Poster59: Identification of waterlogging tolerant Brachiaria hybrids


Published on

Poster for CIAT 2009 Knowledge Sharing Week

Published in: Technology, Business
  • Be the first to comment

  • Be the first to like this

No Downloads
Total views
On SlideShare
From Embeds
Number of Embeds
Embeds 0
No embeds

No notes for slide

Poster59: Identification of waterlogging tolerant Brachiaria hybrids

  1. 1. Centro Internac ional de Agric ultura T International Center for T ropic al ropic al Agric ulture Identification of waterlogging tolerant Brachiaria hybrids Joisse Rincón, John Miles and Idupulapati Rao International Center for Tropical Agriculture (CIAT), A.A. 6713, Cali, Colombia (E-mail: Introduction Results 10 Mean = 7.68 CIAT 6133 LSD 0.05 = 2.09 CIAT 26110 BR05 1609 8 Green leaf biomass (g pot-1) CIAT 6133 CIAT 679 CIAT 606 6 BR04 2069 BR06 0850 BR05 1469 BR02 1794 4 BR05 1609 BR06 1000 Mean = 1.96 CIAT 6294 2 LSD 0.05 = 1.51 CIAT 36061 Check cultivar Tolerant hybrid Sensitive hybrid CIAT 36087 0 MX02 03641 BR05 0334 BR05 1469 Figure 2 . Influence of waterlogging on shoot growth at Material and Methods harvest time (15 days of waterlogging treatment). One 0 2 4 6 8 10 12 14 check cultivar, tolerant hybrid and sensitive hybrid. Dead leaf biomass (g pot-1) Two trials were conducted outside in Forages patio area of CIAT Palmira during 2008 to determine 100 Figure 4. Relationship between green leaf biomass and Mean = 34.5 CIAT 6133 differences in tolerance to waterlogging among 79 LSD0.05 = 5.46 CIAT 679 dead leaf biomass for 79 Brachiaria genotypes under Brachiaria genotypes (5 hybrids of BR02NO series; 8 waterlogging treatment for 15 days. Brachiaria Green leaf biomass proportion (%) 80 hybrids of BR04NO series; 20 hybrids of BR05NO genotypes that developed greater green leaf biomass series; 10 hybrids of MX02NO series; 28 hybrids of CIAT 26110 and lower dead leaf biomass were identified in the BR06NO ; 3 parents - CIAT 606; B. Ruz 44-02; CIAT 60 CIAT 606 upper, left hand quadrant. 6294; and 5 checks - CIAT 679; CIAT 6133; CIAT BR04 2069 26110; CIAT 36061; CIAT 36087). Waterlogging 40 BR06 0850 BR05 01609 treatment was imposed by applying excessive Mean = 20.61 CIAT 6294 BR02 1794 BR06 1132 Table 1. Correlation coefficients (r) between green leaf water to the pots (5 cm over soil surface) for 15 LSD0.05 = 13.78 BR04 3207 biomass proportion (%) and other shoot traits of 79 days. The two trials were planted as randomized 20 Bruz 4402 Brachiaria genotypes under waterlogging in an Oxisol complete block with seven replications. Each CIAT 36061 from Santander de Quilichao. CIAT 36087 experimental unit consisted of one pot filled with 0 BR05 0334 MX02 03641 3.5 kg of fertilized top soil (0-20 cm) from Santander BR05 01469 de Quilichao’s Oxisol and sown with two Plant traits Waterlogging 20 25 30 35 40 45 50 vegetative propagules (stem cuttings). An Green leaf biomass (g pot-1) 0.91*** adequate amounts of fertilizer were supplied to the Total chlorophyll content (SPAD) Total chlorophyll content (SPAD) 0.48*** soil at the time of planting. Plants grew for 60 days Green leaf area (cm2 pot-1) 0.81*** under 100% field capacity of soil moisture. Leaf Figure 3. Relationship between green leaf biomass proportion and the total chlorophyll content for 79 Dead leaf biomass (g pot-1) -0.66*** chlorophyll content (in SPAD units) and leaf Brachiaria genotypes under waterlogging. Brachiaria Stem biomass (g pot-1) 0.36*** photosynthetic efficiency (Fv/Fm) were measured genotypes that developed greater green leaf biomass Leaf photosynthetic efficiency (Fv/Fm) 0.44*** at weekly intervals during the treatment. At the proportion and total chlorophyll content were identified end of the 15 days of treatment, green leaf area in the upper, right hand quadrant. (cm2 pot-1), green leaf biomass (g pot-1), dead leaf *, **, *** Significant at the 0.05, 0.01 and 0.001 probability levels, respectively. biomass (g pot-1) and stem biomass (g pot-1) were measured and green leaf biomass proportion to At 7 days after of establishment of waterlogging total shoot biomass was determined. stress, the majority of plants turned chlorotic. At 15 days after waterlogging treatment, several hybrids Waterlogging Phenotypic Harvest were dead. Two checks, B. humidicola CIAT 6133 and treatment differences CIAT 679 were outstanding in total chlorophyll content, green leaf biomass proportion to total shoot Conclusions biomass and had lower dead leaf biomass (Figures 3 c. and 4). Three hybrids BR04NO/2069, BR06NO/0850 and BR05NO/1609 were superior in their production of Identified three hybrids (BR04NO/2069, a. b. d. green leaf biomass (Figure 4). Two other hybrids BR06NO/0850 and BR05NO/1609) that were superior Physiological variables Waterlogging for two c. Stem, green BR04NO/3207 and BR06NO/1000 showed moderate to the other hybrids in their tolerance to a. Chlorophyll content leaf, dead leaf b. Photosynthetic efficiency weeks biomass. level of tolerance to waterlogging based on green waterlogging. d. Leaf area. leaf biomass proportion to total shoot biomass (Figure 3). Among checks the hybrid cv. Mulato 2 (CIAT This study clearly showed that there is marked Figure 1. Methodology for screening Brachiaria 36087) showed lower level of waterlogging tolerance. genotypic variation in waterlogging tolerance in genotypes under waterlogging conditions. Brachiaria and we could quantify the differences in waterlogging tolerance based on four plant traits including green leaf biomass, green leaf area, leaf References chlorophyll content, dead leaf biomass and green leaf biomass proportion to total shoot biomass. Baruch Z. 1994. Responses to drought and flooding in tropical forage grasses. Plant and Soil 164: 87-96. Días-Filho M. B. 2001. Tolerance to flooding in five Brachiaria brizantha accessions. Pesq. Agropec. Bras., Brasilia, 37: 439-447. Acknowledgements J. Rincón, R. Garcia, J.W. Miles and I. M. Rao (CIAT). 2007. Genotypic variation in waterlogging tolerance of preselected sexual hybrids of Brachiaria. In: Tropical Grasses and Legumes: Optimizing genetic diversity for multipurpose use (Project IP5). Annual report 2007. CIAT. Cali, Colombia, pp 46-47. This work is partially supported from the funds of I. M. Rao, J. Rincón, R. Garcia, J. Ricaurte and J.W. Miles (CIAT). 2007. Screening for tolerance to waterlogging in Brachiaria hybrids. Poster paper presented at the international annual meeting. ASA-CSSA-SSSA. 2007. Grupo Papalotla, Mexico.