EFFECT OF THE DIFFERENT AUXINS
AND CYTOKININS IN CALLUS
INDUCTION, SHOOT,
AND ROOT REGENERATION IN
SUGARCANE
Presented by:...
INTRODUCTION


Modern sugarcane varieties are breeding of
species, involving crosses between the
species Saccharum offici...
SUGARCANE PRODUCER


In 2010, Brazil was the largest producer at 719 million tonnes
(FAO, 2013)
GLOBAL PRODUCTION AND
CONSUMPTION


FAO’s current estimate for world sugar production in 2012/13, at 180
million tonnes, ...
SUGARCANE AREA
HARVESTED
INTRODUCTION
What can we do to improve the quality and
productivity of sugarcane
IMPROVEMENT OF SUGARCANE PRODUCTIVITY AND
QUALITY


Plant improvement
 Conventional

methode

 Plant

biotechnology (ex...
ADVANTAGES OF TISSUE
CULTURE
Plant regeneration through tissue
culture technique would be a better
alternative for improvi...
WHAT IS TISSUE CULTURE ?


Plant tissue culture can be defined as a
procedure used to mantain and grow plant tissue
(call...
PATHWAY OF PLANT TISSUE AND CELL
CULTURE



(Hartmann et.al.,
1990 )
OBJECTIVES OF THE
RESEARCH


To analyze the effect of different concentration
of auxins and cytokinins in Callus Inductio...
MATERIAL


Sugarcane Cultivar CO 671 (3-5 months old)



MS Medium - Murashige and skoog medium



Cytokinin
BAP
 Kn
...
METHODOLOGY
RESULT
CALLUS INDUCTION

Effect of different concentration, auxin and 2,4-D on callus induction
from leaf sheath explants of suga...
CALLUS INDUCTION


The best callus induction suplement




3.0mg/L of 2,4-D = 85% explant produced callus

No callus in...
SHOOT REGENERATION

Effect of the cytokinin (BAP,Kn) and the auxin (IBA,NAA) at different
concentrations and combination i...
SHOOT REGENERATION
cont..

Effect of the cytokinin (BAP,Kn) and the auxin (IBA,NAA) at different
concentrations and combin...
SHOOT REGENERATION


BAP+IBA
 The

best respond 1.0+0.5 mg/L = 95
% produced shoot



BAP+NAA
 The

best respond 1.0+0...
ROOT REGENERATION

Effect of different auxin on formation of root on the invitro grown
micro shoots Cultured
ROOT REGENERATION


The Best Rooting Media




½ strength of MS medium + 3mg/L NAA = 95%
explant produced root

Compari...
CONCENTRATION OF
SUCROSE

Effect of sucrose concentration on rooting of invitro differentiated
shoots of sugarcane
CONCENTRATION OF
SUCROSE


The Best Sucrose Concentration


50 gram/L of sucrose = 96% explant
produced root
pH LEVEL

Effect of different pH level on rooting of in vitro differentiated Shoots of
sugarcane
pH LEVEL


The Best pH Level
 pH



5.7 = 96% explant produced root

The Poorest pH Level
 pH

3 = 0 % explant produce...
CONCLUSION


The best callus induction was observed
 at 3.0 mg/L of 2,4-D



The best shoot regeneration was observed
...
REFERENCE


Gopitha A., Lakshmi B., Senthilmanickam J. 2010. Effect
of the different auxins and cytokinins in callus indu...


Reinert J (1959) The control of morphogenesis
and induction of adventitious embryos in cell
cultures of carrots. Planta...
ACKNOWLEDGMENTS


Kasetsart University Advisor
 Prof.

Sontichai Chanprame, Ph.D
 Prof. Sermsiri Chanprame, Ph.D


Jem...
THANK YOU
FOR
YOUR ATTENTION
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Effect of The Different Auxins and Cytokinins in Callus Induction, Shoot, and Root Regeneration in Sugarcane

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Effect of The Different Auxins and Cytokinins in Callus Induction, Shoot, and Root Regeneration in Sugarcane

