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Strain & Screening in Industrial Microbiology


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Strain & Screening in Industrial Microbiology by Dr. Amit Kumar Dutta, Ph.D

Published in: Science
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Strain & Screening in Industrial Microbiology

  1. 1. Industrial Microbiology: Strain & Screening Dr. Amit Kumar Dutta, Ph.D
  2. 2. Important Factor Production of Strain Highly Desirable to use a Production Strain Possessing Strain......!
  3. 3. It should Be High Yielding Strain Have stable Biochemical Characteristics Not produce undesirable substances Be easily cultivated on a large scale Character of Strain Production
  4. 4. Screening Isolation and Detection of high yielding species from the natural source material containing a Heterogeneous Microbial Population is called Screening.
  5. 5. Screening Techniques Many Screening Techniques Usually Two Types of Screening Programmes Primary Screening Secondary Screening
  6. 6. Primary Screening Consist some Elementary Tests required to detect and to isolate new microbial species exhibiting the desired property.
  7. 7. P/S needed in the case of other useful microbial species(MOs capable of producing Organic Acids, Amines, Vitamins, etc). Primary Screening
  8. 8. Evaluation of the Primary Screening in Industrial Research Programmes may be made by citing some specific examples of Screening Procedures. Primary Screening
  9. 9. Screening Procedures of P/S. Crowded Plate Techniques Auxanography Techniques First Plate(A) Second Plate(B) Enrichment Culture Techniques Indicator Dye
  10. 10. Crowded Plate Technique Simplest Screening Technique employed in Detecting & isolating Antibiotic Producers. Preparing a Series of Dilutions of the soil or other source material for the Antibiotic producing Micro-organisms.
  11. 11. Limited Applications Provides information regarding the inhibitory activity of a colony against the unwanted microbes. Crowded Plate Technique
  12. 12. Auxanography Largely Employed for detecting Micro- organisms. Able to Produce Growth Factors Extra- cellularly (Amino Acids, Vitamins)
  13. 13. Enrichment Culture Technique Designed by Beijerinck, Soil Microbiologist. Isolating the desired micro- organisms from a Heterogenous microbial population present in soil.
  14. 14. Unwanted Micro-organisms are eliminated or Develop poorly since they do not find suitable growth conditions in the newly created Environment. Enrichment Culture Technique
  15. 15. Today this Technique has become a valuable tool in many screening programmes meant for isolating industrially important strains. Enrichment Culture Technique
  16. 16. Indicator Dye pH indicating Dyes may be employed in some screening methods for detecting Micro-organisms capable of producing Organic Acids, Amines. Dyes like Neutral Red, Bromthymol Blue etc are added to the Poorly Buffered NAM.
  17. 17. This Differential Technique may also be employed to finding out whether micro-organisms are capable of certain microbial transformations or not. Indicator Dye
  18. 18. Secondary Screening Strictly essential in any systematic screening programme intended to isolate Industrially useful Micro- organisms. Helps in Detecting really useful Micro- organisms in Fermentation Process.
  19. 19. S/S Gives answers to many questions (Final Sorting of Industrially useful Micro-organisms). Secondary Screening
  20. 20. Alternative Screening & Analytical Techniques could be employed for the isolation of microbial isolates important in the production of other Industrial Chemical Substances.
  21. 21. Thank You