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Evaluation of Brachiaria humidicola germplasm
accessions for tolerance to waterlogging
Joisse     Rincón1,       Juan Andr...
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poster21: Evaluation of Brachiaria humidicola germplasm accessions for tolerance to waterlogging

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poster21: Evaluation of Brachiaria humidicola germplasm accessions for tolerance to waterlogging

  1. 1. Evaluation of Brachiaria humidicola germplasm accessions for tolerance to waterlogging Joisse Rincón1, Juan Andrés Cardoso1,2, John Miles 1 and Idupulapati Rao 1 1Centro Internacional de Agricultura Tropical (CIAT), A.A. 6713, Cali, Colombia 2University of Granada, 18071 Granada, España E-mail: j.d.rincon@cgiar.org In tropical pastures periods of excess soil water can be relatively common  Location and date: A pot experiment was conducted during October 2009, at the Forages events. Usually, excess water in pasture soils (waterlogging or flooding) is a patio area (CIAT, Cali, Colombia). short-term event and can be caused by the combination of intensive rains  Genotypes: 66 germplasm accessions of B. humidicola with 7 checks and low soil drainage (e.g., soils with a drainage impediment layer) (Dias- (BR04/3207,BR05/1738, BR06/1000, CIAT 26110, CIAT 36087, CIAT Filho, 2008). 16843, Brachipara) Currently, Brachiaria pastures are the most widely sown pastures in Latin  Experimental design: Randomized complete blocks with 4 America. Climate change will increase the risk for these pastures for replications. seasonal waterlogging.  Plant culture: Two stem cuttings per pot filled with 1.3kg of a fertilized top soil (Oxisol) from CIAT´s Brachiaria breeding program seeks to incorporate in Brachiaria Santander de Quilichao. hybrids attributes associated with adaptation to climate change (drought Treatment: After two months of growth, soil was waterlogged with and excessive soil moisture), thereby to maintain or increase the a 5cm water lamina over the soil. Drainage of water was prevented productivity of Brachiaria pastures in the tropics. Since 2005 CIAT has been using a plastic bag outside the pot and maintained in position by inserting the plastic covered using a methodology for assessing waterlogging tolerance in Brachiaria pot into another pot of the same size. genotypes. The objectives of this study were:  Harvest: After 30 days of waterlogging treatment, plants were harvested by separating into  To evaluate phenotypic differences in waterlogging tolerance among 66 green leaves, dead leaves and the remaining shoot material. germplasm accessions of B. humidicola along with 7 checks. Measured plant attributes:  Identify physiological and morphological traits that could be used as During growth Harvest Others indicators of waterlogging tolerance in Brachiaria. • Leaf chlorophyll content (SPAD) • Leaf area (cm2 pot-1) • Green leaf biomass proportion (%) • Photosynthetic efficiency in • Dead leaf biomass, Green leaf • Visual evaluation light adapted leaves (fv’/fm’) biomass, Stem biomass (g pot1) After 30 days of waterlogging treatment, differences in tolerance to waterlogging were observed among the genotypes tested. Four genotypes (CIAT 16873, CIAT 6133, CIAT 26371 and CIAT 6707) were found to be outstanding in their ability to withstand waterlogging stress since these genotypes maintained a higher proportion of green leaf biomass compared to total leaf biomass. The check genotype CIAT 36087 (Mulato 2 ) showed the lower green leaf biomass proportion (Figure 2A). Two other checks (CIAT 26110 and Brachipara) showed an intermediate level of waterlogging tolerance (Figure 2A). 