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Abo salt water strain webinar ucsd

  1. 1. Saltwater Resources and Commercial Algae Production Stephen Mayfield San Diego Center for Algae Biotechnology University of California, San Diego
  2. 2. Cyanobacteria Microalgae Macroalgae Produce biodiesel type lipids and biohydrogen Produce sugars for ethanol or biogas What are the Types of Aquatic Biomass? Produce ethanol Lipids and hydrogen Lareefs.comTopnews.in Algae Any of a group of aquatic eukaryotic organisms that carry on photosynthesis Cyanobacteria Photosynthetic prokaryotes found in many environments, often called blue-green algae Red Algae Green Algae Brown Algae Cyanobacteria
  3. 3. Commercial Production in Algae Today Microalgae Annual Production Water Source Application & Product Price (U.S. $) Spirulina 3000 tonnes dry weight Saltwater Human Nutrition Animal Nutrition Cosmetics Phycobiliproteins $53 kg-1 $16 mg-1 Chlorella 2000 tonnes dry weight Freshwater and Saltwater Strains Human Nutrition Cosmetics Aquaculture $53 kg-1 $74 l-1 Dunaliella salina 1200 tonnes dry weight Saltwater Human Nutrition Cosmetics B-carotene $318-$3,186 kg-1 Aphanizomenon flosaquae 500 tonnes dry weight Freshwater Human Nutrition Haematocococcus pluvialis 300 tonnes dry weight Freshwater Aquaculture Astaxanthin $74 l-1 $10,596 kg-1 Crypthecodinium cohnii 240 tonnes DHA oil Saltwater DHA oil $64 g-1 Schizochytrium 10 tonnes DHA oil Saltwater DHA oil $64 g-1 Adapted from: Brennan, L. and Owende, P. (2010) Renewable and Sustainable Energy Reviews. 14: 557-577.
  4. 4. Oil Content of Some Types of Microalgae Microalgae Oil Content (dry wt%) Biomass Yield (g/L) Growth Rate (g/L/D) Botryococcus braunii 25-75 2 Chlorella sp. 28-32 116 .65 Crypthecodinium cohnii 20 15 Dunaliella primolecta 23 30 .41 Isochrysis sp. 25-33 6 .19 Nannochloris sp. 20-35 35 .41 Nannochloropsis sp. 31-68 55 .44 Neochloris oleoabundans 35-54 3.2 .40 Phaeodactylum tricornutum 20-30 42 .32 Tetraselmis sueica 15-23 20 .24 Chlorella.co.nz Starcentral.mbl.edu Bing.com
  5. 5. Productivity is not related to water type Microalgae Oil Content (dry wt%) Biomass Yield (g/L) Growth Rate (g/L/D) Botryococcus braunii 25-75 2 Chlorella sp. 28-32 116 .65 Crypthecodinium cohnii 20 15 Dunaliella primolecta 23 30 .41 Isochrysis sp. 25-33 6 .19 Nannochloris sp. 20-35 35 .41 Nannochloropsis sp. 31-68 55 .44 Neochloris oleoabundans 35-54 3.2 .40 Phaeodactylum tricornutum 20-30 42 .32 Tetraselmis sueica 15-23 20 .24 Chlorella.co.nz Starcentral.mbl.edu Bing.com
  6. 6. Transformations technologies for algae species Species/Variety Class Method Water Type Chlamydomonas reinhardtii Chlorophyceae Bombardment, Electroporation, Freshwater Dunaliella salina Chlorophyceae Electroporation, Bombardment, Glass Beads Saltwater Haematococcus pluvialis Chlorophyceae Bombardment Freshwater Volvox carteri Chlorophyceae Bombardment Freshwater Cyclotella cryptica Diatom Bombardment Saltwater Cylindrotheca fusiformis Diatom Bombardment Saltwater Navicula saprophila Diatom Bombardment Saltwater Phaeodactylum tricornutum Diatom Bombardment Saltwater Thalassiosira pseudonana Diatom Bombardment Saltwater Amphidinium spp. Dinoflagellate Si Carbide Whisker Saltwater Symbiodinium microadriaticum Dinoflagellate Si Carbide Whisker Saltwater Chlorella ellipsoidea Trebouxiophyceae Protoplast Freshwater Chlorella saccharophila Trebouxiophyceae Electroporation Freshwater Chlorella vulgaris Trebouxiophyceae Protoplast Freshwater and Saltwater Chlorella sorokiniana Trebouxiophyceae Bombardment Freshwater Porphyra yezoensis Rhodophyta Bombardment Saltwater Euglena gracilis Euglenaceae Bombardment Freshwater and Saltwater Porphyridium spp. Porphyridiophyceae Bombardment Saltwater
  7. 7. Robust molecular technologies are available for a limited set of algae ER-mCherry mCerulean-NLS 2 color FACS ER-mCherry, mCerulean-NLS 3 color FACS ER-TS-mCherry, mCerulean-NLS, Mito-Venus X Mito-Venus X A B C D E
  8. 8. Engineering of the salt water strain D. tertiolecta for recombinant protein production in chloroplasts
  9. 9. Xylanase Trichoderma reesei Mannases Aspergillus sulphureus Phytase Shigella spp or E. coli Caulobacter crescentus Alpha-galactosidase Lactobacillus acidophilus Trichoderma reesei Analysis of recombinant industrial enzyme production in D. tertiolecta
  10. 10. 1 - Wild type 5 - Galactosidase 2 - Xylanase 6 - Phytase 3 - Manannase 7 - Phytase 4 - Galactosidase Comparison of protein accumulation between C. reinhardtii and D. tertiolecta 1 2 3 4 5 6 7 D. tertiolecta 1 2 3 4 5 6 7 C. reinhardtii
  11. 11. Where do we go from here? Where do we go from here?

Editor's Notes

  • Emphasize that we will mostly be focusing on microalgae!
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