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Recurrent Third-Trimester Fetal Loss and Maternal
Mosaicism for Long-QT Syndrome
Todd E. Miller, PhD; Elicia Estrella, MS;...
revealed no gross abnormalities, but a subsequent fetal echocardio-
gram identified frequent bursts of atrial and ventricu...
and her QTc interval did not lengthen abnormally during amiodarone
infusion given for treatment of fetal arrhythmias (not ...
HinfI restriction enzyme site (GANTC3AANTC). As ex-
pected, the wild-type exon 28 PCR product was completely
digested by H...
mias in the fetus and neonate.5,28,29 The poor prognosis of
infants with neonatal LQTS undoubtedly also contributes to
low...
20. Leuer M, Oldenburg J, Lavergne JM, et al. Somatic mosaicism in hemo-
philia A: a fairly common event. Am J Hum Genet. ...
H. Bishopric
Paolo Rusconi, Mary Ellen Ahearn, Lisa Baumbach, Paul Kurlansky, Grace Wolff and Nanette
Todd E. Miller, Elic...
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Recurrent Third-Trimester Fetal Loss and Maternal Mosaicism for Long-QT Syndrome

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Recurrent Third-Trimester Fetal Loss and Maternal
Mosaicism for Long-QT Syndrome

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Recurrent Third-Trimester Fetal Loss and Maternal Mosaicism for Long-QT Syndrome

  1. 1. Recurrent Third-Trimester Fetal Loss and Maternal Mosaicism for Long-QT Syndrome Todd E. Miller, PhD; Elicia Estrella, MS; Robert J. Myerburg, MD; Jocelyn Garcia de Viera, MD; Niberto Moreno, BS; Paolo Rusconi, MD; Mary Ellen Ahearn, MS; Lisa Baumbach, PhD; Paul Kurlansky, MD; Grace Wolff, MD; Nanette H. Bishopric, MD Background—The importance of germ-line mosaicism in genetic disease is probably underestimated, even though recent studies indicate that it may be involved in 10% to 20% of apparently de novo cases of several dominantly inherited genetic diseases. Methods and Results—We describe here a case of repeated germ-line transmission of a severe form of long-QT syndrome (LQTS) from an asymptomatic mother with mosaicism for a mutation in the cardiac sodium channel, SCN5A. A male infant was diagnosed with ventricular arrhythmias and cardiac decompensation in utero at 28 weeks and with LQTS after birth, ultimately requiring cardiac transplantation for control of ventricular tachycardia. The mother had no ECG abnormalities, but her only previous pregnancy had ended in stillbirth with evidence of cardiac decompensation at 7 months’ gestation. A third pregnancy also ended in stillbirth at 7 months, again with nonimmune fetal hydrops. The surviving infant was found to have a heterozygous mutation in SCN5A (R1623Q), previously reported as a de novo mutation causing neonatal ventricular arrhythmia and LQTS. Initial studies of the mother detected no genetic abnormality, but a sensitive restriction enzyme–based assay identified a small (8% to 10%) percentage of cells harboring the mutation in her blood, skin, and buccal mucosa. Cord blood from the third fetus also harbored the mutant allele, suggesting that all 3 cases of late-term fetal distress resulted from germ-line transfer of the LQTS-associated mutation. Conclusions—Recurrent late-term fetal loss or sudden infant death can result from unsuspected parental mosaicism for LQTS-associated mutations, with important implications for genetic counseling. (Circulation. 2004;109:3029-3034.) Key Words: long-QT syndrome Ⅲ genetics Ⅲ genes Inherited forms of the long-QT syndrome (LQTS) have been associated with Ͼ200 different mutations in 7 genes encoding cardiac ion channels, their regulatory subunits, and a membrane anchoring protein.1,2 The vast majority of these mutations are dominant point mutations (Romano-Ward syn- drome) that result in amino acid substitutions or non-sense codons. Current molecular genetic screening protocols can identify potentially causative mutations in approximately two thirds to three fourths of familial cases.3,4 See p 2930 Recently, case reports and an epidemiological study have implicated LQTS in sudden unexplained infant death (sudden infant death syndrome [SIDS]),5–7 but genetic testing for LQTS is not widely available and is infrequently performed in cases of perinatal death. Hence, the extent of the contri- bution of LQTS to sudden infant death remains unknown. When parents are clinically normal, a genetic basis for