Vibrio Cholerae

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Vibrio Cholerae

  1. 1. Awang Ismawi Bin Awang IsmailWan Muhammad Hakimi Bin Wan Zakaria
  2. 2.  Gram negative bacteria Three members pathogenic to human, other species are pathogenic for animal and insects Lead a commensal existance in numerous hosts or occur as saprophytes, particularly in water
  3. 3.  Found in fresh and brackish (slighly salt) water, shellfish and other seafood Man is the major reservoir for V.cholerae Factor of transmission – inadequate sanitation, lack of person and food hygiene, use a polluted water to prepare food, inadequate cooked shellfish etc
  4. 4.  Curved rods with rounded ends 3 x 0.5µm Gram negative – pink stained Actively motile with single polar flagellum. To- and fro- movement (dark-field micsroscopy) Non-capsulated, Non-sporing Some strain is lateral flagellum
  5. 5.  Produce enterotoxin (exotoxin) that activates enzyme adenylcyclase Causing watery diarrhoea producing ‘rice water’ stool containing vibrios, epithelial cells and mucus Adherence factor – motility and extracellular enzymes such as proteases Acute cholera – rapid loss of fluid and electrolytes in stool, vomit leads to muscular cramps and severe dehydration (fatal), acidosis, blood urea increase, urine increase in albumin
  6. 6.  Aerobic Wide temperature. Optimum 37oC Grow in ordinary media but sensitive to acid pH (alkaline; 8.2) 2-3 mm in size after 18 – 24 hr incubation in optimum temp. Low convex with an entire edge, whitish and translucent Older colonies develop a light ochre tinta tube of peptone water with a flake of mucus from stool and incubating for only 6 – 8 hrs
  7. 7.  Ferment glucose, sucrose, mannitol and maltose without gas production Does not utilise lactose or dulcitol Positive in indole and nitrites test Non-hemolytic in sheep blood agar (Greig test) Can be tested in H2SO4

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