iGEM 2013 NTU-Taida Presentation Part 2 修改後範例

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iGEM 2013 NTU-Taida Presentation Part 2 修改後範例

  1. 1. Overview Circuit Result Modeling Human Practice
  2. 2. Three Circuit Types GFP 1.Original GFP GFP GFP 2.Positive Feedback Faster & Higher Intensity GFP GFP GFP 3.Negative Regulation More Stable Overview Circuit Result Modeling Human Practice
  3. 3. 1.Original Circuit Induced Promoter RBS Reporter Double Terminator Constitutive Promoter QS Receptor GFP Overview Circuit Result Modeling Human Practice Double Terminator
  4. 4. 1.Original Circuit Induced Promoter RBS Reporter Double Terminator Constitutive Promoter QS Receptor GFP Overview Circuit Result Modeling Human Practice Double Terminator
  5. 5. 1.Original Circuit Induced Promoter RBS Reporter Double Terminator Constitutive Promoter QS Receptor GFP Overview Circuit Result Modeling Human Practice Double Terminator
  6. 6. 1.Original Circuit Induced Promoter RBS Reporter Double Terminator Constitutive Promoter QS Receptor GFP Overview Circuit Result Modeling Human Practice Double Terminator
  7. 7. 1.Original Circuit Induced Promoter RBS Reporter Double Terminator Constitutive Promoter QS Receptor GFP Overview Circuit Result Modeling Human Practice Double Terminator
  8. 8. 1.Original Circuit Induced Promoter RBS Reporter Double Terminator Constitutive Promoter QS Receptor GFP Overview Circuit Result Modeling Human Practice Double Terminator
  9. 9. 1.Original Circuit Induced Promoter RBS Reporter Double Terminator Constitutive Promoter QS Receptor GFP Overview Circuit Result Modeling Human Practice Double Terminator
  10. 10. 1.Original Circuit Induced Promoter RBS Reporter Double Terminator Constitutive Promoter QS Receptor GFP GFP Overview Circuit Result Modeling Human Practice Double Terminator
  11. 11. ELISA Plate Reader Overview Circuit Result Modeling Human Practice
  12. 12. ELISA Plate Reader Overview Circuit Result Modeling Human Practice
  13. 13. Flow Cytometry Start Overview Circuit Result Modeling Human Practice
  14. 14. Flow Cytometry Start 1st Hour Overview Circuit Result Modeling Human Practice
  15. 15. Flow Cytometry Start 1st Hour Overview 2nd Hour Circuit Result Modeling Human Practice
  16. 16. Flow Cytometry Start 1st Hour Overview 2nd Hour Circuit Result 3rd Hour Modeling Human Practice
  17. 17. Flow Cytometry Start 1st Hour Overview 2nd Hour Circuit Result 3rd Hour Modeling 4th Hour Human Practice
  18. 18. 2. Positive Feedback Circuit GFP RhIR pRhI Overview Circuit Result Modeling Human Practice
  19. 19. 2. Positive Feedback Circuit GFP RhIR pRhI Overview Circuit Result Modeling Human Practice
  20. 20. 2. Positive Feedback Circuit RhIR GFP Overview Circuit Result RhIR pRhI Modeling Human Practice
  21. 21. 2. Positive Feedback Circuit RhIR GFP RhIR pRhI RhIR Overview Circuit Result Modeling Human Practice
  22. 22. 2. Positive Feedback Circuit RhIR GFP RhIR pRhI RhIR Overview Circuit Result Modeling Human Practice
  23. 23. 2. Positive Feedback Circuit RhIR GFP RhIR pRhI RhIR RhIR Overview Circuit Result Modeling Human Practice
  24. 24. 2. Positive Feedback Circuit RhIR GFP RhIR pRhI RhIR RhIR Overview Circuit Result Modeling Human Practice
  25. 25. 2. Positive Feedback Circuit RhIR GFP RhIR pRhI GFP RhIR RhIR Overview Circuit Result Modeling Human Practice
  26. 26. 2. Positive Feedback Circuit RhIR GFP RhIR pRhI GFP RhIR RhIR RhIR Overview Circuit Result Modeling Human Practice
  27. 27. 2. Positive Feedback Circuit RhIR GFP RhIR pRhI GFP RhIR RhIR RhIR Positive Feedback Circuit Overview Circuit Result Modeling Human Practice
  28. 28. ELISA Plate Reader Overview Circuit Result Modeling Human Practice
  29. 29. ELISA Plate Reader Overview Circuit Result Modeling Human Practice
  30. 30. Flow Cytometry Start Overview Circuit Result Modeling Human Practice
  31. 31. Flow Cytometry Start 1st Hour Overview Circuit Result Modeling Human Practice
  32. 32. Flow Cytometry Start 1st Hour Overview 2nd Hour Circuit Result Modeling Human Practice