sudden death may not be suspected but could arise either from de novo mutation in the fetus or through parental germ-line transmission of a mutation. This latter phenomenon is surprisingly common and may underlie up to 30% of what otherwise appear to be de novo cases of dominantly inherited genetic disorders.8–11 Here, we describe the case of a clini- cally unaffected mother with somatic mosaicism for a known pathogenic mutation in the cardiac sodium channel, SCN5A, who transmitted a severe form of LQTS to multiple succes- sive pregnancies. These findings have significant implica- tions for the evaluation of causes of late-term fetal distress and demise. and for genetic counseling of apparently de novo cases of LQTS. Methods Clinical Subjects A 1340-g black male infant was delivered by emergency cesarean section at 32 weeks’ gestation because of fetal arrhythmias and hydrops. Ventricular arrhythmias were initially diagnosed in utero at 28 weeks of gestation. An initial obstetrical ultrasound at 28 weeks Received December 1, 2003; revision received March 2, 2004; accepted March 15, 2004. From the Departments of Medicine (Division of Cardiology) (T.E.M., R.J.M., N.M., N.H.B.), Pediatrics (E.E., J.G.d.V., P.R., M.E.A., L.B., G.W., N.H.B.), and Molecular and Cellular Pharmacology (N.H.B.), University of Miami School of Medicine, and Miami Heart Research Institute (P.K.), Miami Fla. Correspondence to Nanette H. Bishopric, MD, FACC, FAHA, Professor of Pharmacology, Medicine and Pediatrics, Department of Molecular and Cellular Pharmacology (R-189), PO Box 016189, Miami, FL 33101. E-mail n.bishopric@miami.edu © 2004 American Heart Association, Inc. Circulation is available at http://www.circulationaha.org DOI: 10.1161/01.CIR.0000130666.81539.9E 3029at VA MED CTR BOISE on February 10, 2016http://circ.ahajournals.org/Downloaded from
  2. 2. revealed no gross abnormalities, but a subsequent fetal echocardio- gram identified frequent bursts of atrial and ventricular tachycardia and episodes of 2:1 AV block. ␤-Blockers were ineffective, and a trial of amiodarone resulted in marked worsening of arrhythmias, with development of myocardial dysfunction and fetal hydrops necessitating emergency caesarian section. An ECG demonstrated AV conduction abnormalities and a QTc interval of 550 milliseconds (Figure 1A). After delivery, the newborn continued to have drug-re- sistant ventricular tachyarrhythmias, frequent episodes of ventricular flutter, and torsade de pointes (Figure 1B). A chest x-ray was consistent with hyaline membrane disease and mild cardiomegaly. Tachyarrhythmias were partially responsive to lidocaine infusion but resisted a variety of other antiarrhythmic interventions, including epicardial pacing, esmolol infusion, bicarbonate, magnesium, cal- cium, mexiletine, flecainide, and left sympathetic ganglionectomy. Ultimately, the infant received orthotopic heart transplantation at 5.5 months of age. He is now almost 3 years of age and has had no further arrhythmias. The infant’s mother was a 20-year-old black woman with no clinical evidence or family history of heart disease, cardiac arrhyth- mias, prematurity, syncope, cardiac arrest or sudden cardiac death, or autoimmune disorders. Her baseline ECG was normal (Figure 1C), Figure 1. Recurrent fetal demise and fetal arrhythmias. A–C, ECG findings in index case and mother. Leads I, II, and VI are shown for each tracing. A, Index case, 2 weeks of age: 3-lead ECG show- ing prolonged QTc (550 ms) and 2:1 AV block (arrows indicate p waves). B, Infant ECG, showing torsade de pointes arising on a background of ventricular paced rhythm at 150 bpm and preceded by T-wave alternans, a typical feature of LQTS. C, Normal maternal ECG. D, Pedi- gree of proband. Filled symbols indicate intrauterine heart failure; IUFD, intrauter- ine fetal death. 3030 Circulation June 22, 2004 at VA MED CTR BOISE on February 10, 2016http://circ.ahajournals.org/Downloaded from
