Service de Cytogénétique & d’Embryologie Hôpital Necker-Enfants Malades M Vekemans The Necker Human Embryo Resource Foetal...
Embryo Foetus New- born “ Pre”- embryo Fertilization D1   D15   WG10   WG40   D8 Birth Inner cell mass <ul><li>Preimplanta...
Mostly RU-486 Occasionally aspiration Embryos < 50 days 5-9 wg C9-C19 Late embryos/early foetuses 8-12 wg C18-23+ whole em...
<ul><ul><li>http://embryology.med.unsw.edu.au/embryo.html </li></ul></ul>2 wg 3-4 wg 9-10.5 wg fetus 5-9 wg
 
Distribution of embryo ages
Information database <ul><li>Anonymous identifier  at our end </li></ul><ul><ul><li>only independent abortion clinic can l...
Gene expression:  in situ  hybridization FGF10 , C19 NANOG , C15
Gene expression:  SAGE and microarrays
Gene expression: (q)RT-PCR
Gene translation: immunohistochemistry Synaptogamin (brown) in intestine Neural cell adhesion molecule (white)  in nerves ...
Near-future research avenues <ul><li>Tissue-specific  ch romatin  i mmuno p recipitation </li></ul><ul><ul><li>ChIP-sequen...
Near-future research avenues <ul><li>Cell line derivation </li></ul><ul><ul><li>Functional studies  in vitro </li></ul></u...
© Nick Kim,  http://nearingzero.net/
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Necker Human Embryo Resource

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Talk describing our resource activity to the scientific advisory board of a similar resource in England that was the subject of a European design study, known as DGEmap.

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Necker Human Embryo Resource

  1. 1. Service de Cytogénétique & d’Embryologie Hôpital Necker-Enfants Malades M Vekemans The Necker Human Embryo Resource Foetal pathology Embryology Molecular Genetics G Goudefroye H Etchevers S Thomas C Babarit T Attié-Bitach F Encha-Razavi M Bonnière C Esculpavit J Martinovic
  2. 2. Embryo Foetus New- born “ Pre”- embryo Fertilization D1 D15 WG10 WG40 D8 Birth Inner cell mass <ul><li>Preimplantation genetic diagnosis </li></ul><ul><li>Pluripotent stem cells </li></ul>Embryonic and foetal cells / tissues <ul><li>Gene/protein expression </li></ul><ul><li>Multipotent (stem) cells </li></ul>IVF Elective abortion Spontaneous abortion Medical terminations
  3. 3. Mostly RU-486 Occasionally aspiration Embryos < 50 days 5-9 wg C9-C19 Late embryos/early foetuses 8-12 wg C18-23+ whole embryonic sac 60% recovery entire / pieces fragments organs, parts never whole brain Techniques for elective abortion Whole embryo with trophoblast Pieces (spinal cord)
  4. 4. <ul><ul><li>http://embryology.med.unsw.edu.au/embryo.html </li></ul></ul>2 wg 3-4 wg 9-10.5 wg fetus 5-9 wg
  5. 6. Distribution of embryo ages
  6. 7. Information database <ul><li>Anonymous identifier at our end </li></ul><ul><ul><li>only independent abortion clinic can link to patient </li></ul></ul><ul><ul><li>has been useful once for HIV testing </li></ul></ul><ul><ul><li>a requirement of bioethics law </li></ul></ul><ul><li>Transport conditions: PBS, RPMI, delays </li></ul><ul><li>State, our staging of embryo </li></ul><ul><li>Karyotype and/or anomalies </li></ul><ul><li>Final use: </li></ul><ul><ul><li>fixation (IHC, cold/hot ISH) </li></ul></ul><ul><ul><li>cell culture to derive migratory neural crest cells </li></ul></ul><ul><ul><ul><li>extraction for RNA </li></ul></ul></ul><ul><ul><ul><li>fixation for IHC </li></ul></ul></ul><ul><ul><li>microdissection </li></ul></ul><ul><ul><ul><li>expression studies </li></ul></ul></ul><ul><ul><ul><li>enzyme complex activities </li></ul></ul></ul><ul><ul><li>storage and use of individual slides and tubes </li></ul></ul>
  7. 8. Gene expression: in situ hybridization FGF10 , C19 NANOG , C15
  8. 9. Gene expression: SAGE and microarrays
  9. 10. Gene expression: (q)RT-PCR
  10. 11. Gene translation: immunohistochemistry Synaptogamin (brown) in intestine Neural cell adhesion molecule (white) in nerves and spinal cord
  11. 12. Near-future research avenues <ul><li>Tissue-specific ch romatin i mmuno p recipitation </li></ul><ul><ul><li>ChIP-sequencing </li></ul></ul><ul><li>Many other possibilities </li></ul><ul><ul><li>miRNAs, splice variants… </li></ul></ul>
  12. 13. Near-future research avenues <ul><li>Cell line derivation </li></ul><ul><ul><li>Functional studies in vitro </li></ul></ul><ul><ul><li>Transplantation and function in vivo </li></ul></ul>N-CAM (green), hNCC Fetal pericytes (green) in NOD-SCID mouse model (Crisan et al. 2008)
  13. 14. © Nick Kim, http://nearingzero.net/

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