  33. 33. Flow Cytometry Start 1st Hour Overview 2nd Hour Circuit Result 3rd Hour Modeling Human Practice
  34. 34. Flow Cytometry Start 1st Hour Overview 2nd Hour Circuit Result 3rd Hour Modeling 4th Hour Human Practice
  35. 35. 3. Negative Regulation Divided into 3 separated steps: Overview Circuit Result Modeling Human Practice
  36. 36. 3. Negative Regulation Divided into 3 separated steps: Positive Feedback Overview Circuit Result Modeling Human Practice
  37. 37. 3. Negative Regulation Divided into 3 separated steps: Positive Feedback Overview Repressor Circuit Result Modeling Human Practice
  38. 38. 3. Negative Regulation Divided into 3 separated steps: Positive Feedback Overview Repressor Circuit Result Modeling Reporter Human Practice
  39. 39. 3. Negative Regulation Positive Feedback Repressor Reporter Overview Circuit Result Modeling Human Practice
  40. 40. 3. Negative Regulation Double Terminator LasR pConst Positive Feedback Repressor Reporter Overview Circuit Result Modeling Human Practice
  41. 41. 3. Negative Regulation Double Terminator LasR pConst Positive Feedback LasR Repressor Reporter Overview Circuit Result Modeling Human Practice
  42. 42. 3. Negative Regulation Double Terminator LasR pConst LasR Positive Feedback AHL Repressor Reporter Overview Circuit Result Modeling Human Practice
  43. 43. 3. Negative Regulation Double Terminator LasR pConst LasR Positive Feedback AHL LasR CI Repressor pLas Reporter Overview Circuit Result Modeling Human Practice
  44. 44. 3. Negative Regulation Double Terminator LasR pConst LasR Positive Feedback AHL LasR CI Repressor pLas Reporter Overview Circuit Result Modeling Human Practice
  45. 45. 3. Negative Regulation Double Terminator LasR pConst LasR Positive Feedback AHL LasR CI Repressor pLas CI Reporter Overview Circuit Result Modeling Human Practice
  46. 46. 3. Negative Regulation Double Terminator Positive Feedback LasR pConst LasR AHL LasR Repressor CI pLas CI GFP pCI Reporter Luciferase/XFP Overview Circuit Result Modeling Human Practice
  47. 47. 3. Negative Regulation Double Terminator Positive Feedback LasR pConst LasR AHL LasR Repressor CI pLas CI GFP pCI Reporter Luciferase/XFP Overview Circuit Result Modeling Human Practice
  48. 48. 3. Negative Regulation Double Terminator Positive Feedback LasR pConst LasR AHL LasR Repressor CI pLas CI GFP pCI Reporter Luciferase/XFP Overview Circuit Result Modeling Human Practice
  49. 49. 3. Negative Regulation Double Terminator Positive Feedback LasR pConst LasR AHL LasR Repressor CI pLas CI GFP pCI Reporter Luciferase/XFP Overview Circuit Result Modeling Human Practice
  50. 50. ELISA Plate Reader Overview Circuit Result Modeling Human Practice
  51. 51. ELISA Plate Reader Overview Circuit Result Modeling Human Practice
  52. 52. Flow Cytometry Start Overview Circuit Result Modeling Human Practice
  53. 53. Flow Cytometry Start 1st Hour Overview Circuit Result Modeling Human Practice
  54. 54. Flow Cytometry Start 1st Hour Overview 2nd Hour Circuit Result Modeling Human Practice
  55. 55. Flow Cytometry Start 1st Hour Overview 2nd Hour Circuit Result 3rd Hour Modeling Human Practice
  56. 56. Flow Cytometry Start 1st Hour Overview 2nd Hour Circuit Result 3rd Hour Modeling 4th Hour Human Practice
  57. 57. Comparison Of Three Circuits GFP 1.Original GFP GFP GFP 2.Positive Feedback Faster & Higher Intensity GFP GFP GFP 3.Negative Regulation More Stable Overview Circuit Result Modeling Human Practice
  58. 58. Comparison Of Three Circuits Normal State GFP 1.Original GFP GFP GFP 2.Positive Feedback Faster & Higher Intensity GFP GFP GFP 3.Negative Regulation More Stable Overview Circuit Result Modeling Human Practice
  59. 59. Comparison Of Three Circuits QS Signal GFP 1.Original GFP GFP GFP 2.Positive Feedback Faster & Higher Intensity GFP GFP GFP 3.Negative Regulation More Stable Overview Circuit Result Modeling Human Practice

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