  3. 3. and her QTc interval did not lengthen abnormally during amiodarone infusion given for treatment of fetal arrhythmias (not shown). Her 1 previous pregnancy had ended in stillbirth at 28 weeks (Figure 1D); a report of the postmortem evaluation of the male fetus described marked cardiomegaly and a supernumerary digit but no other gross abnormalities. Prenatal care was started 10 weeks into her second pregnancy, and all prenatal laboratory studies were normal. The infant’s father was 19 years of age and in excellent health, but he declined to undergo genetic evaluation. The mother’s father and mother were 23 and 18, respectively, at the time of her birth. During these investigations, the mother became pregnant for a third time by a second male partner. Regular prenatal examinations and monthly ultrasound studies revealed no abnormalities until the seventh month. At 28 weeks’ gestation, echocardiography revealed no fetal heartbeat, and a stillborn female infant was delivered by induction. Autopsy of this fetus revealed a severely enlarged but structurally normal heart and nonimmune hydrops fetalis, consistent with low-output cardiac failure; other organ systems were unremark- able. Cord blood was obtained for genetic analysis. All subjects were recruited and evaluated in accordance with regulations set forth by the University of Miami Committee for the Protection of Human Subjects and under a human subjects research protocol approved by the University of Miami Institutional Review Board. Autopsy results on the stillborn fetuses and cord blood from the third fetus were provided with written consent of the mother. DNA Isolation Genomic DNA was obtained from lymphocytes, cultured skin fibroblasts, paraffin-embedded tissue, and buccal swab with a Puregene DNA Isolation Kit (Gentra Systems). DNA yields were determined spectrophotometrically. Single-Stranded Conformational Polymorphism Analysis The first 300 base pairs of exon 28 of SCN5A were amplified with polymerase chain reaction (PCR).3 PCR was also performed on most of the exons from LQT1, LQT2, KCNE1, and KCNE2 with the use of published primers and conditions.3,12 Single-stranded conformational polymorphism (SSCP) analysis was performed on a Bio-Rad Protean II xi Cell electrophoresis apparatus. Gels were made with or without glycerol using MDE Gel Solution (Cambrex BioScience) according to the manufacturer’s recommendations. Gels were developed with a silver stain kit from Bio-Rad according to their instructions. DNA Sequence Analysis PCR products to be sequenced were purified with a QIAquick PCR Purification Kit (Qiagen). Cycle sequencing reactions were per- formed by use of reagents from Applied Biosystems according to their recommendation. The products were resolved on an ABI Prism 3100 Genetic Analyzer and analyzed with ABI Sequence Analysis software. HinfI (10 U/␮L, New England Biolabs) digestions were done for 3 hours at 37°C in 20-␮L reactions with up to 400 ng of PCR product and 10 U of enzyme. Digestion products were analyzed by electrophoresis in 2% agarose gels and staining with ethidium bromide. The intensity of bands was quantified with Sigmascan software (SPSS Science). Results Mutation Analysis To identify the molecular abnormality in the index case, we conducted mutation screening of 5 cardiac ion channel genes, including KCNQ1, HERG, SCN5A, minK, and MiRP-1, cov- ering Ϸ85% of the mutations known to be associated with LQTS.3 Analysis of the proband’s lymphocyte DNA revealed an unusual banding pattern of the first half of exon 28 of SCN5A on SSCP analysis. DNA sequencing of this fragment revealed that the infant was heterozygous for a previously reported mutation at nucleotide 48683,13,14 (Genbank NM_198056), with both a normal guanine residue and a mutant adenine residue (Figure 2A). This introduces a muta- tion in the second position of codon 1623, which changes a positively charged arginine to a neutral glutamine (R1623Q). No mutations were found in any of the other genes investigated. Detection of Maternal Mosaicism Initial SSCP analysis of the mother’s lymphocyte DNA gave an ambiguous result (not shown), but DNA sequencing suggested that she was homozygous for the wild-type allele (Figure 2B and 2E). To resolve this discrepancy, we took advantage of the fact that the R1623Q mutation destroys an Figure 2. Mutation detection in proband, mother, and third fetus. A, B, D, and E, DNA sequence of sense strand of indi- cated source. Arrows show position of mutation at nucleotide 4865. C, PCR products of DNA spanning mutation, after diges- tion with HinfI, amplified from maternal lymphocytes (ML), pro- band lymphocytes (PL), cord blood from third fetus (CB), mater- nal fibroblasts (MF), maternal buccal epithelium (MB), and lymphocytes from 3 unrelated individuals (NC). A, Proband lym- phocyte DNA; B, maternal lymphocyte DNA. D, Maternal DNA resistant to HinfI digestion; E, DNA from unaffected, unrelated individual. Note that intensity of mutant band in stillborn cord blood is intermediate between that of heterozygous brother (PL) and the mosaic mother (ML), possibly reflecting incursion of maternal blood after fetal demise. Miller et al Recurrent Fetal Loss and Maternal LQTS Mosaicism 3031 at VA MED CTR BOISE on February 10, 2016http://circ.ahajournals.org/Downloaded from
