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Marsilea structure and reproduction

  2. Marsilea • Occurrence Marsilea is an aquatic or semi aquatic plant. It is common in the temperate regions. It grows in fresh water ponds and ditches in Punjab. Marsilea quadrifolia and Muni lea minuta are commonly found in Pakistan.
  3. General structure • The vegetative plant is a sporophyte. It is differentiated into roots, rhizome and leaves. • 1. Rhizome: The stem is in the form of a rhizome. Rhizome has unlimited growth. Therefore, it covers a very large area. The rhizome is dichotomously branched. It has nodes and internodes. A number of adventitious roots arise at each node on the ventral side. But a single leaf arises at each node from the dorsal side. • 2. Leaves: The leaves are compound. Each leaf has a long petiole and four :carats. The kallets are arranged in cross-like manner at the tip of the petiole. Each leaflet is triangular. Veins form reticulate arrangement Stomata arc located on the dorsal side and ventral side of the leaflets.
  4. Internal Structure of the Rhizome Internally the rhizome is composed of epidermis, cortex and central stele. 1. Epidermis: It forms outer covering. 2. Cortex: The cortex is wide. Its peripheral part consists of parenchymatous cells. Ring of a large air chambers are present around this peripheral portion. This portion is called aerenchyma. It stores air. The inner portion of the cortex is composed Sclerenchymatous cells. Endodermis is present inner side of the cortex. 3. The stele in Marsilea is of amphiphloic solenostele. It has pith in the centre. Protoxylem groups are exarch in position.
  5. Internal structure of leaf Both surfaces of the leaf are bound by epidermis. Epidermis is covered by cuticle. It has sunken stomata. Mesophyll cells are present between both epidermises. Mesophyll cells are differentiated into palisade and spongy cells. Single vein passes through each leaf.
  6. Reproduction Sporocarp Marsilea plant is heterosporous. The megaspores and microspores are produced in megasporangia and microsporangia. Both types of sporangia are found within the same sorus. The sari are produced in hard fruit-bodies called sporocarps. The sporocarps are attached to the base of petioles by short stalks (peduncles). Sporocarp is bean shaped. It has hard and stony wall (capsule).
  7. Its wall has single vascular bundle. The sporocarp has two inner chambers. Each chamber has a row of sori. The sori of two rows alternate with each other. The wall of each sorus is formed by its own indusium. Each sorus contains a row of megasporangia and several microsporangia.
  8. A large placenta is produced on the inner side of the wall in the young sporocarp. The placenta of two sides alternate with each other. Megasporangia and microsporangia are produced on the same placenta. Each placenta is covered by its own individual indusium. Megasporangia mature earlier than the microsporangia. Each megasporangium contains a single megaspore on maturity. But each microsporangium contains several (32-64) microspores. All the tissues except indusia gelatinized in mature sporocarp.
  9. Development of the Sporocarp It is believed that the sporocarp is a single folded pinna. This pinna has single vascular bundle. Receptacles or placentas are produced on the ventral side of this pinna. These receptacles bear sporangial initials. Each receptacle with the developing sporangia forms a An outgrowth is produced towards the midrib side of pinna. outgrowth forms a covering over the sorus. This covering is known as the indusium.
  10. 1 he pima becomes folded towards the ventral side due to the growth of the tissue in the mid dorsal region. The two sides of the pinna meet at the margin. It completely encloses the developing son. The hardening of cells in the wall gives rise to the bean shaped sporocarp.
  11. Dehiscence of sporocarp: The mature sporocarps open after two or three years. The stony wall decay and open the sporocarp. The inner gelatinous material absorbs water and swells. It splits the sporocarp into two valves. The gelatinous cord or sporophore absorbs water and swells. The spores remain viable for a very long period.
  12. Development of the Sporangium 1. The sporangial initial cuts off a jacket initial to the outside. It itself becomes the archesporial initial. 2. The jacket initial divides to produce single layered wall of the sporangium. The archesporial initial cuts off two tapetal cells. These tapetal cells divide to produce two layered tapetum. Tapetum is present inner to the wall. 3. The archesporial initial then divides producing 12-16 spore mother cells. Each spore mother cell undergoes meiosis and produces four spores. The development of megasporangia and microsporangia is similar upto the stage. Both sporangia contain 32 or 64 young spores enclosed in a single layered wall.
  13. 4. The tapetal cells provide nourishment to young spore. So they disintegrate during the development of spores. 5. In the megasporangium only one spore develops further. All others disintegiate forming a mucilaginous mass or plasmodium. This mass provides nourishment to the developing megaspore. In the microsporangium all the spores develop into 32-64 microspores.
  14. Development of the Male Gametophyte: 1. The microspore divides in to two cells. The smaller cell becomes prothalial cell. It is reduced male gametophyte. The larger cell divides in two antheridial initials. 2. Each antheridial initial divides to form three jacket cell and single androgonial initial. The androgonial initial of each antheridium divides to produces 16 androcytes (antherozoid mother cells). Each androcyte changes into antherozoid. 3. The antherozoids have many coils and single flagella. The prothalial cell and the jacket cells of both the antheridia disintegrate. Thus the antherozoids become free in the Surrounding water.
  15. Development of the Female Gametophyte 1. Megaspore divides in to two cells. A smaller cell occupies the whole beak. The larger cell does not divide further. 2. This smaller cell functions as an apical cell. The apical cell divides and cut off form single layered vegetative tissue. The apical cell then functions as an archegonial initial. 3. This archegonial initial divides to produce a small primary cover cell at the top and a central cell at the base.
  16. 4. The cover cell divides to form four neck initials. They divide to form a neck. The central cell divides to produce a small upper primary canal cell and a lower larger primary ventral cell. 5. The primary canal cell divides to produce two neck canal cells. The primary ventral cell divides to produce a lower larger oosphere (egg) and an upper smaller ventral canal cell. 6. The ventral canal cell and the neck canal cells of mature archegonium disintegrate. It forms an opening for the entry of antherozoids
  17. Fertilization Each megaspore is enveloped by a layer of mucilage. Several antherozoids enter into this mucilaginous. One of these antherozoids enters the archegonium and fertilizes the egg to produce oosphere. Development of the Sporophyte The oospore divides to produces four cells. Two sister cells develop stem and cotyledons. The other two cells develop into foot and root. The vegetative cells of the gametophyte form a calyptra. It is two to three cells in thickness. This calyptra forms envelop around the developing embryo. The surface cells of the calyptra produce long rhizoids. Cotyledon and the root grow faster than calyptra and conic out of it. The root enters the soil. Cotyledon expands to form the first simple leaf. Primary root is replaced by adventitious roots. 1 he stem grows horizontally on the soil and form the rhizome.
  18. Alternation of Generation The sporophyte and gametophyte generations alternates with each other. Vegetative plant of Marsilea is a diploid sporophyte. It is hetrosporous. It produces mega and microsspores by meiosis. The spore germinates to form haploid gametoplivte. The gametophyte of Marsilea is dioecious. The microspores give rise to the male gametophyte. The megaspore gives rise to the female gametophyte. Both male and female gametophytes complete their development within the spore walls. Both gametophytes produce male and temale gametes. Gametes fuse to form diploid oospcire. The oospore develops into the sporophyte again.