Mapping QTL for climbing ability and component traits
in common bean (Phaseolus vulgaris L.)
Oscar E. Checa1 and Matthew W. Blair2 
1/ Universidad de Nariño, Cuidad Universitaria Torobajo, Pasto, Colombia
2/ CIAT - International Center for Tropical Agriculture, A. A. 6713, Cali, Colombia,
Introduction Figures and Tables
Table 1. Means for parents G2333 and G19839 and their ranges Table 2. Genetic correlations among traits in the G2333 x G19839
Plant height - 45 dap (m) in RILs, for components of climbing ability. Mean of 2 reps ± SE. population under high (HP) and low phosphorus (LP) and at 3 sites.
Common bean (Phaseolus vulgaris L.) varies in growth 40 40 40 Components Parents Range TRAIT SITE IL PH1 PH2 CA1 CA2
of Climbing Sites G19839 G2333 Ppar.1 RILs PRIL Plant Darién HP 0.95 **3
habit from aggressive climbing types to bush beans. 30
20
G19839
G2333
30
20
G19839 G2333 30
20
Ability
Plant Darién HP 0.85 ± 0.01 1.96 ± 0.22 *** 0.49 – 2.15 ***
Height
45 DAP
Darién LP
Popayán
0.98
0.71
**
**
G19839 G2333
Height Darién LP 0.51 ± 0.05 1.37 ± 0.04 *** 0.33 – 1.70 ***
Growth habit is determined by a combination of factors
(PH1) Palmira -
Genotype
10 10 1994 1995 10 1996 1997 45 DAP 2 Popayán 0.91 ± 0.06 2.33 ± 0.08 *** 0.73 – 2.32 ***
(m) Palmira 3 - - - Plant Darién HP 0.95 **
0.98 **
0 0 0 ** **
including determinate versus indeterminate growth, total
Plant Darién HP 1.28 ± 0.13 2.53 ± 0.13 *** 0.97 – 3.08 *** Height Darién LP 0.99 0.87
5.2b Darién - LP 6.7b ± 0.53
± 0.57 11.6c ±Popayán 12.5b ± 0.68
0.33 0.55 0.77 0.99 1.21 1.43 1.65 1.87 2.09 0.33 0.55 0.77 0.99 1.21 1.43 1.65 1.87 2.09 0.73 0.93 1.13 1.33 1.53 1.73 1.93 2.13
Mean 10 most resistant RILs
Darién - HP
0.84 Height Darién LP 0.97 ± 0.02 1.78 ± 0.06 ns 0.55 – 2.48 ** 75 DAP Popayán 0.62 **
**
0.92 **
75 DAP Popayán 1.83 ± 0.02 2.80 ± 0.13 *** 1.14 – 3.10 *** (PH2) Palmira 0.99 -
plant height, degree of branching and internode length. Mean 10 most suscep tible RILS s 38.0a ± 0.96 dap (m) 1.08
Plant height - 75 34.2a ± 42.0b ± 1.56 55.6a ± 1.60
(m)
Climbing
Ability
Palmira
Darién HP
Darién LP
0.71 ± 0.03
6.75 ± 0.01
6.45 ± 0.06
1.65 ± 0.06
2.95 ± 0.36
4.00 ± 0.14
ns
***
*
0.46 – 2.10
1.50 – 6.50
2.00 – 7.00
ns
***
ns
Climbing
Ability
Darién HP
Darién LP
-0.82
-
**
-0.85
-
**
-0.93
-
**
Together these factors make up climbing ability. The 40 40 40 Popayán 6.95 ± 0.07 3.27 ± 0.07 *** 1.66 – 6.33 *** ** ** **
45 DAP 45 DAP Popayán -0.74 -0.99 -0.82
44.8a ± 2.84 35.6a ± 3.19 30 56.3a ± 2.80 61.2a ± 2.78
** **
(scale) Palmira 7.50 ± 0.14 4.65 ± 0.21 *** 3.75 – 8.25 ** (CA1) Palmira -0.95 - -0.96
‘Jamapa’ (susc eptible parent)
30 G19839 G2333
30 G19839 G2333 Climbing Darién HP 5.55 ± 0.07 2.95 ± 0.35 *** 1.50 – 6.00 ***
objective of this research was to determine the
** ** ** **
20 20 20 Ability Darién LP 6.25 ± 0.07 3.90 ± 0.01 *** 2.00 – 7.00 ns Climbing Darién HP -0.83 -0.85 -0.95 0.99
G19839 G2333
Popayán 4.70 ± 0.01 2.15 ± 0.07 *** 1.66 – 6.33 *** Ability Darién LP - - - -
1.1b ± 0.45 4.0b ± 0.74 10 9.1c ± 0.82 6.6b ± 1.31
75 DAP
J 117 (resistant parent)
10 10 (scale) Palmira 6.95 ± 0.07 4.50 ± 0.02 * 2.50 – 8.00 ** 75 DAP Popayán -0.67 **
-0.99 **
-0.94 **
0.98 **
quantitative trait loci (QTL) controlling climbing ability in 0
0.55 0.87 1.19 1.51 1.83 2.15 2.47 2.79
0
0.55 0.87 1.19 1.51 1.83 2.15 2.47 2.79
0