  4. 4. HinfI restriction enzyme site (GANTC3AANTC). As ex- pected, the wild-type exon 28 PCR product was completely digested by HinfI, but more than half of the DNA from the index case remained undigested, corresponding to the pres- ence of homoduplex mutant and heteroduplex strands lacking the restriction site (Figure 2C, lanes NC and PL). Analysis of the mother’s lymphocyte DNA also revealed a small amount of material resistant to complete digestion (Figure 2C, lanes ML, MF, and MB). When purified, amplified, and sequenced, this DNA was found to consist almost exclusively of the mutant allele (Figure 2D), consistent with maternal somatic mosaicism for the R1623Q mutation. We next used the HinfI assay to compare levels of mosaicism in several different maternal tissues (Figure 2C). Using a standard curve developed by serial dilution of proband DNA into normal DNA, we determined that the HinfI assay was sufficiently sensitive to permit detection of mutant DNA corresponding to Ϸ4% heterozygous cells. Comparison of DNA from 3 different tissues suggested that mosaicism was greatest in circulating lymphocytes (Ϸ10%), less in buccal epithelium (8%), and least in skin fibroblasts (4%) (Figure 2C). These differences may reflect genuine but small variations in the proportion of mutation-bearing cells or alternatively variability in PCR amplification. A comparably low level of mosaicism in the heart would be consistent with the mother’s lack of symptoms or ECG findings, although this could not be directly confirmed. The mother’s third pregnancy ended in stillbirth at 28 weeks. Analysis of fetal cord blood by HinfI digestion and sequencing revealed the presence of the mutant allele (Figure 2C, lane CB). Discussion In this case report, we describe the late-term loss or near loss of 3 successive pregnancies in a woman with somatic mosaicism for a lethal LQTS mutation. Although material for genetic studies was available only for 2 of the 3 infants, we provide strong evidence for germ-line transfer of the mutation in at least 2 and probably 3 pregnancies. Clinical and postmortem evidence suggests that all 3 pregnancies were complicated by fetal cardiac decompensation, and in the second infant, heart failure was observed to develop in connection with incessant ventricular tachyarrhythmias. Heart failure, bradycardia, cardiac hypertrophy, and tachyarrhythmias are frequent features of the antenatal pre- sentation of LQTS.15 This case provides evidence that late-term fetal loss, like SIDS, can stem from unsuspected LQTS. Fetal demise after the sixth month of gestation is rare, occurring in Ͻ4 per 1000 pregnancies (National Center for Health Statistics, http:// www.cdc.gov/nchs/about/major/fetaldth/abfetal.htm), and known causes (Rh incompatibility, maternal autoimmune disease, thrombophilia, infection with TORCH pathogens, major congenital malformations) were excluded in each of the 3 pregnancies reported here. As with SIDS, fetal LQTS and neonatal LQTS are rarely familial, and death may occur in the absence of a documented ventricular tachyarrhythmia or suggestive family history.16,17 Since Schwartz18 originally proposed that some SIDS cases may reflect congenital LQTS, several lines of supportive evidence have emerged, including an ECG screening study of newborns,19 a case of a near-miss SIDS infant who carried an apparently de novo SCN5A mutation,5 and a case of a SIDS infant in whom postmortem molecular screening identified an apparently de novo muta- tion on KCNQ1.7 More recently, 2 of 93 sequential cases of SIDS were found to have missense mutations in SCN5A.6 The latter study likely underestimates the true contribution of LQTS to unexplained infant death because even in clinically well-defined cases, mutations are not always identified.3,4 Our findings demonstrate that LQTS mutations, particularly those affecting the sodium channel