Anaerobic
respiration:
Fermentation
I can live without YOU!
Scientific Concept:
Anaerobic respiration occurs in conditions of limited
oxygen supply or in the absence of oxygen.
Durin...
ENGAGE
What are the important ingredients do you need to make a bun?
What makes the dough expand and becomes bigger?
How t...
EMPOWER
Carbon dioxide
sensor
Glucose and yeast
mixture solution
Water bath
Procedure:
1. Measure 4 grams of yeast by the electronic balance.
2. Prepare 3 different concentration of glucose solution...
6. Mix the glucose solutions with the yeast in the reagent bottle
under the water bath with temperature 30oC.
7. Add a lay...
Results
Graph of Amount of CO2 Produced Versus Time
5%
10%
15%
Time(s) CO2 I/O-1(ppm) CO2 I/O-2(ppm) CO2 I/O-3(ppm)
0 738.462 590.769 758.154
10 718.769 600.615 758.154
20 738.462 580.9...
Time(s) CO2 I/O-1(ppm) CO2 I/O-2(ppm) CO2 I/O-3(ppm)
310 807.385 689.231 1043.692
320 836.923 679.385 1053.538
330 817.231...
Time(s) CO2 I/O-1(ppm) CO2 I/O-2(ppm) CO2 I/O-3(ppm)
610 945.231 876.308 1595.077
620 974.769 866.462 1624.615
630 964.923...
Time(s) CO2 I/O-1(ppm) CO2 I/O-2(ppm) CO2 I/O-3(ppm)
910 1270.154 1083.077 2520.615
920 1260.308 1083.077 2530.462
930 127...
Time(s) CO2 I/O-1(ppm) CO2 I/O-2(ppm) CO2 I/O-3(ppm)
1360 1772.308 1408 4736
1370 1801.846 1388.308 4775.385
1380 1801.846...
Time(s) CO2 I/O-1(ppm) CO2 I/O-2(ppm) CO2 I/O-3(ppm)
1660 2274.462 1821.538 5041.231
1670 2294.154 1841.231 5060.923
1680 ...
Time(s) CO2 I/O-1(ppm) CO2 I/O-2(ppm) CO2 I/O-3(ppm)
1810 2727.385 2550.154 5070.769
1820 2806.154 2589.538 5051.077
1830 ...
Questions
1. Look at your table ,why does the carbon dioxide level
change?
2. Base on the graph, which fermentation of glu...
Answer
1. The change of carbon dioxide level indicated that the yeast fermentation of
glucose have begun. Or anaerobic res...
ENHANCE
In the production of fruit wine, most fruits have
natural sugar in them and without adding extra
sugar, these can ...
Unique Feature of This Activity
1. Amounts of carbon dioxide produced can be detected and
measured accurately. We can meas...
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Data logging

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Data logging

  1. 1. Anaerobic respiration: Fermentation I can live without YOU!
  2. 2. Scientific Concept: Anaerobic respiration occurs in conditions of limited oxygen supply or in the absence of oxygen. During anaerobic respiration, the glucose is not fully oxidised to carbon dioxide and water but partly disintegrates into ethanol and carbon dioxide in plant cells. C6H12O6 --------------> 2C2H5OH + 2CO2 + 2ATP
  3. 3. ENGAGE What are the important ingredients do you need to make a bun? What makes the dough expand and becomes bigger? How to control the bun expand and becomes bigger in the shortest time?
  4. 4. EMPOWER Carbon dioxide sensor Glucose and yeast mixture solution Water bath
  5. 5. Procedure: 1. Measure 4 grams of yeast by the electronic balance. 2. Prepare 3 different concentration of glucose solutions as follows: 5%, 10% and 15% 3. Boil all the glucose solutions under the Bunsen flame to evaporate any dissolve oxygen in the solution. 4. Cool down the glucose solutions by the ice to prevent the heat produced killed the yeast during the fermentation. 5. Connect the carbon dioxide sensor with the Data Logger.