1.14 1.39 1.64 1.89 2.14 2.39 2.64 2.89
Internode
length
(cm)
Darién HP
Darién LP
Popayán
7.00 ± 0.94
5.90 ± 0.03
12.6 ± 0.15
18.53 ± 0.24
13.81 ± 1.80
20.21 ± 0.51
***
***
***
6.75 – 23.25
5.00 – 21.50
8.33 – 24.16
***
***
***
(CA2)
Branch
Palmira
Darién HP
-0.96
-
**
-
- -0.95
-
**
0.93
-
**
a population derived from an inter-gene pool cross of an Darién - HP Darién - LP Popayán
Branch
Palmira
Darién HP
7.50 ± 0.14
1.57 ± 0.46
12.89 ± 0.80
1.80 ± 0.27
*
ns
6.50 – 16.30
0.75 – 2.75
*
ns
number
(BN)
Darién LP
Popayán
0.25
0.67
ns
**
0.19
0.70
ns
**
0.15
0.40
ns
**
-
-0.67 **
-
-0.63 **
Climbing ability- 45 dap (1-9 scale) number Darién LP 1.01 ± 0.10 1.24 ± 0.05 ns 0.50 – 2.50 ** Palmira - - - - -
aggressive indeterminate climbing bean with type IV 40 40
Popayán
Palmira
2.61 ± 0.45
1.42 ± 0.10
4.30 ± 0.04
2.67 ± 0.42
*
***
0.61 – 3.58
0.46 – 1.77
***
ns
40 G2333
growth habit (G2333) by an indeterminate bush bean of 30 30
G2333
30
Table 3. QTL for plant height (PH), internode length (IL), climbing ability (CA) and branch number (BN) in the G2333 x G19839 RIL
type IIb growth habit (G19839). 20 20 20 G2333
G19839 G19839 G19839 population detected at either 45 or 75 days after planting in four field experiments (Darién under high phosphorus (HP) or low phosporus
10 10 10 (LP), Popayán (Pop) and Palmira (Pal).
0 0 0
1.5 2.2 2.9 3.5 4.2 4.9 5.6 6.3 6.9 3.8 4.3 4.9 5.4 6.0 6.6 7.1 7.7 8.2 3.3 3.8 4.3 4.8 5.3 5.8 6.3 6.8
Darién HP Palmira Popayán Trait –
Materials and Methods
Evaluation Site QTL Linkage Closest Marker LR R2 Total R2 Additivity
Climbing ability- 75 dap (1-9 scale) Date1 name group marker interval
G2333
40 40 40
30 30 30 G2333 Plant height
G2333
Plant Material and Experimental Design: A total of 84 F5:8 20 G19839 20
G19839
20
G19839
PH-1
PH-1
HP
Pop
Plh1-2
Plh1-1
B04
B03
X010.85
BM189
L040.75- BMd027
BM189 - BMd036
22.82
15.11
0.1952
0.1399
0.4812
0.5119
0.1903
0.1358
10 10 10
recombinant inbred lines were used in sole-crop, randomized 0
1.5 2.2 2.9 3.5 4.2 4.9 5.6 6.3 6.9
0
2.5 3.2 3.9 4.6 5.3 6.0 6.6 7.3
0
1.7 2.2 2.8 3.4 4.0 4.6 5.1 5.7 6.3
PH-1 Pop Plh1-3 B04 PV-ctt001 PV-ctt001 - BM161 16.14 0.1403 0.5276 0.1395
PH-1 Pop Plh1-4 B08 M130.7 BM211 - X011.25 15.41 0.1055 0.4762 0.1233
complete block design experiments with two replications that Darién HP Palmira Popayán
PH-2 HP Plh2-1 B04 PV-ctt001 AK060.55 - BM161 20.53 0.2489 0.4158 0.2523
were planted in Darién (1,485 masl), Palmira (965 masl), and Internode length (cm) Branch number PH-2 LP Plh2-3 B11 BMd033 BMd033 - X010.5 15.35 0.1637 0.3039 0.1887
PH-2 Pal Plh2-2 B04 BMd026 L040.75 - X010.85 16.90 0.1445 0.4624 0.1255
Popayán (1,730 masl). In Darién, the two trials were planted 30 40 50
under conditions of high (64.3 ppm) and low phosphorus (1.7 20
G19839
G2333
30
G19839
G2333
40
30
G19839
Internode length
IL HP Int2 B04 X010.85 BMd068 - BMd027 23.69 0.1839 0.5407 1.8164
20
ppm), respectively. 10
10
20
10
G2333 IL HP Int3 B04 PV-ctt001 AK060.55 - BM161 15.97 0.2033 0.453 1.9515
IL Pal Int4 B04 L040.75 L040.75 - OAP7 22.52 0.2836 0.4067 1.1886
0 0 0
Trait Measurements: Field data were collected for climbing 3.8 6.2 8.6 11.1 13.5 16.0
Darién HP
18.4 20.8 3.8 6.2 8.6 11.1 13.5 16.0 18.4 20.8
Darién LP
0.5 0.8 1.1 1.3
Darién LP
1.6 1.9 2.2 2.5 2.7 IL
IL
Pal
Pal
Int2
Int3
B04
B04
BMd026
PV-ctt001
OU14 - X010.85
AK060.55 - BM161