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Re-collection to assess temporal variation in wild barley diversity in Jordan

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Presentation delivered by Dr Imke Thormann at the International Agrobiodiversity Congress 2016, held in Delhi, India, 6-9 November.
Imke Thormann's presentation focused on crop wild relative genetic erosion and how it can be studied.
Find out more about the India Agrobiodiversity Congress:
http://www.bioversityinternational.org/iac2016/

Published in: Science
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Re-collection to assess temporal variation in wild barley diversity in Jordan

  1. 1. Re-collection to assess temporal variation in wild barley diversity in Jordan Thormann I, Engels JMM, Börner A, Lohwasser U, Pillen K, Richards CM 1st International Agrobiodiversity Congress, 6-9 November 2016, New Delhi, India
  2. 2. Genetic erosion studies on Crop Wild Relatives How changes the diversity over time? Where has crop wild relative diversity been lost? What factors drive change and loss? INTRODUCTION
  3. 3. Germplasm collections as resource Plenty of germplasm collecting during the past century Conserved in genebanks around the world Snapshots of diversity Useful as baseline One example: Bioversity International Collecting Database http://bioversity.github.io/geosite/INTRODUCTION
  4. 4. Bioversity International Collecting Database Over 220,000 samples collected in over 1000 collecting trips between 1975 and 2012 (90% collected between 1975-1995) 27% are wild species 25% of trips collected only wild species Location data and mission reports available INTRODUCTION http://bioversity.github.io/geosite/
  5. 5. Re-collection of barley in Jordan: 1981 / 2012 METHODS Verification with Google earth and gazetteers • 1981 seed samples conserved in NordGen • Set of wild barley population pairs collected from same 18 sites in both years
  6. 6. Data and analyses METHODS • Field trial in IPK • 16 individual plants per population • 38 SSRs for genetic diversity, differentiation and population structure analyses • 10 variable phenotypic traits for assessment of relative changes in phenotypes
  7. 7. Data and analyses METHODS • Trends in temperature and rainfall variables; differences between collecting time points • Correlations between climatic, phenotypic and genetic change, and with geographical variables (latitude and elevation). VariableYear 1980 1981 … 2012 2013 Annual precipitation Driest month Driest quarter Wettest month Wettest quarter Annual mean temp Hottest month Hottest quarter Coldest month Coldest quarter • Analogue to Bioclim variables 1, 5, 6, 10-14, 16, 17
  8. 8. RESULTS 0 0.5 1 1.5 2 2.5 3 7575 7561 7572 7586 7588 7640 7599 7600 7595 7594 7596 7602 7616 7604 7617 7606 7612 7628 Change in annual, quarterly and monthly temperature means between collecting periods per site Change annual temp Change warmest quarter Change coldest quarter Change warmest month change coldest month Annual temperature
  9. 9. RESULTS • Population structure largely maintained • Higher genetic diversity in 2012 (no. of alleles, allelic richness, no. of multi-locus genotypes) • Lower differentiation among populations in 2012 (lower FST, increased genetic and physical admixture).
  10. 10. RESULTS • Phenotype variation within and among sites comparable in both collecting years, confirming high variability observed in other studies • Relative change in phenotype among collecting years (further field trials) • No correlations (except change in climate with latitude)
  11. 11. Summary RESULTS • Observed pattern of increased diversity, reduced differentiation and maintenance of phenotypic variation: evidence of an additional possible response in CWR exposed to a variable and changing environment. • Reduced differentiation indicating increased seed dispersal, likely due to the intensification of agriculture and grazing. •  include agricultural practices and seed flow in studies on CWR growing in disturbed habitats and agricultural areas. • Useful approach to investigate temporal changes in CWR diversity • Bioversity International Collecting Database: rich source of baseline data for similar studies.
  12. 12. Acknowledgements, references • National Center for Agricultural Research and Extension (NCARE), Jordan • International Center for Agricultural Research in Dry Areas (ICARDA) • National Center for Genetic Resources Preservation (NCGRP), USDA, USA • German Federal Genebank, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) • Bioversity International. Thormann I, Reeves P, Thumm S, Reilley A, JMM Engels, Lohwasser U, Börner A, Pillen K, Richards CM (2016) Genotypic and phenotypic changes in wild barley (Hordeum vulgare subsp. spontaneum) during a period of climate change in Jordan. Genetic Resources and Crop Evolution. DOI: 10.1007/s10722-016-0437-5. Thormann I, Fiorino E, Halewood M, Engels JMM (2015) Plant genetic resources collections and associated information as baseline resource for genetic diversity studies – an assessment of the IBPGR supported collections. Genetic Resources and Crop Evolution 62(8):1279-1293.
  13. 13. Thank you www.bioversityinternational.org/subscribe @BioversityInt Imke Thormann i.thormann@cgiar.org

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