloroplast movement

  1. 1. High-resolution phenotyping Water flow dynamics Chloroplast movement Hae Koo Kim, Joonghyuk Park, Jeong Eun Ryu, Sungsook Ahn, Ildoo Hwang and Sang Joon Lee
  2. 2. Plants in Environmental & Energy Sciences  Diversity in vascular system: Model plants  Central Role in the Water Cycle on Earth (conifers): water transport over 100m -Gymnosperms  Green revolution yet to come -Angiosperms (monocots & dicots): few cm to tens of m for smallholder farmers?  Importance of the Plants  Agriculture: up to 70% of the Water & mineral transport : Xylem water use  “Blue revolution” in Agriculture: Drought stress in water-limited environments - Drop by Drop - Gene by Gene Pennisi (Science, 2008)  Water transport in plants Cohesion-Tension Theory, Böhm, 1893; Dixon and Joly, 1895) Scholander et al. (1965) Science 148 Johnson & Dixon (1965) Nature 208 Scholander et al.,1965 Balling & Zimmermann, 1990 Pockman et al. (1995) Nature 378 Holbrook et al. (1995) Science 270
  3. 3. Visualization of Water flow at different level on Earth 6,650 km
  4. 4. Synchrotron facilities PAL, Pohang PF, Tsukuba SSRF, Shanghai
  5. 5. Visualization of water flow dynamics using synchrotron X-ray imaging Synchrotron X-ray imaging -Absorption and phase-contrast based imaging -High temporal (ms) and spatial resolution (μm) -Ideal to visualize xylem vessels of monocots -Real-time dynamics of refilling process Kim & Lee, 2010 New Phytologist
  6. 6. Phenotyping Xylem Structure/Function relationships Sap flow dynamics in xylem vessels Xylem Safety Efficiency Structure Fibers Vascular bundle [WATER TRANSPORT] Efficiency vs. Safety trade-off in water transport Function High resistance Modularity Support Repair Parenchyma Vessels Storage Transport Low resistance Connectivity Phloem FUNCTION of Xylem: Efficiency & Safety of water transport STRUCTURAL characteristics of XYLEM  Sap flow dynamics
  7. 7. Efficiency and Safety trade-offs in water conduits Number of pits 16-20 11-15 6-10 1-5 0 Cluster 1 Cluster 2-2 Cluster 2-1 U R 8 2 P, Q R 4 Pc P Cluster 3 8 Metaxylem vessel number and lumen area • Diameter A:B:C=4:2 :1 • Vessel area A : B : C = 16 : 4 : 1 • Flow rates A : B : C = 256 : 16 : 1 Cluster Number (ea) AVG (µm2) MAX (µm2) MIN (µm2) 1 73 226.7 (±102.3) 496.7 66.2 2 83 201.8 (±87.4) 489.6 76.0 3 126 108.7 (±37.5) 318.3 52.9 7
  8. 8. Visualization of internal structure of plants and flow dynamics Kim and Lee., 2010 New Phytologist Ahn et al., 2010 ACS Nano
  9. 9. -Long distance water transport (efficiency) -Short distance, higher resistance, local water distribution (safety)
  10. 10. Synchrotron X-ray CT: Vascular bundle organization in maize leaf
  11. 11. 3D organization of Vascular bundles in maize leaf
  12. 12. Xylem anatomical characteristics • Safety: Regulation of -Axial flow dynamics by perforation plates -Radial flow dynamics by pit membranes & network connexion Basis of embolism, cavitation mechanism Environmental effects: transpiration, osm ostic stress • Safety feature of perforation plates, lateral pits: air bubbles appearance/re moval, direct radial inflow of water  Network • Efficiency: -Effect of enhanced transpiration -Long distance water transport system In planta sap flow tracking Protoxylem (PX) Metaxylem (MX) •Use of various type of particles to investigate xylem cell wall properties (interactions of AuNPs with cell walls)
  13. 13. Flow dynamics in dicot plants (Arabidopsis) of dicot xylem structure Arabidopsis as model Experimental method: -Synchrotron X-ray CT [Xylem network 3D organization] -Hydrophilic AuNP solution [Indicator of sap flow rate] AuNP-OH Xylem SCW HO CH2 CH2 CH2 CH2 OH Difference of xylem vessel activity monitored by AuNP staining profile 13
  14. 14. Pattern of xylem vessel activity in normal condition Normal uptake profile (transpiration driven) Pattern of xylem vessel activity generated by external pressure Artificial uptake profile (external pressure driven)
  15. 15. Visualization of water transport pathways Hydrophilic gold-nanoparticles (AuNP) : tracers of axial & radial water flow pathways c Interestingly, all metaxylem vessels do not show the same level of activity for water transport 17
  16. 16. Regulation of water transport along plant height Differences in vascular bundle organization and pressure gradient directly impact AuNPs staining profile along the height of the inflorescence stem 18
  17. 17. Water flow pathway in the whole plant scale Efficient and safe water flow may coexist with one system as division into long-distance and local distribution 19
  18. 18. Visualization of chloroplast movement using two-photon microscopy Two-photon microscopy Tube lens Scan lens Ti:Sapphire laser Dichroic mirror DM1 Tube Scan lens lens Dichroic mirror DM2 Galvanometer scanner PMT Piezo stage Filter Objective lens Computer Translation stage
  19. 19. Monitoring of chloroplast movement depending on light S S S M BS S S S
  20. 20. Summary Place of plants in Environmental & Energy Sciences Importance of understanding Plant-Water relation Synchrotron X-ray imaging method is a powerful tool to study plant vascular structure and sap flow dynamics -Monocot and dicot plants 3D xylem structure (“Wiring diagram” of plant microfluidic system) -Demand-driven flow system -Efficiency and safety of water transport Perspectives of High-resolution phenotyping -Nano-CT of xylem -Optical Coherance tomography -Two-photon microscopy (photosynthesis)
  21. 21. Acknowledgements Advanced Biomass R&D Center Prof. Sang Joon Lee (Department of Mechanical Engineering, POSTECH) Prof. Il Doo Hwang (Department of Life Sciences, POSTECH) Dr. Sung Sook Ahn Joong Hyuk Park Jeong Eun Ryu Synchrotron facilities PAL, Pohang SSRF, Shanghai PF, Tsukuba

×