  6. 6. 6. Mix the glucose solutions with the yeast in the reagent bottle under the water bath with temperature 30oC. 7. Add a layer of oil on the top of the solutions to prevent gas trap on the solution. 8. Seal the carbon dioxide sensor to the reagent bottle of the mixed solution. 9. Put the reagent bottle under the water bath for 10 minutes. 10. Record the amount of carbon dioxide at each 10 second interval.
  7. 7. Results Graph of Amount of CO2 Produced Versus Time 5% 10% 15%
  8. 8. Time(s) CO2 I/O-1(ppm) CO2 I/O-2(ppm) CO2 I/O-3(ppm) 0 738.462 590.769 758.154 10 718.769 600.615 758.154 20 738.462 580.923 748.308 30 738.462 600.615 768 40 708.923 590.769 748.308 50 728.615 610.462 768 60 708.923 590.769 768 70 738.462 610.462 768 80 718.769 600.615 777.846 90 748.308 600.615 777.846 100 738.462 620.308 797.538 110 738.462 610.462 768 120 758.154 620.308 807.385 130 748.308 610.462 797.538 140 768 640 777.846 150 738.462 630.154 807.385 Time(s) CO2 I/O-1(ppm) CO2 I/O-2(ppm) CO2 I/O-3(ppm) 160 777.846 630.154 787.692 170 758.154 649.846 807.385 180 777.846 630.154 787.692 190 777.846 649.846 807.385 200 768 640 807.385 210 787.692 649.846 807.385 220 768 649.846 836.923 230 797.538 640 836.923 240 777.846 669.538 896 250 797.538 649.846 905.846 260 777.846 669.538 935.385 270 807.385 640 974.769 280 817.231 679.385 984.615 290 807.385 669.538 1014.154 300 827.077 669.538 1014.154
  9. 9. Time(s) CO2 I/O-1(ppm) CO2 I/O-2(ppm) CO2 I/O-3(ppm) 310 807.385 689.231 1043.692 320 836.923 679.385 1053.538 330 817.231 689.231 1063.385 340 846.769 689.231 1083.077 350 836.923 699.077 1083.077 360 827.077 708.923 1112.615 370 856.615 689.231 1102.769 380 836.923 728.615 1132.308 390 856.615 708.923 1161.846 400 846.769 748.308 1152 410 876.308 728.615 1191.385 420 856.615 748.308 1191.385 430 866.462 768 1220.923 440 886.154 758.154 1220.923 450 866.462 787.692 1250.462 Time(s) CO2 I/O-1(ppm) CO2 I/O-2(ppm) CO2 I/O-3(ppm) 460 896 787.692 1289.846 470 876.308 807.385 1289.846 480 896 807.385 1309.538 490 876.308 827.077 1309.538 500 905.846 836.923 1358.769 510 896 817.231 1368.615 520 896 846.769 1358.769 530 925.538 827.077 1408 540 905.846 846.769 1417.846 550 925.538 836.923 1457.231 560 915.692 846.769 1447.385 570 935.385 866.462 1476.923 580 925.538 846.769 1516.308 590 955.077 876.308 1536 600 945.231 856.615 1585.231
  10. 10. Time(s) CO2 I/O-1(ppm) CO2 I/O-2(ppm) CO2 I/O-3(ppm) 610 945.231 876.308 1595.077 620 974.769 866.462 1624.615 630 964.923 876.308 1634.462 640 994.462 886.154 1654.154 650 974.769 876.308 1693.538 660 1024 896 1693.538 670 1004.308 866.462 1742.769 680 1014.154 905.846 1762.462 690 1033.846 896 1801.846 700 1024 905.846 1831.385 710 1053.538 915.692 1841.231 720 1043.692 905.846 1910.154 730 1073.231 935.385 1920 740 1043.692 915.692 1979.077 750 1083.077 945.231 1979.077 Time(s) CO2 I/O-1(ppm) CO2 I/O-2(ppm) CO2 I/O-3(ppm) 760 1073.231 925.538 1998.769 770 1083.077 945.231 2048 780 1112.615 955.077 2048 790 1102.769 945.231 2107.077 800 1142.154 974.769 2126.769 810 1132.308 955.077 2176 820 1161.846 974.769 2205.538 830 1142.154 974.769 2254.769 840 1181.538 994.462 2313.846 850 1191.385 1014.154 2323.692 860 1191.385 1004.308 2382.769 870 1220.923 1024 2392.615 880 1211.077 1024 2432 890 1240.615 1063.385 2471.385 900 1240.615 1053.538 2481.231