  1. 1. EFFECT OF THE DIFFERENT AUXINS AND CYTOKININS IN CALLUS INDUCTION, SHOOT, AND ROOT REGENERATION IN SUGARCANE Presented by: Arghya Narendra Dianastya 5620000087 Departement of Agronomy Kasetsart University, Kamphaeng Saen
  2. 2. INTRODUCTION  Modern sugarcane varieties are breeding of species, involving crosses between the species Saccharum officinarum L. and S. spontaneum L (Heinze,1987).  Sugarcane is a tall growing monocotyledonous crop.  Sugarcane has ability to store high concentrations of sucrose, or sugar, in the internodes of the stem.
  3. 3. SUGARCANE PRODUCER  In 2010, Brazil was the largest producer at 719 million tonnes (FAO, 2013)
  4. 4. GLOBAL PRODUCTION AND CONSUMPTION  FAO’s current estimate for world sugar production in 2012/13, at 180 million tonnes, (new record production)
  5. 5. SUGARCANE AREA HARVESTED
  6. 6. INTRODUCTION What can we do to improve the quality and productivity of sugarcane
  7. 7. IMPROVEMENT OF SUGARCANE PRODUCTIVITY AND QUALITY  Plant improvement  Conventional methode  Plant biotechnology (ex: plant tissue culture)  Cultural practice improvement
  8. 8. ADVANTAGES OF TISSUE CULTURE Plant regeneration through tissue culture technique would be a better alternative for improving the quality, production, and improvement of crop through the rapid production of clones (Jabbot & Atkin, 1987)
  9. 9. WHAT IS TISSUE CULTURE ?  Plant tissue culture can be defined as a procedure used to mantain and grow plant tissue (callus, cells, protoplasm) and organ (stem, root, embryos) in aseptic or in vitro culture.  Basic Concept is Totipotency : Totipotency ability in individual plant cells that can be regenerated to a whole plant by modulating culture conditions (Reinert, 1959).
  10. 10. PATHWAY OF PLANT TISSUE AND CELL CULTURE  (Hartmann et.al., 1990 )
  11. 11. OBJECTIVES OF THE RESEARCH  To analyze the effect of different concentration of auxins and cytokinins in Callus Induction, Shoot, and Root Regeneration in Sugarcane.  To analyze the effect of different level of pH and sucrose in root regenenation of sugarcane.
  12. 12. MATERIAL  Sugarcane Cultivar CO 671 (3-5 months old)  MS Medium - Murashige and skoog medium  Cytokinin BAP  Kn   Auxin IBA  IAA  NAA 
  13. 13. METHODOLOGY
  14. 14. RESULT
  15. 15. CALLUS INDUCTION Effect of different concentration, auxin and 2,4-D on callus induction from leaf sheath explants of sugarcane
  16. 16. CALLUS INDUCTION  The best callus induction suplement   3.0mg/L of 2,4-D = 85% explant produced callus No callus induction  IBA
  17. 17. SHOOT REGENERATION Effect of the cytokinin (BAP,Kn) and the auxin (IBA,NAA) at different concentrations and combination is Ms medium on shoot on shoot regeneration from the callus tissue of sugarcane
  18. 18. SHOOT REGENERATION cont.. Effect of the cytokinin (BAP,Kn) and the auxin (IBA,NAA) at different concentrations and combination is Ms medium on shoot on shoot regeneration from the callus tissue of sugarcane
  19. 19. SHOOT REGENERATION  BAP+IBA  The best respond 1.0+0.5 mg/L = 95 % produced shoot  BAP+NAA  The best respond 1.0+0.5 mg/L = 94% produced shoot
  20. 20. ROOT REGENERATION Effect of different auxin on formation of root on the invitro grown micro shoots Cultured
  21. 21. ROOT REGENERATION  The Best Rooting Media   ½ strength of MS medium + 3mg/L NAA = 95% explant produced root Comparison Media Used  NAA and IBA were better response than IAA for producing roots.
  22. 22. CONCENTRATION OF SUCROSE Effect of sucrose concentration on rooting of invitro differentiated shoots of sugarcane
  23. 23. CONCENTRATION OF SUCROSE  The Best Sucrose Concentration  50 gram/L of sucrose = 96% explant produced root
  24. 24. pH LEVEL Effect of different pH level on rooting of in vitro differentiated Shoots of sugarcane
  25. 25. pH LEVEL  The Best pH Level  pH  5.7 = 96% explant produced root The Poorest pH Level  pH 3 = 0 % explant produced root
  26. 26. CONCLUSION  The best callus induction was observed  at 3.0 mg/L of 2,4-D  The best shoot regeneration was observed  at 1.0 mg/L and 0.5 mg/L of (BAP + NAA)  The best root regeneraration was observed  at 3.0 mg/L of NAA  The best sucrose concentration  50 gram/L of Sucrose  The best pH level  pH 5.7
  27. 27. REFERENCE  Gopitha A., Lakshmi B., Senthilmanickam J. 2010. Effect of the different auxins and cytokinins in callus induction, shoot, root regeneration in sugarcane. International Journal of Pharma and Bio Science. Vol.1/Issue-3/JulSep.2010.
  28. 28.  Reinert J (1959) The control of morphogenesis and induction of adventitious embryos in cell cultures of carrots. Planta 53:318–333.  Jabbott A.J and Atkin R.A. 1987 Improving Vegetatively Propagated Crop. Academic Press Limited.  Hartmann H.T., Kester D.E., Davies F.T., and Geneve R.L. 1997. Plant Propagation: Principles and Practices, Sixth Edition. Prentice Hall International, Inc.
  29. 29. ACKNOWLEDGMENTS  Kasetsart University Advisor  Prof. Sontichai Chanprame, Ph.D  Prof. Sermsiri Chanprame, Ph.D  Jember University Advisor  Dr.  Kacung Haryono CAB (Center of Agriculture Biotechnology) And for All that have helped me to study in Kasetsart University
  30. 30. THANK YOU FOR YOUR ATTENTION

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