encoded by SCN5A, confer a risk for late-term fetal loss and for infant sudden death. In this case, the mother was found to harbor a small population of cells with the same SCN5A (R1623Q) mutation found in her children, indicating that she is a somatic mosaic for LQTS. Genetic mosaicism results when a mutation occurs in a single cell of the developing embryo and is propagated only to its descendant cells in the adult. Mosaicism can affect either somatic or germ-line lineages or both, depending on the location and embryological stage at which the mutation occurs. It has been estimated that 10% to 20% of ostensibly de novo cases of retinoblastoma,9 hemophilia B,10 Duchenne muscular dystrophy,8 and hemophilia A20 are the result of parental mosaicism for these genetic disorders. Examples of germ-line mosaicism in asymptomatic parents have been documented in several autosomal dominant genetic diseases, including neurofibromatosis,21 osteogenesis imperfecta,22 and hypertrophic cardiomyopathy.23 The R1623Q mutation was first reported in an Asian female infant who, like our proband, presented perinatally with 2:1 AV block, lengthened QT interval, cardiac failure, and life-threatening ventricular arrhythmias. The child is now at least 8 years old and is free of arrhythmias on 600 mg/d mexiletine.24 Our patient is now almost 3 years old and is also free of arrhythmias after receiving an orthotopic heart trans- plant. A third, sporadic instance of this mutation has also been reported in an infant.3,25 The severe phenotype mani- fested by these infants is typical of a subset of LQTS patients who present in fetal and early neonatal life.5,13,16,17,26 A review of 22 case studies from the English language litera- ture16 described a subset of LQTS patients with a severe neonatal-onset phenotype characterized by very long QTc intervals (mean, 665 milliseconds), 2:1 AV block, T-wave alternans, functional bradycardia, and a poor prognosis, with 9 of 22 dead in the first year of life and 50% mortality by 4 years of age. In comparison, a study of LQTS in a large pediatric collaborative study (mean age at diagnosis, 7 years)27 found a mean QTc of 520 milliseconds and a low rate of AV block (4%). A further striking feature of the perinatal LQTS phenotype was the infrequency of a family history of the disease (1 of 22, or 4.5%, compared with 60% in the collaborative study of pediatric LQTS16). The important role of de novo mutations in perinatal LQTS is supported by the fact that disease-causing mutations at the same SCN5A residue (1623) have arisen independently at least 3 times,3,14,25 and a number of other spontaneous mutations in LQTS-associated genes have been linked to lethal arrhyth- 3032 Circulation June 22, 2004 at VA MED CTR BOISE on February 10, 2016http://circ.ahajournals.org/Downloaded from
  5. 5. mias in the fetus and neonate.5,28,29 The poor prognosis of infants with neonatal LQTS undoubtedly also contributes to low heritability. The evidence for SCN5A mutations as a cause of perinatal LQTS seems to conflict with earlier studies showing that patients with LQTS generally have clinical manifestations later in life than patients with mutations at other loci and that SCN5A mutation penetrance is strongly age dependent.30 However, the findings presented here demonstrate that certain SCN5A mutations can have an early, aggressive phenotype characterized by arrhythmias and death before birth or in early infancy. These mutations do not appear to overlap with those previously reported to cause disease later in life.30 Additional work is required to explain the mechanisms underlying these distinct clinical presentations. The mutation detected in our patient lies in the S4 region of SCN5A domain IV, which participates in coupling of channel activation and inactivation.31 DNA sequence alignment of the human isoforms of this sodium channel shows that the 3 brain-type (1A, 2A, and 3A), the skeletal muscle (4A), and the cardiac (5A) isoforms are Ͼ90% identical in this region, suggesting that this part of the protein serves a general and essential function of the channel. The severity of disease associated with mutations in this portion of the channel further underscores its functional importance. In addition to 3 apparently de novo R1623Q mutations being associated with a severe, early-onset phenotype of LQTS (this report and others14,25), the