  11. 11. Time(s) CO2 I/O-1(ppm) CO2 I/O-2(ppm) CO2 I/O-3(ppm) 910 1270.154 1083.077 2520.615 920 1260.308 1083.077 2530.462 930 1270.154 1083.077 2599.385 940 1309.538 1102.769 2609.231 950 1299.692 1092.923 2668.308 960 1348.923 1112.615 2737.231 970 1329.231 1092.923 2766.769 980 1358.769 1112.615 2825.846 990 1348.923 1132.308 2825.846 1000 1398.154 1112.615 2894.769 1010 1388.308 1122.462 2904.615 1020 1398.154 1112.615 2934.154 1030 1437.538 1142.154 3003.077 1040 1427.692 1132.308 3042.462 1050 1467.077 1142.154 3101.538 Time(s) CO2 I/O-1(ppm) CO2 I/O-2(ppm) CO2 I/O-3(ppm) 1060 1447.385 1161.846 3131.077 1070 1486.769 1142.154 3200 1080 1467.077 1161.846 3249.231 1090 1486.769 1152 3268.923 1100 1516.308 1181.538 3318.154 1110 1506.462 1161.846 3347.692 1120 1536 1171.692 3396.923 1130 1526.154 1191.385 3436.308 1140 1555.692 1181.538 3475.692 1150 1545.846 1191.385 3554.462 1160 1575.385 1181.538 3574.154 1170 1565.538 1211.077 3662.769 1180 1575.385 1191.385 3712 1190 1604.923 1220.923 3780.923 1200 1595.077 1230.769 3830.154
  12. 12. Time(s) CO2 I/O-1(ppm) CO2 I/O-2(ppm) CO2 I/O-3(ppm) 1360 1772.308 1408 4736 1370 1801.846 1388.308 4775.385 1380 1801.846 1417.846 4883.692 1390 1831.385 1398.154 4962.462 1400 1831.385 1398.154 5021.538 1410 1860.923 1417.846 5060.923 1420 1880.615 1408 5041.231 1430 1880.615 1437.538 5060.923 1440 1920 1417.846 5041.231 1450 1900.308 1447.385 5060.923 1460 1939.692 1427.692 5060.923 1470 1939.692 1427.692 5041.231 1480 1969.231 1447.385 5060.923 1490 1959.385 1437.538 5041.231 1500 1988.923 1457.231 5060.923 Time(s) CO2 I/O-1(ppm) CO2 I/O-2(ppm) CO2 I/O-3(ppm) 1210 1634.462 1230.769 3869.538 1220 1614.769 1260.308 3928.615 1230 1634.462 1250.462 3948.308 1240 1624.615 1270.154 4017.231 1250 1654.154 1270.154 4056.615 1260 1673.846 1289.846 4135.385 1270 1654.154 1319.385 4214.154 1280 1693.538 1319.385 4263.385 1290 1683.692 1358.769 4361.846 1300 1713.231 1339.077 4401.231 1310 1703.385 1378.462 4460.308 1320 1732.923 1368.615 4489.846 1330 1732.923 1378.462 4548.923 1340 1752.615 1388.308 4617.846 1350 1782.154 1378.462 4657.231
  13. 13. Time(s) CO2 I/O-1(ppm) CO2 I/O-2(ppm) CO2 I/O-3(ppm) 1660 2274.462 1821.538 5041.231 1670 2294.154 1841.231 5060.923 1680 2304 1880.615 5041.231 1690 2343.385 1959.385 5060.923 1700 2343.385 2028.308 5051.077 1710 2382.769 2087.385 5041.231 1720 2392.615 2097.231 5060.923 1730 2441.846 2156.308 5041.231 1740 2441.846 2166.154 5060.923 1750 2481.231 2205.538 5041.231 1760 2520.615 2284.308 5060.923 1770 2550.154 2333.538 5060.923 1780 2619.077 2422.154 5051.077 1790 2638.769 2481.231 5060.923 1800 2707.692 2530.462 5041.231 