Chapter 3 structure and functions


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Chapter 3 structure and functions

  1. 1. Structure and functions The cytoplasmic membrane, also called a cell membrane or plasma membrane. It lies internal to the cell wall and encloses the cytoplasm of the bacterium A. Structure and Composition Like all biological membranes in nature, the bacterial cytoplasmic membrane is composed phospholipid and protein molecules The phospholipid bilayer is arranged so that the polar ends of the molecules (the phosphate and glycerol portion of the phospholipid that is soluble in water) form the outermost and innermost surface of the membrane while the non-polar ends (the fatty acid portions of the phospholipids that are insoluble in water) form the center of the membrane
  2. 2. B. FunctionsThe cytoplasmic membrane is a selectively permeable membranethat determines what goes in and out of the organism . All othermolecules require carrier molecules to transport them through themembrane.Materials move across the bacterial cytoplasmic membrane by passivediffusion and active transport
  3. 3. The Plasma Membrane Phospholipid bilayer Peripheral proteins Integral proteins Transmembrane Proteins Figure 4.14b
  4. 4. The Plasma Membrane Figure 4.14a
  5. 5. Movement of Substances Across Membranes Passive Transport:1. Simple Diffusion2. Facilitated Diffusion3. Osmosis Active Transport:1. Antiporters2. Symporters3. ATP-binding cassette (ABC) system4. Group translocation
  6. 6. 1. Passive Diffusion Passive diffusion is the net movement of gases or small uncharge polar molecules across a phospholipid bilayer membrane from an area of higher concentration to an area of lower concentration
  7. 7. a) Simple diffusion: Movement of a solute from an area of high concentration to an area of low concentration Figure 4.17a
  8. 8. b. Osmosis the diffusion of water across a membrane from an area of higher water concentration(lower solute concentration) to lower water concentration (higher solute concentration). Osmosis is powered by the potential energy of a concentration gradient and does not require the expenditure (belanjawan) of metabolic energy
  9. 9. The Principle of Osmosis Figure 4.18c–e
  10. 10. c. Facilitated Diffusion transport of substances across a membrane by transport proteins, Facilitated diffusion is powered by the potential energy of a concentration gradient and does not require the expenditure (belanjawan) of metabolic energy.
  11. 11. Facilitated Diffusion: Carrier proteinmolecules aid in the movement ofsubstances through cell membranefrom high to low concentration
  12. 12.  Facilitated diffusion: Solute combines with a transporter protein in the membrane Figure 4.17b-c
  13. 13.  Through lipid layer Aquaporins (water channels) Figure 4.17d
  14. 14. Movement of Materials acrossMembranes Active transport: Requires a transporter protein and ATP Group translocation: Requires a transporter protein and PEP
  15. 15. 2. Active Transport Active transport is a process whereby the cell uses both transport proteins and metabolic energy to transport substances across the membrane against the concentration gradient. In this way, active transport allows cells to accumulate (mengumpulkan) needed substances even when the concentration is lower outside. The energy is provided by proton motive force, the hydrolysis of ATP, or the breakdown of some other high-energy compound such as phosphoenolpyruvate (PEP). Proton motive force is an energy gradient resulting from hydrogen ions (protons) moving across the membrane from greater to lesser hydrogen ion concentration. ATP is the form of energy cells most commonly use to do cellular work. PEP is one of the intermediate high-energy phosphate compounds produced during glycolysis.
  16. 16. Active Transport: Carrier Protein molecules aid inmovement of molecules against a concentration gradient
  17. 17.  For the majority of substances a cell needs for metabolism to cross the cytoplasmic membrane, specific transport proteins (carrier proteins) are required. This is because the concentration of nutrients in most natural environments is typically quite low. Transport proteins allow cells to accumulate nutrients from even a space environment. Transport proteins involved in active transport include antiporters, symporters, the proteins of the ATP- binding cassette (ABC) system, and the proteins involved in group translocation .
  18. 18. Antiporter Antiporters are transport proteins that simultaneously transport two substances across the membrane in opposite directions; one against the concentration gradient and one with the concentration gradient. Metabolic energy is required for this type of transport
  19. 19. Symporter Symporters are transport proteins that simultaneously transport two substances across the membrane in the same direction. Symporters use the potential energy of electrochemical gradients from protons (H+), that is, proton motive force to co-transport ions, glucose, and amino acids against their concentration gradient
  20. 20. ATP-binding cassette (ABC) system An example of an ATP-dependent active transport found in various gram-negative bacteria is the ATP- binding cassette (ABC) system. This involves substrate-specific binding proteins located in the bacterial periplasm, the gel-like substance between the bacterial cell wall and cytoplasmic membrane
  21. 21. Step 1 . The periplasmic-binding protein picks up the substance to be transported and carries it to a membrane- spanning transport protein
  22. 22. Step 2 The molecule to be transported across the membrane enters the transporter protein system and a molecule of ATP enters the ATP binding site of the ATP-hydrolyzing protein.
  23. 23. Step 3 Energy provided by the hydrolysis of ATP into ADP, phosphate, and energy moves the molecule across the membrane
  24. 24. Step 4 The carrier protein releases the molecule being transported and the transporter system is ready to be used again.
  25. 25. Group translocation another form of active transport that can occur in prokaryotes. In this case, a substance is chemically altered during its transport across a membrane so that once inside, the cytoplasmic membrane becomes impermeable to that substance and it remains within the cell.
  26. 26. Step 1 When bacteria use the process of group translocation to transport glucose across their membrane, a high-energy phosphate group from phosphoenolpyruvate (PEP) is transferred to the glucose molecule to form glucose-6- phosphate.
  27. 27. Step 2 A high-energy phosphate group from PEP is transferred to the glucose molecule to form glucose-6-phosphate.
  28. 28. Step 3 The glucose-6-phosphate is transported across the membrane.
  29. 29. Step 4 Once the glucose has been converted to glucose-6- phosphate and transported across the membrane, it can no longer be transported back out.