same residue, when mutated to leucine instead of glutamine, also causes the Romano-Ward form of LQTS,3 and another mutation 3 residues away has been associated with the Brugada syndrome.32 Furthermore, mutation of the homologous residue (R1448) in the skeletal SCN4A isoform has been reported to cause paramyotonia congenita,33 a disease characterized by cold-induced myotonia.34 In vitro functional studies on the R1623Q mutant protein have found unique inactivation gating defects that lead to both prolonged spontaneous opening and early reopening, resulting in pro- longed decay of INa.35 These gating defects were sensitive to lidocaine, which restored more normal rapid inactivation kinetics; possibly consistent with this, lidocaine was the only antiarrhythmic agent demonstrating partial clinical benefit in the surviving infant reported here. The role of genetics in fetal demise is likely to be overlooked, particularly when parents have no evidence of disease. Dominant mutations such as those leading to LQTS, hypertrophic cardiomyopathy, and other disorders associated with sudden death are often assumed to have arisen de novo in the affected individual (see elsewhere36 for review). The possibility of genetic mosaicism might not be explored, partly because of technical difficulties in detection and partly because of a low level of suspicion when both parents are clinically normal. Nonetheless, the distinction between de novo mutation and parental germ-line mosaicism is critical because mosaicism confers a considerably higher risk of disease recurrence in subsequent pregnancies. The frequency of de novo or transmitted mosaic LQTS mutations in fetal demise or SIDS remains to be established. Our observation of repeated germ-line transmission of LQTS from a mother with unsuspected mosaicism implies that perinatal cardiac moni- toring, in addition to genetic counseling, should be consid- ered after recurrent late-term fetal loss or infant death. Genetic disorders linked to cardiac sudden death may be an important focus for future genetic epidemiological studies of unexplained third-trimester pregnancy loss. Acknowledgments This work was supported by a collaborative grant from the Miami Heart Research Institute (N.H.B. and R.J.M.), a Grant-in-Aid from the Florida-Puerto Rico Affiliate of the American Heart Association (L.B.), and a generous contribution from the Dr John T. Macdonald Foundation for Medical Genetics. We are grateful to Drs Mark Keating and Igor Splawski for providing control DNA samples, unpublished data, and technical suggestions throughout these exper- iments. We thank Dr Louis Elsas for his review and comments on the manuscript. References 1. 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  7. 7. H. Bishopric Paolo Rusconi, Mary Ellen Ahearn, Lisa Baumbach, Paul Kurlansky, Grace Wolff and Nanette Todd E. Miller, Elicia Estrella, Robert J. Myerburg, Jocelyn Garcia de Viera, Niberto Moreno, Recurrent Third-Trimester Fetal Loss and Maternal Mosaicism for Long-QT Syndrome Print ISSN: 0009-7322. Online ISSN: 1524-4539 Copyright © 2004 American Heart Association, Inc. All rights reserved. is published by the American Heart Association, 7272 Greenville Avenue, Dallas, TX 75231Circulation doi: 10.1161/01.CIR.0000130666.81539.9E 2004;109:3029-3034; originally published online June 7, 2004;Circulation. http://circ.ahajournals.org/content/109/24/3029 World Wide Web at: The online version of this article, along with updated information and services, is located on the http://circ.ahajournals.org//subscriptions/ is online at:CirculationInformation about subscribing toSubscriptions: http://www.lww.com/reprints Information about reprints can be found online at:Reprints: document.Permissions and Rights Question and Answerthis process is available in the click Request Permissions in the middle column of the Web page under Services. Further information about Office. Once the online version of the published article for which permission is being requested is located, can be obtained via RightsLink, a service of the Copyright Clearance Center, not the EditorialCirculationin Requests for permissions to reproduce figures, tables, or portions of articles originally publishedPermissions: at VA MED CTR BOISE on February 10, 2016http://circ.ahajournals.org/Downloaded from

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