The identification of Larvae, eggs and stomach contents… three studies of the application of barcodes on Mexican fish. Martha Elena Valdez Moreno Lourdes Vásquez Yeomans Carolina Quintal Lizama Dalia Cázarez Carrillo Elva Leyva
Contoy Punta Allen Contoy B. Chinchorro Xcalak Mahahual Banco ChinchorroTheMexicanCaribbean Xcalak
There exist many places of reeffish spawning aggregations andnursery areas of economic andecological importance. To ensure the sustainability of these areas as integrated ecosystems and the possibility to protect them, it is essential to know…
What fish species are living in this region? Where are they? Adults Juvenile 74 mm LT 22 mm LT Larvae ? 19 mm LT Eggs 7 mm LT
Applying barcodeswe have found some interesting things…
Barcodes help us to identify the larvae…69 new records for the Mexican Caribbeanonly at larvae stage Awaous banana Pterois volitans* River goby Lionfish Cozumel
766 adult species with their larvae, previously unknown.Family GerreidaeGenus Eucinostomus E. jonesii E. melanopterus E. gula Using barcode The external morphology is not useful to identify these larvae…..
Barcode allows us to know the distribution and thetime of the appearance of larvae species economically important 8-29/march/06 19/march/07 Mycteroperca bonaci Black grouper Aug/2005 Thunnus atlanticus Mar/2006 Blackfin tuna
7Family GerreidaeGenus Eucinostomus E. jonesii E. melanopterus E. gula Jan-AugAll year Mar-may Xcalak
Egg distribution and the time of the appearance …
Regalecus glesne King of herrings Cozumel 9-Apr-2009 this fish could approach the coast in the north ofthe largest teleost fish of the world (8-10 m) the Mexican Caribbean for spawning or feeding
Lachnolaimus maximus Hogfish Xcalak adults 1-30 Jan-2007
The case of…Mycteroperca bonaci Life cycle of the species when and where the fishes spawn development and maintenance fisheries propose protected areas
There are several reasons to beconcerned about their presence:They have venomous spines that could Is the first non-injure divers. native marine fish established in the Western North Atlantic andMexico has many places that are Caribbean Sea.world class scuba diving destinationLionfish are predators that consume“mexican” native reef species!!! What kind of prey do they eat in the mexican caribbean ?
Stomach contents analysis Cozumel, Xcalak,Contoy island122 Pterois volitans Banco Chinchorro,Puerto Morelosfixed in alcohol (2009) Playa del Carmen 35 fresh digestive tracts were Mahahual placed in ethylic alcohol 96% Xcalak (2010) 90% showed a high degree of digestion of the tissue A total of 157 stomachs were examined only 144 had stomach contents We separated 330 prey items
Results 170 sequences were compared with a reference library of DNA barcodes. 125 sequences matched with fishes 45 sequences matched with crustacean
Fish: 30 different species were identified (12 families) 5 new records for Mexican Caribbean 1 surprise…. Gobidae* Apogonidae* Coryphopterus eidolon C. hyalinusApogon townsendi Apogon maculatus C. venezuelae C. thrix Apogon lachneri Astrapogon puncticulatus C. tortugae Apogon mosavi Priolepis hipoliti Lythypnus sp *greatest number of species
Crustacean45 sequencesUsing the barcodes wecould identify 20 differentspecies.3 Orders:Euphausiacea…..1 speciesStomatopoda……2 speciesDecapoda………17 species
Euphausiacea Euphausia americana Euphausia spStomatopoda Pseudosquilla sp. This only was able to assign to family level… Gonodactylidae…
Decapoda: 17 species Hyppolytidae Thor sp. Alpheidae (CRU120) Palaemonidae Something like this….
In BoldDecapoda: 141 Familiesand 2,049 sp sequencedThe BOLD data base is notcomplete yet….
Conclusions1. This is an example of how important the barcodes are in the support of sustainable fisheries.2. With the help of barcodes we will know when and where the fish spawn, by directing protection measures in these particular seasons and sites we well suport fisheries.3. This is the first time we have a tool that will help us to identify all species targeted by lion fish.4. This is the first time that we have been able to report the kind of prey the lionfish consumes in the Mexican Caribbean.5. The only limit has been that the barcode library is still incomplete, mainly crustaceans, but with time it will be possible to identify all species consumed, not only by exotics, but also by any other species.
Acknowledgements:NOAA y RSMASCONANP – Xcalak y Banco ChinchorroCONABIO y BARCODE of LIFEECOSURITCH Jacc Diana Manuel Caro Lulú Selene Paty
Thanks for your attention!We need to visitthese places…. Xcalak