6 50 Mean = 59 CIAT 16843 A LSD0.05 = 14.18 CIAT 16873 CIAT 6133 B CIAT 16892 CIAT 16891 Leaf chlorophyll content (SPAD) 5 CIAT 26371 CIAT 16890 CIAT 26411 Green leaf biomass (g pot -1) CIAT 6707 40 CIAT 26371 CIAT 26573 CIAT 26159 CIAT 26152 CIAT 675 CIAT 6707 CIAT 6705 CIAT 26110 CIAT 26312 CIAT 16887 CIAT 26570 Mean = 30.37 CIAT 16880 CIAT 16873 4 CIAT 26159 CIAT 6013 LSD0.05 = 9.20 CIAT 6013 CIAT 679 CIAT 6369 CIAT 682 CIAT 26570 CIAT 26575 CIAT 675 CIAT 679 CIAT 26110 30 CIAT 26438 CIAT 16876 CIAT 24146 CIAT 6133 CIAT 6705 3 Mean = 2.48 CIAT 16888 CIAT 16886 CIAT 16843 LSD0.05 = 0.97 BRACHIPARA CIAT 26312 CIAT 36087 BR06 /1000 CIAT 16180 CIAT 16843 20 BR04 /3207 BRACHIPARA 2 BR04 /3207 BR05 /1738 BR05 /1738 CIAT 26145 CIAT 26110 BR06 /1000 CIAT 16879 10 CIAT 16180 1 CIAT 16882 CIAT 36087 Intermediate Susceptible Susceptible CIAT 36087 CIAT 26312 Mean = 59 Tolerant check Tolerant genotype check genotype check LSD0.05 = 14.18 0 0 Figure 1. Phenotypic differences among Brachiaria genotypes under waterlogged 20 30 40 50 60 70 80 20 30 40 50 60 70 80 conditions. Green leaf biomass proportion (%) Green leaf biomass proportion (%) Leaf chlorophyll content showed a significant positive correlation with green leaf biomass proportion Figure 2. A. Relationship between green leaf biomass proportion and (r2= 0.42), thus indicating that this is a physiological trait that can be used as a tool to evaluate green leaf biomass for 73 Brachiaria genotypes grown under waterlogging differences of waterlogging tolerance among genotypes of Brachiaria (Figure 2B). The genotype that conditions. B. Relationship between green leaf biomass proportion and showed the highest value of total biomass after 30 days of waterlogging treatment was the tolerant leaf chlorophyll content (SPAD). Brachiaria genotypes that showed greater values of green leaf biomass, leaf chlorophyll content and green leaf check CIAT 16843 (Brachiaria arrecta) and the one with the lower total biomass was Brachiaria biomass proportion were identified in the upper, right hand quadrant. humidicola CIAT 26312 (Figure 3). 35 Stem; LSD O.O5 = 3.12 Figure 3 . Influence of waterlogging on 30 Dead leaf; LSD O.O5 = 0.99 genotypic variation in shoot biomass of Shoot biomass (g pot-1) Green leaf; LSD O.O5 = 0.97  Identified four Brachiaria humidicola (CIAT 16873, CIAT 6133, CIAT 25 66 Brachiaria humidicola germplasm 26371 and CIAT 6707) germplasm accessions that were superior to accessions and 7 checks. LSD values are the other genotypes in their tolerance to waterlogging. at the 0.05 probability level. 20  Green leaf biomass, leaf chlorophyll content and green leaf biomass 15 proportion are the three variables that can be used as selection criteria for evaluating waterlogging tolerance in Brachiaria. 10 5 0  Caetano L. P and M. B. Días-Filho. 2008. Responses of six Brachiaria spp. accessions to CIAT 6013 CIAT 675 CIAT 679 CIAT 6133 CIAT 682 CIAT 6705 CIAT 6738 CIAT 6707 CIAT 6709 CIAT 6369 CIAT 16843 CIAT 26570 CIAT 16873 CIAT 26371 BRACHIPARA CIAT 26375 CIAT 26414 CIAT 26438 CIAT 26575 CIAT 26415 CIAT 26573 CIAT 26154 C IAT 16890 CIAT 26159 CIAT 26416 CIAT 16894 CIAT 16350 CIAT 26110 CIAT 26425 CIAT 16868 CIAT 16887 CIAT 26427 BR04 /3207 CIAT 16886 CIAT 26411 CIAT 16867 CIAT 16876 CIAT 16181 CIAT 16888 CIAT 26430 CIAT 16889 CIAT 16883 CIAT 24146 CIAT 16878 CIAT 16866 CIAT 26160 CIAT 36083 CIAT 26152 CIAT 16885 CIAT 26155 CIAT 26407 CIAT 16180 CIAT 16874 CIAT 16892 CIAT 16877 CIAT 16880 CIAT 16891 BR05 /1738 CIAT 16870 CIAT 16882 CIAT 26413 CIAT 26151 CIAT 16871 CIAT 26145 CIAT 26149 BR06 /1000 CIAT 16182 CIAT 16183 CIAT 16884 CIAT 36087 CIAT 26181 CIAT 16879 CIAT 26312 root zone flooding. R. Bras. Zootec. 37: 795-801.  Rincón, J., R. Garcia, J.W. Miles and I. M. Rao. 2008. Genotypic variation in waterlogging tolerance of 71 promising Brachiaria hybrids. In: Improved multipurpose forages for the Genotype developing world.Annual report 2008. CIAT. Cali, Colombia, pp 49-53 .  Rao, I. M., J. Rincón, R. Garcia, J. Ricaurte and J.W. Miles. 2007. Screening for tolerance ACKNOWLEDGEMENTS to waterlogging in Brachiaria hybrids. Poster paper presented at the international annual meeting ASA-CSSA-SSSA. 2007. This work is supported from the funds of FONTAGRO

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