Time(s) CO2 I/O-1(ppm) CO2 I/O-2(ppm) CO2 I/O-3(ppm) 1510 2008.615 1457.231 5041.231 1520 2008.615 1486.769 5041.231 1530 2048 1476.923 5060.923 1540 2038.154 1496.615 5041.231 1550 2077.538 1536 5060.923 1560 2067.692 1536 5041.231 1570 2097.231 1595.077 5060.923 1580 2107.077 1624.615 5060.923 1590 2116.923 1683.692 5041.231 1600 2146.462 1703.385 5060.923 1610 2136.615 1713.231 5041.231 1620 2185.846 1742.769 5060.923 1630 2185.846 1752.615 5041.231 1640 2225.231 1782.154 5051.077 1650 2235.077 1792 5060.923
  14. 14. Time(s) CO2 I/O-1(ppm) CO2 I/O-2(ppm) CO2 I/O-3(ppm) 1810 2727.385 2550.154 5070.769 1820 2806.154 2589.538 5051.077 1830 2855.385 2658.462 5051.077 1840 2924.308 2727.385 5060.923 1850 3003.077 2845.538 5041.231 1860 3042.462 2924.308 5060.923
  15. 15. Questions 1. Look at your table ,why does the carbon dioxide level change? 2. Base on the graph, which fermentation of glucose produced higher amount of carbon dioxide gas? Why? 3. Calculate the rates of respiration of each concentration of glucose by using the formula given below. 4. What can you conclude from this experiment? Rate of respiration = Final reading- initial reading Time (min)
  16. 16. Answer 1. The change of carbon dioxide level indicated that the yeast fermentation of glucose have begun. Or anaerobic respiration has initiated by yeast. 2. In the same period of time, fermentation of yeast in glucose 5% have produced higher amount of carbon dioxide. This can be explain as in high concentration of glucose such as 10% and 15%, the increase in glucose concentration led to in the increase in fermentation time. Besides, when the glucose content increased, the glucose uptake rate decreased, this will affect the rate of fermentation. Thus, as longer time is required for fermentation of yeast to occur, it have reduced the amount of CO2 produced for both 10% and 15% of glucose fermentation. 4. Fermentation of different concentration of glucose will have different rate of fermentation and different amount of end product in fixed time.
  17. 17. ENHANCE In the production of fruit wine, most fruits have natural sugar in them and without adding extra sugar, these can be turned into a wine of about 4% to 6% volume alcohol. This is a low alcohol content. How high alcohol content of fruit wine is produced?
  18. 18. Unique Feature of This Activity 1. Amounts of carbon dioxide produced can be detected and measured accurately. We can measure in every 10 seconds and the data can be recorded up to three decimal places. 2. Less work need to be done by the students. For example, students do not need to be with the experiment all the time as the results will be recorded automatically during that period of time. 3. The pattern of amount of CO2 production changes can be observe along with the experiment. 4. The changes of the data in table and graph can be observed immediately, allowing us to halt and repeat the experiment easily.

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